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A strain of Bacillus subtilis scuec7 and its application

A technology of Bacillus subtilis and strains, applied in the field of microorganisms, to achieve the effects of high-efficiency cellulose degradation activity, accelerated fermentation, and wide application potential

Active Publication Date: 2022-07-12
JIANGSU SHEN LI ECOLOGICAL AGRI TECHCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used hormonal drugs can produce resistance to inflammation, so it is of great significance to develop new drugs that can relax the airway
At present, there is no relevant research report on the application of Bacillus subtilis in the diastolic tracheal ring.

Method used

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  • A strain of Bacillus subtilis scuec7 and its application
  • A strain of Bacillus subtilis scuec7 and its application
  • A strain of Bacillus subtilis scuec7 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Directional enrichment, isolation and identification of Bacillus subtilis SCUEC7 strains

[0026] 1. Directional enrichment and isolation of SCUEC7 strains

[0027] The gastric juice from the healthy bovine rumen was mixed with crop straw for 48h fermentation, 10g of fermented product was taken, 90mL pH5.0 CMC-Na liquid medium (CNB) was added, and the enrichment culture was carried out at 37°C for 12 hours, with a 10-fold gradient of sterile water. serial dilution (10 -1 ~10 -5 ), take 200 μL of diluent and evenly spread it on a CMC-Na solid medium (CNA) plate, incubate at 37 °C for 24 h, add 0.2% Congo red staining solution for 30 min, and wash it off with distilled water and 1 mol / L NaCl solution in turn. Dye solution, pick colonies with larger degradation circles, and then streak multiple times to isolate and purify, and screen out several microorganisms with larger degradation circles. Among them, the diameter of the transparent degradation circle of th...

Embodiment 2

[0038] Example 2 Growth and endoglucanase activity of Bacillus subtilis SCUEC7 strain

[0039] The SCUEC7 strain seed liquid was inoculated into the CMC-Na liquid medium (CNB) with a volume ratio of 0.5%, and the culture was shaken at 37 ° C, and the absorbance value of the bacterial liquid at 600 nm was sampled and measured as the bacterial growth amount. At the same time, the activity of endoglucanase produced was determined. The definition of endoglucanase activity was defined in international units: the amount of enzyme required to catalyze the hydrolysis of CMC-Na to produce 1 μg of glucose per minute was defined as one unit of enzyme activity. (U), the result is as figure 1 Shown: OD of SCUEC7 strain from 0 to 12 h 600 From 0.02 to 3.69, the enzymatic activity of endoglucanase increased from 2.27U / mL to 239.98U / mL. At 12-24h, the OD of SCUEC7 strain 600 Between 3.51 and 3.69, the enzyme activity was between 239.98 U / mL and 218.10 U / mL. Among them, the OD of the SCUEC...

Embodiment 3

[0040] Example 3 Pathogen inhibitory activity of Bacillus subtilis SCUEC7 strain

[0041] 1. Antibacterial activity of SCUEC7 strain against plant pathogens

[0042] Using Botrytis cinerea, Pythium spp., Watermelon Fusarium wilt, Tomato Early Phytophthora, Rhizoctonia solani and Fusarium graminearum as indicator bacteria, the antibacterial activity of SCUEC7 strain against different plant pathogens was studied.

[0043] Take the Bacillus subtilis SCUEC7 strain, culture it on CNB medium at 37°C for 12 hours to obtain a culture solution, then centrifuge the culture solution at 8000 r / min for 10 minutes, take 5 μL of the supernatant of the culture for antibacterial experiments, and measure the diameter of the antibacterial zone , and with the conventional Bacillus subtilis ATCC6633 strain as a control, the measurement results are shown in Table 2: The diameters of the bacteriostatic zone of the bacteria and Fusarium graminearum were: 27.3, 25.2, 12.3, 17.7, 22.2 and 22.0 mm, res...

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Abstract

The present invention discloses a Bacillus subtilis SCUEC7 strain and an application thereof. The Bacillus subtilis SCUEC7 strain has been deposited in the China Center for Type Culture Collection on April 15, 2021, and the deposit number is: CCTCC NO:M 2021377. The SCUEC7 strain provided by the present invention is isolated from the mixed fermentation of bovine gastric juice and crop straw, and it has inhibitory effect on various plant pathogens such as Botrytis cinerea and Pythium, can be used for the prevention and control of crop diseases, and also has an inhibitory effect on the pathogenic bacteria gold Staphylococcus aureus has an inhibitory effect and can be used in the production of food and feed; at the same time, the SCUEC7 strain can also promote the growth of Candida utilis, and its application in feed production can accelerate feed fermentation and effectively improve fermentation. The content of single-cell protein in the feed; and the present invention also finds for the first time that the Bacillus subtilis SCUEC7 strain has a relaxing effect on the pre-contracted tracheal ring of mice with acetylcholine, so it can be prepared as a tracheal ring relaxation drug for the treatment of diseases such as asthma .

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus subtilis SCUEC7 strain and its application. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a kind of Bacillus genus, widely distributed in soil and decaying organic matter, easy to multiply in subtilis infusion, so named. The bacteria are uniformly colored, have no capsule, have flagella, can move, and are Gram-positive. The colony surface is rough and opaque, dirty white or yellowish, and it is aerobic bacteria. It can use protein, various sugars and starch to decompose tryptophan to form indole. Bacillus subtilis can produce endophytic spores and is resistant to heat and stress. Bacillus subtilis has fast growth rate, simple nutritional requirements, easy survival, colonization and reproduction, no pathogenicity, and can secrete a variety of enzymes and antibiotics. Wide range of applications. For example, Bacillus subt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P1/00C12N9/24C12N1/16A23K10/12A61K35/742A61P11/06C12R1/125C12R1/72
CPCC12N1/20A01N63/22C12N9/2405C12N1/16A61K35/742A61P11/06A23K10/12Y02P60/87
Inventor 王力李晓华张建国许琪寻立之冯喆王圣心赵思卡杨佳琪许倍滔
Owner JIANGSU SHEN LI ECOLOGICAL AGRI TECHCO LTD
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