Preparation method and application of critical time-temperature integrator for monitoring aquatic product cold-chain logistics interruption chain condition
A technology of cold chain logistics and critical time, applied to thermometers, thermometers, instruments, etc. that change physically/chemically, can solve problems such as temperature abuse, and achieve the effects of simple preparation, high cost performance, and good color rendering effect
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Embodiment 1
[0016] The critical time-temperature integrator of the present embodiment is made by following method:
[0017] Prepare a phosphate buffer solution with a pH of 7.0 and a concentration of 0.2 mol / l, and sequentially weigh a certain amount of fructose and peptides and dissolve them in the above phosphate buffer solution to form the first aqueous solution with a concentration of 0.4 mol / l and 2.0 mol / l / l of the second aqueous solution. According to the ratio of 1:1, take 400 microliters of the mixture formed by mixing the above two aqueous solutions and add it into the packaging bag to make a critical time-temperature integrator with a size of 25×20mm.
Embodiment 2
[0019] The critical time-temperature integrator of the present embodiment is made by following method:
[0020] Prepare a phosphate buffer solution with a pH of 6.5 and a concentration of 0.1 mol / l, and sequentially weigh a certain amount of fructose and peptides and dissolve them in the phosphate buffer solution to form the first aqueous solution with a concentration of 0.6 mol / l and the first aqueous solution with a concentration of 1.0 mol / l. l of the second aqueous solution. According to the ratio of 1:1, take 400 microliters of the mixture formed by mixing the above two aqueous solutions and add it into the packaging bag to make a critical time-temperature integrator with a size of 25×20mm.
Embodiment 3
[0022] The critical time-temperature integrator of the present embodiment is made by following method:
[0023] Prepare a phosphate buffer solution with a pH of 7.0 and a concentration of 0.05 mol / l, and sequentially weigh a certain amount of fructose and peptides and dissolve them in the phosphate buffer solution to form the first aqueous solution with a concentration of 0.4 mol / l and the first aqueous solution with a concentration of 1.5 mol / l. l of the second aqueous solution. According to the ratio of 1:1, take 400 microliters of the mixture formed by mixing the above two aqueous solutions into the packaging bag to make a critical time-temperature integrator with a size of 25 × 20mm.
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