Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining triazole fungicide in plant-derived food

A plant-derived, triazole-based technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of long time-consuming measurement methods, poor purification effect, and inability to accurately quantitatively measure triazole-based inoculants at the same time.

Pending Publication Date: 2021-09-03
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for measuring triazole fungicides in plant-derived food, to solve the problem that triazole fungicides in crops cannot be accurately and quantitatively measured at the same time, and the determination method takes a long time, and the purification effect is relatively poor. Problems with too much interference and inaccurate measurement results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining triazole fungicide in plant-derived food
  • Method for determining triazole fungicide in plant-derived food
  • Method for determining triazole fungicide in plant-derived food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] The method for measuring triazole fungicides in plant-derived foods in this embodiment uses dried flue-cured tobacco and tea samples as samples to be tested, and the measurement process is as follows: figure 1 As shown, it specifically includes the following steps:

[0047] (1) Pulverization and filtration of the sample: dry and pulverize the sample, and pass through a 2mm sieve to obtain the sample to be processed.

[0048] (2) Sample extraction: Accurately weigh 1.0g of the sample to be processed (accurate to 0.01g) in the extraction chamber 1 of the extraction tube (such as figure 2 As shown), add 10mL of acetonitrile, ultrasonic extraction for 30min (ultrasonic frequency is 40kHz), to obtain the extract.

[0049] (3) Purification of the sample: plug the liquid intake port 6, open the knob 9 between the liquid storage chamber and the purification chamber, put 1.5mL extraction solution in the purification chamber 7, and put 15mg of titanium carbide nanosheets in the...

experiment example

[0066] 1. Sensitivity

[0067] Add different concentrations of furoconazole, metconazole and clotriconazole pesticide standard working solutions to the blank flue-cured tobacco or tea samples, after extraction and purification, inject SFC-MS / MS to generate a signal-to-noise ratio of 3 times (S / N=3) was used as the method limit of detection (LOD), and the concentration yielding 10 times the signal-to-noise ratio (S / N=3) was used as the method limit of quantification (LOQ). When analyzing tea samples, the detection limit of the assay method of the present invention is between 0.006mg / kg-0.008mg / kg, and the quantification limit is between 0.019mg / kg-0.026mg / kg. When analyzing flue-cured tobacco samples, the detection limit of the determination method of the present invention is between 0.007 mg / kg-0.009 mg / kg, and the quantification limit is between 0.023 mg / kg-0.031 mg / kg.

[0068] 2. Accuracy and repeatability

[0069] Add 10 μg / mL standard solution to the cured blank flue-c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Film diameteraaaaaaaaaa
Thicknessaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for determining triazole fungicide in plant-derived food, which belongs to the technical field of physical and chemical inspection of pesticide residues in food. The method for determining the triazole fungicide in the plant-derived food comprises the following steps of S1, extracting a sample with an organic solvent to obtain an extracting solution. S2, purifying the extracting solution through titanium carbide nanosheets, and acquiring a purified solution, S3, filtering the purified liquid to obtain a liquid to be detected, and S4, carrying out supercritical fluid chromatography-tandem mass spectrum analysis on the liquid to be detected, wherein the chromatographic column of the supercritical fluid chromatography in the supercritical fluid chromatography-tandem mass spectrum is Chiralpak AS-3. The determination method has the advantages of being accurate in operation, high in sensitivity and good in repeatability. Meanwhile, the determination method can also realize separation of stereoisomers of three pesticides, namely bromuconazole, metconazole and benzyl chloride triadimenol, the separation speed is high, and the mobile phase is environment-friendly.

Description

technical field [0001] The invention relates to a method for determining triazole fungicides in plant-derived food, and belongs to the technical field of physical and chemical inspection of pesticide residues in food. Background technique [0002] The problem of pesticide residues is an important part of food safety issues, and the index of pesticide residues in food is an important content of food quality control in various countries. Fuconazole, metconazole and clotriconazole are all triazole fungicides, which can effectively control Ascomycetes, Basidiomycetes and Deuteromycetes pathogens on cereal crops, grapes, fruit trees and vegetables. These three pesticides are new fungicides commonly used in crops. If they are used alone for a long time, they will lead to the generation of drug resistance of pathogenic bacteria, resulting in a decrease in the control effect of the compound, an increase in the amount of use, and a significant shortening of the service life. Therefo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N2030/062G01N2030/146Y02P20/54
Inventor 杨飞唐纲岭王颖纪元刘珊珊邓惠敏范子彦边照阳
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com