A high-sensitivity multi-mycotoxin synchronous rapid detection robot and detection method

Abstract

The invention discloses a high-sensitivity multi-mycotoxin synchronous rapid detection robot and a detection method; it includes a reaction filtering device, a driving device and a sample processing device, wherein a plurality of reaction tanks and filtering units are evenly distributed on the edge of the reaction filtering device to Realize simultaneous detection of multiple mycotoxins; the antibody liquid box of the sample processing device is filled with radioactively labeled antibodies, and the mycotoxins in the reaction tank of the reaction filter device undergo specific immune reactions and sinking reactions. The filter unit filters and separates the precipitate produced by the precipitation reaction, elutes the precipitate through the elution device, and dries the precipitate through the hot air drying device, and then detects the radiation intensity by using the luminescent principle of the radiation-excited fluorescent lens. Quantitative calculation of the content of mycotoxins is carried out through the characteristic that the luminous intensity of the fluorescent lens is proportional to the radioactivity, so as to realize the high-sensitivity and rapid quantitative detection of various mycotoxins.

Description

technical field [0001] The invention relates to the field of mycotoxin detection, in particular to a high-sensitivity multi-mycotoxin synchronous rapid detection robot and a detection method. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by certain fungi during the growth process. There are currently more than 300 known types, of which more than 30 mycotoxins are highly toxic to humans and animals, including aflatoxin, maize red Mycelenone, deoxynivalenol, ochratoxin, and fumonisins, etc.; these toxins can widely contaminate food, crops and their products and cause food poisoning. toxicity. [0003] At present, two methods are mainly used for the detection of mycotoxins: chromatography and ELISA. Chromatography has limitations such as cumbersome sample handling, harsh experimental conditions, various experimental equipment, and low detection efficiency. The application scenario of rapid quantitative detection of multiple mycotoxins; and ...

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Problems solved by technology

[0003] At present, two methods are mainly used for the detection of mycotoxins: chromatography and ELISA. Chromatography has limitations such as cumbersome sample handling, harsh experimental conditions, various experimental equipment, and low detection efficiency. The application scenario of rapid quantitative detection of various mycotoxins; and the test paper ELISA cannot fully bind the target mycotoxins in the sample due to the limited number of antibodies carried by the test paper, resulting in poor detection results and unable to determine the mycotoxin content. Accurate quantitative detection

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