High-sensitivity synchronous quick detection robot for multiple mycotoxins and detection method

Abstract

The invention discloses a high-sensitivity synchronous quick detection robot for multiple mycotoxins and a detection method. The robot comprises a reaction filtering device, a driving device and a sample processing device, and a plurality of reaction tanks and filtering units are uniformly distributed on the edge of the reaction filtering device so as to realize simultaneous detection of various mycotoxins; radioactive labeled antibodies are contained in an antibody liquid box of the sample treatment device, mycotoxin in a reaction tank sample of the reaction filtering device is subjected to a specific immunoreaction and a settling section reaction, precipitates generated by a precipitation reaction are filtered and separated through the filtering unit, and the precipitates are eluted through the elution device. After the precipitate is dried by the hot air drying device, the radioactive ray intensity is detected by utilizing the principle that a fluorescent lens is excited by radioactive rays to emit light, and the content of the fungaltoxin is quantitatively calculated according to the characteristic that the luminous intensity of the fluorescent lens is in direct proportion to the radioactivity. Therefore, high-sensitivity rapid quantitative detection of various mycotoxins is realized.

Description

technical field [0001] The invention relates to the field of mycotoxin detection, in particular to a high-sensitivity multi-mycotoxin synchronous rapid detection robot and a detection method. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by certain fungi during the growth process. There are currently more than 300 known types, of which more than 30 mycotoxins are highly toxic to humans and animals, including aflatoxin, maize red Mycelenone, deoxynivalenol, ochratoxin, and fumonisins, etc.; these toxins can widely contaminate food, crops and their products and cause food poisoning. toxicity. [0003] At present, two methods are mainly used for the detection of mycotoxins: chromatography and ELISA. Chromatography has limitations such as cumbersome sample handling, harsh experimental conditions, various experimental equipment, and low detection efficiency. The application scenario of rapid quantitative detection of multiple mycotoxins; and ...

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Problems solved by technology

[0003] At present, two methods are mainly used for the detection of mycotoxins: chromatography and ELISA. Chromatography has limitations such as cumbersome sample handling, harsh experimental conditions, various experimental equipment, and low detection efficiency. The application scenario of rapid quantitative detection of various mycotoxins; and the test paper ELISA cannot fully bind the target mycotoxins in the sample due to the limited number of antibodies carried by the test paper, resulting in poor detection results and unable to determine the mycotoxin content. Accurate quantitative detection

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