Light interference lipid layer composite material as well as construction method and application thereof

A composite material, lipid layer technology, applied in the field of reflection interference, can solve problems such as difficulty, inability to achieve real-time tracking and monitoring, no optical interference characteristics, etc., to achieve the effect of sensitive response

Pending Publication Date: 2021-09-07
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very difficult to prepare a lipid layer and establish a water-oil interface to monitor lipolysis.
Lipid digestion has primarily been studied through in vitro digestion models simulating gastrointestinal transit, and most in vitro lipolysis models follow emulsion microstructure (e.g., droplet size and droplet composition) associated with free fatty acid release, measured by pH titration, Real-time tracking and monitoring in situ cannot be realized
[0004] In summary, the existing lipids do not have light interference characteristics, so there is still a lack of a detection method that can directly track the lipolysis reaction process in situ in real time based on the water-oil interface

Method used

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  • Light interference lipid layer composite material as well as construction method and application thereof
  • Light interference lipid layer composite material as well as construction method and application thereof
  • Light interference lipid layer composite material as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for monitoring lipolysis reaction in situ in real time, comprising the following steps:

[0049] (1) Soak the glass sheet in piranha solution for 120 minutes, rinse it with water three times, and dry it in a fume hood at 115°C to prepare a hydrophilic glass sheet.

[0050] (2) Disperse 0.9 g of silica sphere solution in 100 mL of absolute ethanol to prepare a silica colloidal microsphere / absolute ethanol solution with a mass volume ratio of 0.9%. Put the hydrophilic glass flakes prepared in step (1) in the colloidal silica microsphere / absolute ethanol solution, adopt the vertical evaporation method, control the humidity to be 7.5%, and control the temperature at 20°C. After a week of growth, the Silica colloidal crystal thin films were prepared on the surface of glass flakes. The silica colloidal crystal film was taken out to dry, soaked in piranha solution for 50 minutes, rinsed with water and absolute ethanol three times in sequence, and dried with nitrogen ...

Embodiment 2

[0065] Test method is the same as embodiment 1, wherein in the step (1), the colloidal silica microsphere / dehydrated alcohol solution concentration is changed into 0.8%, in the step (2), in the vertical evaporation method, the air humidity is controlled to be 10%, and the temperature is controlled to be 18°C, soak the silica colloidal crystal film in piranha solution for 40 minutes; in step (3), the alkylating agent is changed to hexamethylenediaminomethyltrimethoxysilane; in steps (7) and (8), The flow rate of the PBS buffer solution and the lipase solution was 0.06mL / min, and the thickness of the silicon dioxide colloidal crystal film was 3500nm.

Embodiment 3

[0067] Test method is the same as embodiment 1, wherein, in the step (1), the colloidal silica microsphere / dehydrated alcohol solution concentration is changed into 1.0%, in the vertical evaporation method in the step (2), the air humidity is controlled to be 8.0%, and the temperature control is At 22°C, soak the silica colloidal crystal film in piranha solution for 70 minutes; in steps (7) and (8), feed PBS buffer solution and lipase at a flow rate of 0.3mL / min to obtain silica colloidal crystals The thickness of the film was 5500 nm.

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Abstract

The invention discloses a light interference lipid layer composite material as well as a construction method and application thereof, a silicon dioxide colloidal crystal film is prepared by utilizing a silicon dioxide microsphere solution, the silicon dioxide colloidal crystal film is subjected to hydrophilization and alkylation hydrophobic treatment, then lipid is loaded on the silicon dioxide colloidal crystal film, and a light interference lipid layer is prepared; and an optical reaction tank is prepared by using the light interference lipid layer, and a reflection interference spectrum of the light interference lipid layer is measured by combining with a microscope. A lipase solution is introduced into an optical reaction tank, and the change of optical thickness, namely real-time information of lipid digestion, is obtained through fitting calculation of a selected interference spectrum band by adopting an extreme value method. The light interference lipid layer is constructed by the lipid-loaded silicon dioxide colloidal crystal film, and the system constructed based on the light interference lipid layer has optical transduction and substrate amplification functions, can sensitively measure lipolysis information in real time and analyze kinetic parameters in the lipolysis process, and has the advantages of simplicity in operation and high sensitivity.

Description

technical field [0001] The invention relates to a light interference lipid layer composite material and its construction method and application, belonging to the technical field of reflection interference. Background technique [0002] Reflectometric Interference Spectroscopy (RIfS) was first named by Professor Gauglitz of the University of Tübingen in Germany and used for in situ real-time non-label analysis of biomolecular interactions at the interface of thin film interference layers. Silica colloidal crystal (SCC) film, as a three-dimensional ordered porous array of monodisperse silicon nanolayers, displays Fabry-Perot interference fringes in reflectance interference spectroscopy. Reflection interference spectroscopy technology system based on silica thin film interference layer provides more possibilities for real-time dynamic sensitive quantitative analysis of biomolecular interactions. However, as a hydrophilic layer, silicon dioxide film is not easy to achieve stabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/55G01N21/01
CPCG01N21/31G01N21/55G01N21/01G01N2021/0112
Inventor 钱卫平周乐乐
Owner SOUTHEAST UNIV
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