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Colloidal gold test strip capable of detecting combined compound of Tg and anti-Tg antibody

A colloidal gold test paper, colloidal gold technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problem of undetectable serum Tg content, etc.

Pending Publication Date: 2021-09-07
SUZHOU DONGNI BIOTECH CO LTD
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Problems solved by technology

Therefore, when DTC recoverers relapse into DTC, although the secretion of Tg increases, due to the presence of TgAb in the body, Tg is quickly bound by the antibody and forms a complex
In this case, serum Tg levels are undetectable using conventional Tg assay kits

Method used

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  • Colloidal gold test strip capable of detecting combined compound of Tg and anti-Tg antibody
  • Colloidal gold test strip capable of detecting combined compound of Tg and anti-Tg antibody
  • Colloidal gold test strip capable of detecting combined compound of Tg and anti-Tg antibody

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Embodiment Construction

[0022] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0023] 1. Antibody screening and preparation

[0024] In order to study the detection antibody of the Tg-anti-Tg antibody complex, we tried various approaches.

[0025] First, try to use various anti-Tg monoclonal antibodies to conduct affinity experiments with Tg-anti-Tg antibody complexes, the results show that all the commercially available anti-Tg monoclonal antibodies and our own developed anti-Tg monoclonal antibodies have no Demonstrate effective antibody titers.

[0026] We studied the reasons in depth, and the results showed that since the antiserum elicited by Tg itself contains anti-Tg polyclonal antibodies, these antibodies form complexes with Tg, covering most of the epitopes on Tg, resulting in the ba...

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Abstract

The invention relates to a colloidal gold test strip capable of detecting a combined compound of Tg and an anti-Tg antibody. The colloidal gold test strip is provided with a detection line, the colloidal gold combined pad contains colloidal gold particles labeled by a combined antibody; a capture antibody is anchored in the detection line, the binding antibody is an anti-Tg polyclonal antibody, and the capture antibody is a mouse anti-human IgG antibody. According to the invention, Tg is used for immunizing an animal which is far away from human genetic relationship, unique antiserum is obtained, and the antiserum and a mouse anti-human IgG antibody can be combined to prepare a colloidal gold test strip for detecting a Tg-anti-Tg antibody compound. By using a specific antiserum combination, the problem that a test strip prepared from single antiserum is poor in correlation in a quantitative detection process is solved. The colloidal gold test strip also has a function of detecting whole blood, and by using the colloidal gold test strip, the Tg-anti-Tg antibody compound and content in peripheral blood of a subject can be rapidly detected, and a convenient tool is provided for monitoring recurrence of a differentiated thyroid cancer convalescent patient.

Description

technical field [0001] The invention relates to the field of thyroid cancer diagnosis, and more particularly relates to a colloidal gold test strip for detecting Tg-anti-Tg antibody complexes. Background technique [0002] Thyroglobulin (Tg) is a glycoprotein secreted by thyroid follicular epithelial cells, with a molecular weight of about 660kD, and is the main component of thyroid follicular colloid. The Tg content in patients with differentiated thyroid cancer (DTC) is elevated, so Tg is a key serological indicator of DTC, which can assist in the initial diagnosis and diagnosis of DTC. After the recovery of DTC patients, due to the high concentration of Tg in the serum, anti-Tg antibodies (TgAb) were induced under the action of the immune system. Therefore, when DTC recoverers relapse into DTC, although the secretion of Tg increases, due to the presence of TgAb in the body, Tg is quickly bound by the antibody and forms a complex. In this case, serum Tg levels were undet...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/558G01N33/543G01N33/68
CPCG01N33/57488G01N33/57407G01N33/68G01N33/558G01N33/54313
Inventor 程若川王珏
Owner SUZHOU DONGNI BIOTECH CO LTD
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