Colloidal gold test strip capable of detecting combined compound of Tg and anti-Tg antibody
A colloidal gold test paper, colloidal gold technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problem of undetectable serum Tg content, etc.
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[0022] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.
[0023] 1. Antibody screening and preparation
[0024] In order to study the detection antibody of the Tg-anti-Tg antibody complex, we tried various approaches.
[0025] First, try to use various anti-Tg monoclonal antibodies to conduct affinity experiments with Tg-anti-Tg antibody complexes, the results show that all the commercially available anti-Tg monoclonal antibodies and our own developed anti-Tg monoclonal antibodies have no Demonstrate effective antibody titers.
[0026] We studied the reasons in depth, and the results showed that since the antiserum elicited by Tg itself contains anti-Tg polyclonal antibodies, these antibodies form complexes with Tg, covering most of the epitopes on Tg, resulting in the ba...
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