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Immunochromatographic test kit for extracting and measuring sugar chain antigens and capable of controlling analyte development

A technology of immunochromatography and sugar chain antigen, which is applied in the field of immunochromatography kits, can solve the problems of decreased sensitivity, non-expansion of samples, and occurrence of non-specific reactions.

Pending Publication Date: 2021-09-07
DENKA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are problems as follows: (1) The sample does not develop; (2) Although it develops, the sensitivity at 5 minutes after the start of the measurement decreases due to the delay in development; (3) Although it develops, a non-specific reaction occurs, etc.

Method used

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  • Immunochromatographic test kit for extracting and measuring sugar chain antigens and capable of controlling analyte development
  • Immunochromatographic test kit for extracting and measuring sugar chain antigens and capable of controlling analyte development
  • Immunochromatographic test kit for extracting and measuring sugar chain antigens and capable of controlling analyte development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Examples of immunochromatographic test strips for group A hemolytic streptococci

[0105] The following nitrites, solid acidic agents, and neutralizing agents were used as the nitrites, solid acidic agents, and neutralizing agents.

[0106] Nitrite (liquid reagent) 3.0M sodium nitrite

[0107] Solid acidic reagent (impregnated in immunochromatographic test piece) 1.0M citric acid

[0108] Neutralization reagent (impregnated in the immunochromatographic test piece) 3.0M Tribasic (Trizma BASE).

[0109] 1. Immobilization of Anti-Streptococcus pyogenes (Group A β-hemolytic Streptococcus) Antibody on Nitrocellulose Membrane (Support)

[0110] Prepare the liquid in which anti-Streptococcus pyogenes antibody was diluted and anti-rabbit IgG antibody, and apply anti-Streptococcus pyogenes antibody linearly on the sample pad side of the nitrocellulose membrane lined with a PET film, and apply anti-Streptococcus pyogenes antibody linearly on the side of the absorption band, res...

Embodiment 2

[0128] The immunochromatography test piece of Example 1 was placed in a test device case (resin case for storing the immunochromatography test piece). The solution containing only 3.0M Na nitrite solution was used as a control, and Triton X-100, benzalkonium chloride, benzethonium chloride, phosphoric acid, sodium hydroxide, NALC, NaCl, PVA, BSA, and NaBr were prepared according to Table 1. The recorded concentration was added to the solution of each condition of 3M Na nitrite solution. Using a negative human nasal fluid sample known to cause a non-specific reaction, a sample was prepared from a Na nitrite solution to which Na nitrite solution and the above-mentioned substances were added (Solution 4: Sample 1) to prepare a sample. 75 µL of the prepared sample was added dropwise, and the judgment was made 5 minutes later.

[0129] As a result, compared with the control condition without addition, under any conditions, the development start time can be shortened, and band dete...

Embodiment 3

[0133] Put the immunochromatographic test piece of Example 1 into the casing of the test device. (1) 2M Na nitrite solution (PVA-free), (2) 2M Na nitrite + 0.5% PVA were prepared as Na nitrite solution. Specimens were prepared by preparing specimens from respective Na nitrite solutions in which live bacteria of Streptococcus pyogenes were added to human throat swab specimens. 75 µL of the prepared sample was added dropwise, and the judgment was made 5 minutes later.

[0134] As a result, in the presence of PVA, the development start time is shortened, and in the case of a positive sample, positive determination can be made from an earlier time period, and the band coloration at the determination time of 5 minutes also becomes faster. Concentrated results. In addition, in the case of test-negative samples, the frequency of non-specific reactions decreased by about 4% (Table 2).

[0135] [Table 2]

[0136]

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Abstract

The present invention aims to provide: an immunochromatographic test kit for controlling the development of analytes on an immunochromatographic test piece and for appropriately controlling processing using acidic reagents, nitrite, and neutralizing reagents; and an immunochromatography method using said kit.The immunochromatographic test kit includes an immunochromatographic test piece that includes: a sample pad to which is added an analyte that has mixed therein (i) a nitrite solution or an acidic solution and (ii) nitrite or an acidic solution; a marker region that includes a marker antibody marking an antibody for sugar chain antigens; and a detection region in which the antibody for sugar chain antigens is immobilized. The immunochromatographic test piece forms an antibody-sugar chain antigen-marker antibody complex in the detection region and measures the sugar chain antigens. The immunochromatographic test piece is for extracting and measuring sugar chain antigens in the analyte and has: a region impregnated with a neutralizing reagent, upstream from the marker region; and, upstream from the region impregnated with the neutralizing reagent, a region impregnated with a solid acidic reagent, when an analyte mixed with nitrite is used, or a region impregnated with nitrite, when an analyte mixed with acidic solution is used. The nitrite or acidic solution include at least one selected from the group consisting of polyoxyethylene octyl phenyl ether, a quaternary ammonium compound, phosphoric acid, sodium hydroxide, N-acetyl-L-cysteine, NaCl, PVA, BSA, and NaBr.

Description

technical field [0001] The invention relates to an immunochromatographic reagent kit for extracting and measuring sugar chain antigens, which can carry out nitrous acid extraction treatment of sugar chain antigens on immunochromatographic test pieces. Background technique [0002] Many rapid diagnostic reagents based on the principle of immunochromatography are widely used as a means to quickly and easily detect viral or bacterial infections to determine treatment options. [0003] A general rapid diagnostic reagent based on the principle of immunochromatography can be quickly and easily measured by suspending a sample in a sample suspension and supplying the suspension to an immunochromatography test strip. [0004] In order to detect microorganisms belonging to the genus Streptococcus such as group A beta-hemolytic streptococci (group A beta-hemolytic streptococci), oral streptococci, it is necessary to extract sugar chain antigens and measure the sugar chain antigens. ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/543G01N33/569
CPCG01N33/54388Y02A50/30G01N33/569G01N1/4044G01N33/5308
Inventor 加藤大介村松志野
Owner DENKA CO LTD
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