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Gynura divaricata zymolyte, and preparation method and application thereof

A technology of Baizicai and Baizi, which is applied in the field of deep processing of traditional Chinese medicinal materials by biotechnology, can solve the problems that diabetes cannot be used, industrial production is difficult to realize, technical requirements and production costs are high.

Pending Publication Date: 2021-09-10
成都恒福禧生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned patented processing method has a long production cycle and a large consumption of raw materials; technical requirements and production costs are very high, and industrial production is not easy to realize, and the finished product is used as a cosmetic application and cannot be used for auxiliary treatment such as diabetes

Method used

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  • Gynura divaricata zymolyte, and preparation method and application thereof
  • Gynura divaricata zymolyte, and preparation method and application thereof
  • Gynura divaricata zymolyte, and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0033] Embodiment 1 The preparation of the enzymatic hydrolyzate of Chinese cabbage leaves of the present invention

[0034] Grind 100 grams of dried white coriander leaves to 60-80 mesh, add 1000 ml of water, keep the temperature at 30-65°C, add 2 grams of cellulase and 1 gram of pectinase, and react for 3 hours; then adjust the pH at the same temperature value to 8 and maintained, add 0.5 g of alkaline protease to react for 2 hours under constant temperature stirring, then add 0.5 g of neutral protease to react for 2 hours; then raise the temperature to 90°C, react at constant temperature for half an hour to inactivate the enzyme, and filter. The supernatant is concentrated under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65° C., and spray-dried to obtain a dry powder of solid glycopeptide (enzymatic hydrolyzate of Azalea leaf). The yield (the weight of the dry powder of the enzymatic hydrolyzate of the leaves of the Chinese cabbage / the ini...

Embodiment 2

[0037] Embodiment 2 The preparation of the hydrolyzate of Chinese cabbage stalks of the present invention

[0038] Grind 100 grams of dried Baizicai stalks to 60-80 mesh, add 1000 ml of water at a constant temperature of 30-65°C, add 2 grams of cellulase and 1 gram of pectinase, and react for 3 hours; then adjust the pH at the same temperature value to 8 and maintained, add 0.4 g of alkaline protease to react for 2 hours under constant temperature stirring, then add 0.4 g of neutral protease to react for 2 hours; then raise the temperature to 90°C, react at constant temperature for half an hour to inactivate the enzyme, and filter. The supernatant is concentrated under reduced pressure at a vacuum degree of 0.06-0.10 Mpa and a temperature of 40-65° C., and spray-dried to obtain a dry powder of the solid glycopeptide (enzymatic hydrolyzate of Azalea stalk) in solid state. Yield (the dry powder weight of the enzymatic hydrolyzate of the leaves of the Chinese cabbage / the initia...

Embodiment 3

[0041] Embodiment 3 Preparation of the whole herb enzymatic hydrolyzate of Chinese cabbage of the present invention

[0042] Grind 100 grams of dried whole herb of Baizicai to 60-80 mesh, add 1000 ml of water at a constant temperature of 30-65°C, add 2 grams of cellulase and 1 gram of pectinase, and react for 3 hours; after that, at the same temperature, adjust The pH value reaches 8 and is maintained. Add 0.5 g of alkaline protease to react for 2 hours under constant temperature stirring, and then add 0.5 g of neutral protease to react for 2 hours; then raise the temperature to 90°C and react at constant temperature for half an hour to inactivate the enzyme and filter. The supernatant is concentrated under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65°C, and spray-dried to obtain a dry powder of the solid-state glycopeptide (enzymatic hydrolyzate of the whole herb of Azalea). Yield (the dry powder weight of the enzymatic hydrolyzate of the who...

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Abstract

The invention discloses Gynura divaricata zymolyte, and a preparation method and application thereof, and belongs to the field of application of biotechnology in deep processing of traditional Chinese medicinal materials. The Gynura divaricata zymolyte is prepared by the following steps of crushing Gynura divaricata, then adding water, sequentially performing enzymolysis with carbohydrase, alkaline protease and neutral protease at 30 to 65 DEG C, performing enzyme inactivation, filtration and drying to obtain the Gynura divaricata zymolyte. The content of polypeptide in the Gynura divaricata zymolyte is 8 to 26 percent (m / m), the content of polysaccharide in the Gynura divaricata zymolyte is 30 to 60 percent (m / m), and the content of flavone in the Gynura divaricata zymolyte is 1.2 to 6.2 percent (m / m). The Gynura divaricata zymolyte disclosed by the invention has a good auxiliary hypoglycemic effect, is beneficial to blood sugar control of patients with diabetes and insulin resistance, and improves the life quality of the patients.

Description

technical field [0001] The invention belongs to the field of deep processing of traditional Chinese medicinal materials by biological technology, in particular to a production method for the deep processing of Chinese cabbage by enzyme engineering technology. Background technique [0002] Baizicai (Gynura divaricata (L.) DC., synonym Gynura ovalis DC.), also known as Jiangtangcao, Baibei notoginseng, chrysanthemum notoginseng, is a plant of the genus Chrysanthemum notoginseng in the family Asteraceae. Baizicai has been introduced in large quantities in my country in recent years, and was approved as a new resource food in 2010. [0003] Baizicai has significant hypoglycemic effect (Hu Yong, Li Weilin, Lin Houwen, et al., Research on the hypoglycemic effect of the aerial part of Radix Notoginseng, Southwest Forestry University Journal, 2007, 27(1): 55-58; Ma Zhengdong, Chen Lei, Song Hong Tao, et al., Hypoglycemic effect and mechanism of water extract of Panax notoginseng on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P3/10A23L33/105A61K127/00A61K135/00
CPCA61K36/28A61P3/10A23L33/105A61K2236/19A23V2002/00A23V2200/328
Inventor 史宗洁张涛朱筱莉张思良周洪云
Owner 成都恒福禧生物技术有限公司
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