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Polyhalogenated aromatic hydrocarbon degrading bacterium and application thereof

A halogenated aromatic hydrocarbon, degrading bacteria technology, applied in bacteria, sustainable biological treatment, microorganisms, etc., can solve problems such as limited degradation capacity

Active Publication Date: 2021-09-14
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is microbial remediation technology or electrodynamic remediation technology, as a single remediation technology, its sustainable and effective degradation ability is still relatively limited.
Existing research and development of halogenated hydrocarbon-degrading bacteria suitable for electric field conditions is still relatively small, especially for relatively high electric field conditions, suitable for a wide range of pH environments, and electrokinetic enhancement under both aerobic and anaerobic conditions The specific facultative degrading bacteria have not been reported

Method used

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  • Polyhalogenated aromatic hydrocarbon degrading bacterium and application thereof
  • Polyhalogenated aromatic hydrocarbon degrading bacterium and application thereof
  • Polyhalogenated aromatic hydrocarbon degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and Screening of Acinetobacter sp. JWDH2-5

[0036]The bacteria source samples came from the contaminated soil 3m below the original factory site of a chemical fuel company in northern China after relocation. Firstly, the polluted soil samples were acclimatized in a DC weak electric field. In order to obtain functional strains adaptable to a wide range of electric field gradients, the electric field acclimatization conditions were set to an electric field condition of 0.5-5V / cm. The change characteristics of the electric field acclimation conditions are as follows: starting from the initial voltage gradient of 0.5V / cm, the electric stimulation treatment is carried out with a voltage gradient of 0.5V / cm increasing step by step. The treatment period of each level is 1h. When the voltage gradient > 2V After / cm, reverse electrode treatment, the electrode switching period is 10min, and the total treatment period of each voltage gradient is still 1h. When...

Embodiment 2

[0041] Example 2 Application of Acinetobacter sp. JWDH2-5 in Electrokinetic Enhanced Microorganism Remediation of Polyhalogenated Aromatic Hydrocarbons Contaminated Soil

[0042] In order to further verify the characteristics of the degradation activity of Acinetobacter sp. JWDH2-5 on polyhalogenated aromatic hydrocarbons in the soil system under electric field conditions, and to prove its ability in the actual engineering application of contaminated soil treatment, the artificial preparation contains 150ppm chlorine Benzene, 150ppm 1,4-dichlorobenzene, 120ppm 1,2,4-trichlorobenzene, 100ppm hexachlorobenzene and other polyhalogenated aromatic hydrocarbons polluted the soil. After preparation, it was aged in the natural state for 1 month before use. The Acinetobacter sp. JWDH2-5 culture solution obtained by alternating anaerobic and aerobic cultures as described in Example 1 in advance was mixed into the contaminated soil, and the mixing amount was correspondingly mixed into 1 L...

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Abstract

The invention belongs to the technical field of halohydrocarbon contaminated soil remediation, and particularly relates to a polyhalogenated aromatic hydrocarbon degrading bacterium and application thereof in contaminated soil remediation. The invention discloses a polyhalogenated aromatic hydrocarbon degrading bacterium acinetobacter sp. JWDH2-5 which is collected in the China Center for Type Culture Collection on April 9, 2021, and the collection number of the polyhalogenated aromatic hydrocarbon degrading bacterium acinetobacter sp. JWDH2-5 is CCTCC NO: M 2021319. The polyhalogenated aromatic hydrocarbon degrading bacterium acinetobacter sp. JWDH2-5 provided by the invention can be used for carrying out anaerobic and aerobic biological metabolism degradation on the polyhalogenated aromatic hydrocarbon, and has a good application potential by aiming at the soil polluted by the polyhalogenated aromatic hydrocarbon in an electric enhanced microbial remediation system.

Description

technical field [0001] The invention belongs to the technical field of halogenated hydrocarbon-contaminated soil remediation, and in particular relates to a polyhalogenated aromatic hydrocarbon-degrading bacterium and its application in remediating polluted soil. Background technique [0002] Industrial development has brought continuous impetus to social and economic growth, but at the same time, the early relatively extensive industrial model has also brought negative impacts on the ecological environment of the development area. Various power fuels, medical pharmaceuticals, antibiotics production, and resistance During the product preparation process of fuel production, pesticide production and other factories, due to product loading and unloading leakage, pipeline transportation accidents, unreasonable discharge of by-products and illegal storage, etc., the soil in and around the factory area is affected by polyhalogenated organic compounds to varying degrees. Pollution,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCC12N1/20B09C1/10Y02W10/10
Inventor 王卅郭书海吴波程凤莲荆佳维
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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