Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for simultaneously detecting contents of effective components in pulse-activating decoction

A technology of active ingredients and Shengmaiyin, which is applied in the field of simultaneous detection of active ingredient content and active ingredient content in Shengmaiyin, can solve the problems of single detection index of Shengmaiyin and unfavorable quality control of Shengmaiyin

Active Publication Date: 2021-09-14
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to comprehensively control the quality of Shengmai Yin (Dangshen Fang), the technical problem to be solved by the present invention is that the detection index of Shengmai Yin (Dangshen Fang) in the prior art is single, which is not conducive to the quality control of Shengmai Yin (Dangshen Fang) , and then provide a rapid, comprehensive and targeted detection method for Shengmaiyin (Dangshen Fang) preparations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously detecting contents of effective components in pulse-activating decoction
  • Method for simultaneously detecting contents of effective components in pulse-activating decoction
  • Method for simultaneously detecting contents of effective components in pulse-activating decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The method for simultaneously detecting the content of active ingredients in Shengmai Drink (Dangshen Prescription) of the present embodiment is to measure 5-hydroxymethylfurfural, protocatechuic acid, syringin, Contents of geniposide, tangshenoside, schisandrin A, and schisandrin B. Specifically include the following steps:

[0057] Step (1) Preparation of reference substance solution:

[0058] Accurately weigh an appropriate amount of 5-hydroxymethylfurfural, protocatechuic acid, syringin, geniposide, tangshenoside, schisandrin A, and schisandrin B reference substances, and add methanol to make 5-hydroxymethyl A solution of 16.00 μg of furfural, 2.52 μg of protocatechuic acid, 1.68 μg of syringin, 3.44 μg of geniposide, 16.12 μg of tangshenoside, 51.96 μg of schisandrin A, and 5.80 μg of schisandrin B was obtained.

[0059] Step (2) preparation of need testing solution:

[0060] Take Shengmaiyin (Dangshen Fang) test sample, take about 0.2mL of this product, put it ...

Embodiment 2

[0070] The method for simultaneously detecting the content of active ingredients in Shengmai Drink (Dangshen Prescription) of the present embodiment is to measure 5-hydroxymethylfurfural, protocatechuic acid, syringin, Contents of geniposide, tangshenoside, schisandrin A, and schisandrin B. Specifically include the following steps:

[0071] Step (1) Preparation of reference substance solution:

[0072] Accurately weigh an appropriate amount of 5-hydroxymethylfurfural, protocatechuic acid, syringin, geniposide, tangshenoside, schisandrin A, and schisandrin B reference substances, and add methanol to make 5-hydroxymethyl A solution of 64.00 μg of furfural, 10.08 μg of protocatechuic acid, 6.72 μg of syringin, 13.76 μg of geniposide, 64.48 μg of tangshenoside, 207.84 μg of schisandrin A, and 58.00 μg of schisandrin B was obtained.

[0073] Step (2) preparation of need testing solution:

[0074] Take the test sample of Shengmaiyin (Dangshen Fang), take about 2.0mL of this produ...

Embodiment 3

[0085] The method for simultaneously detecting the content of active ingredients in Shengmai Drink (Dangshen Prescription) of the present embodiment is to measure 5-hydroxymethylfurfural, protocatechuic acid, syringin, Contents of geniposide, tangshenoside, schisandrin A, and schisandrin B. Specifically include the following steps:

[0086] Step (1) Preparation of reference substance solution:

[0087] Accurately weigh an appropriate amount of 5-hydroxymethylfurfural, protocatechuic acid, syringin, geniposide, tangshenoside, schisandrin A, and schisandrin B reference substances, and add methanol to make 5-hydroxymethyl A solution of 160.00 μg of furfural, 25.20 μg of protocatechuic acid, 16.80 μg of syringin, 34.40 μg of geniposide, 161.20 μg of tangshenoside, 519.60 μg of schisandrin A, and 58.00 μg of schisandrin B was obtained.

[0088] Step (2) preparation of need testing solution:

[0089] Take the test sample of Shengmaiyin (Dangshen Fang), take about 5.0mL of this pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for simultaneously detecting the content of effective components in a pulse-activating decoction. The method comprises the following steps: preparing a test solution, preparing a reference solution and detecting a sample to obtain the effective components of 5-hydroxymethylfurfural, protocatechuic acid, syringin, geniposide, lobetyolin, schisandrin and schisandrol B in the pulse-activating decoction. According to the method disclosed by the invention, the index components in the pulse-activating decoction are simultaneously detected for the first time, and the content of the 5-hydroxymethylfurfural component is quantitatively analyzed, so that the use safety of medicines is ensured, and the quality of the pulse-activating decoction is more comprehensively evaluated and controlled; the selected detection indexes are all components which are relatively high in content in the pulse-activating decoction and have pharmacological activity; a basis can be provided for the safety, effectiveness and accuracy of clinical application of the pulse-activating decoction; the detection time and cost are saved, and the method is accurate, simple and feasible; and the method has the advantages of good stability, good repeatability, high recovery rate and strong specificity.

Description

technical field [0001] The invention relates to a method for simultaneously detecting the content of active ingredients, in particular to a method for simultaneously detecting the content of active ingredients in Shengmai Decoction, and belongs to the field of quality inspection of traditional Chinese medicine preparations. Background technique [0002] Shengmai Decoction (Dangshen Fang) has the effects of invigorating the spleen and lungs, nourishing blood and promoting fluid production. It can be used clinically for both qi and yin deficiency, palpitation, shortness of breath, and slight spontaneous sweating. Codonopsis pilosula is sweet in taste and flat in nature, and belongs to the spleen and lung meridians. Modern pharmacological studies have found that Codonopsis pilosula can enhance physical fitness, improve human immunity, anti-aging, anti-oxidation, anti-fatigue, anti-inflammatory, anti-tumor, enhance memory and improve cognition. Therefore, it is often used as a s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8679G01N2030/027Y02A50/30
Inventor 李俊黄娜娜
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE