Breast milk lipid substitute and preparation method and application thereof
A substitute and lipid technology, applied in the fields of application, dairy products, milk preparations, etc., can solve problems such as incompatibility and large gap in breast milk lipid composition
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Embodiment 1
[0044] The stearic fatty acid composition and distribution of pangasius oil used in this embodiment are detailed in Table 1, and the fatty acid composition of camphor tree seed kernel oil, linseed oil, algae oil and microbial oil are detailed in Table 2. According to the mass ratio of the medium carbon chain fatty acid to the total fatty acid mass ratio is 10%, the mass ratio of linolenic acid to linoleic acid is 2, the mass ratio of ARA to DHA is 1, and the mass ratio of ARA and DHA to the total fatty acid mass ratio is 0.5 , 0.6, 0.7, 0.8, 0.9, and 1.0 fatty acid design amounts Weighed six groups of pangasius fish oil stearin, camphor tree seed oil, linseed oil, algae oil, and microbial oil, and added them to six esterification In the reactor, lipase Lipozyme RM was added according to 10% (w / w) of the mass of the mixed oil. The reaction temperature was 60°C, and the stirring time was 8h. After the transesterification reaction, the lipase in the reaction solution was separat...
Embodiment 2
[0054] The mass ratio of medium-carbon chain fatty acids to total fatty acids is 10%, the mass ratio of linolenic acid to linoleic acid is 2, the mass ratio of ARA to DHA is 1, and the mass ratio of ARA and DHA to total fatty acids is 1 %, take by weighing 148.775g pangasius oil stearin, 25.25g camphor tree seed kernel oil, 75g linseed oil, 7.25g algae oil and 6.275g microbial oil total three groups, add respectively to three same specification esterification reactions Add lipase Lipozyme RM IM, lipase Lipozyme TL IM and lipase Lipozyme RM respectively according to 10% (w / w) of the mixed oil mass. The transesterification reaction conditions are: magnetic stirring (stirrer bar 30 mm×10 mm, rotation speed 100 rpm), reaction temperature 60° C., and reaction time 8 h.
[0055] After the reaction, the transesterification rate was measured by HPLC-ELSD detection method. To compare and analyze the effect of lipase types on transesterification rate, select lipase types. As can be se...
Embodiment 3
[0059] In this example, according to the mass ratio of medium-carbon chain fatty acids to total fatty acids as 10%, the mass ratio of linolenic acid to linoleic acid is 2, the mass ratio of ARA to DHA is 1, and the mass ratio of ARA and DHA respectively accounts for The total fatty acid mass ratio is 1%. Weigh 148.775g of pangasius oil stearin, 25.25g of camphor seed kernel oil, 75g of linseed oil, 7.25g of algae oil and 6.275g of microbial oil, and add them to six groups respectively. In the esterification reactor of the same specification. The transesterification reaction conditions are: lipase Lipozyme RM is 2%-12% (percentage of mixed oil mass), magnetic stirring (stirrer bar 30mm×10mm, rotation speed 100rpm), reaction temperature is 60°C, and reaction time is 8h.
[0060] After the reaction, the transesterification rate was measured by HPLC-ELSD detection method. Comparative analysis of the effect of the amount of enzyme added on the rate of transesterification, to deter...
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