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Modeling method of animal model for kidney essence deficiency with low reproductive function

A technology for reproductive function and deficiency of kidney essence, which is applied in animal husbandry and other fields, can solve the problems of high cost, limited application and popularization, and long modeling time, so as to shorten the modeling time, facilitate popularization and application, and reduce modeling cost Effect

Pending Publication Date: 2021-09-28
陆华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the modeling time of this method is long, the cost is high, and the application and promotion are limited

Method used

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  • Modeling method of animal model for kidney essence deficiency with low reproductive function
  • Modeling method of animal model for kidney essence deficiency with low reproductive function
  • Modeling method of animal model for kidney essence deficiency with low reproductive function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the preparation of the animal model of the deficiency of kidney essence of the present invention with low reproductive function

[0031] 1. Experimental materials

[0032] 1. Experimental animals

[0033] SPF-grade Kunming mice were provided by Sichuan Dashuo Experimental Animal Company, with animal approval number scxk (Chuan 2020-030). Female mice are 6 weeks old and weigh 25±5g; male mice are 8-10 weeks old. The mice had free access to water and food, 12 hours of light per day, the indoor temperature was maintained at 25±1°C, and the relative humidity was maintained at 60%-80%. All experimental animal operations were carried out in accordance with the regulations on the management of experimental animals and relevant operating specifications.

[0034] 2. Experimental drugs and reagents

[0035] The main experimental drugs and reagents used include: pregnant horse serum gonadotropin (PMSG), chorionic gonadotropin (HCG) for injection, hyaluronidase, Q...

Embodiment 2

[0075] Embodiment 2, use the animal model of deficiency of kidney essence of the present invention with low reproductive function to screen drugs

[0076] (1) According to the preparation method of Example 1, an animal model of insufficiency of kidney essence with low reproductive function was established;

[0077] (2) applying the candidate drug to the animal model of insufficiency of kidney essence with low reproductive function established in step (1);

[0078] (3) To observe the effect of candidate drugs on various indicators of kidney-essence insufficiency and low reproductive function, and to evaluate potential drugs for nourishing kidney, nourishing essence, and assisting pregnancy.

[0079] In summary, the present invention has successfully established an animal model of kidney essence deficiency with low reproductive function by repeated ovulation induction. Drugs for nourishing essence and assisting pregnancy are screened. Simultaneously, in the modeling method of ...

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Abstract

The invention provides a modeling method of an animal model for kidney essence deficiency with low reproductive function, and relates to a modeling method of an animal model. According to the modeling method, modeling is carried out through a method of controlled ovarian hyperstimulation for more than three times; and the interval between every two times of controlled ovarian hyperstimulation is 2 days. The animal model for kidney essence deficiency with low reproductive function is successfully established by adopting a repeated controlled ovarian hyperstimulation method, and the model can be used for researching related diseases of kidney essence deficiency with low reproductive function, and can also be used for screening medicines for tonifying kidney, nourishing essence and assisting pregnancy. Meanwhile, in the modeling method, the interval of each controlled ovarian hyperstimulation is only two days, the modeling time is greatly shortened, the modeling cost is reduced, application and popularization are facilitated, and the method has good application prospects.

Description

technical field [0001] The invention relates to a method for making an animal model, in particular to a method for making a model for an animal model of insufficiency of kidney essence accompanied by low reproductive function. Background technique [0002] The kidney is the foundation of congenital nature, the source of heavenly osmanthus, and the foundation of Chongren. "Suwen·Liujie Zangxiang Theory" said: "The kidney is responsible for stinging, the foundation of sealing and storing, and the place of essence." The hair grows long; on the twenty-seventh day, the heaven is guizhi, the Ren meridian is open, the Taichong meridian is prosperous, and the menstrual events are at the current time, so there is a child." "Introduction to Medicine·Qiu Si" said: "Men's essence and women's blood are both qi, blood, yin and yang. Kidney essence is the root of the body's life activities and the foundation of reproduction. [0003] Insufficiency of kidney essence can also be referred ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 陆华刘奕董文然米玲
Owner 陆华