Induction method of ixora flower anther callus

A technique for callus and flower anther, which is applied in the field of inducing the anther callus of Longchuan flower

Active Publication Date: 2021-10-01
MINNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are related studies on the pharmacological characteristics, physiological research, cultivation and application of plants of the genus Dragon Boat, but there is no report on the induction of callus by Dragon Boat Flower anthers.

Method used

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  • Induction method of ixora flower anther callus
  • Induction method of ixora flower anther callus
  • Induction method of ixora flower anther callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In the medium, the influence of different concentration combinations of plant growth regulators on anther culture, MS medium + sucrose 30gL -1 + Activated carbon 0.5g L -1 +Agar 8g L -1 .

[0023] Table 1 Effects of different plant growth regulator concentration combinations on anther culture

[0024]

[0025]

[0026]

[0027] Note: Different lowercase letters indicate significant differences (p<0.05); different uppercase letters indicate extremely significant differences (p<0.01)

[0028] As can be seen from Table 1, when the KT content is 0 mg L -1 , the callus induction rate of Dragon Boat Flower anthers showed an overall upward trend with the increase of 2,4-D concentration. After adding KT to the medium, the induction rate of Dragon Boat Flower Anther callus had a certain promotion effect, and only in The plant growth regulator concentration is 4mg L -1 2,4-D+0.2mgL -1 71.1% at KT, but the induction rate is less than 4mg L -1 2,4-D and 2 mg L -1 ...

Embodiment 2

[0030] Effects of low temperature pretreatment at 4℃ on callus induction in anther culture.

[0031] Table 2 Effect of 4℃ low temperature pretreatment on callus induction of anther culture

[0032]

[0033]

[0034] It can be seen from Table 2 that when 4°C low temperature pretreatment for 48h and 72h, after 40 days of culture, the callus induction rate of Dragon Boat was 63.47% and 64%, respectively, higher than the induction rate of 4°C low temperature pretreatment 0h (47.80% ), but lower than the induction rate (75.00%) of 24h low temperature pretreatment at 4°C.

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Abstract

The invention discloses an induction method of ixora flower anther callus. The method comprises the following steps: performing low-temperature pretreatment on ixora flower buds in a mononuclear period at 4 DEG C, soaking the ixora flower buds in ethanol, soaking the ixora flower buds in a sodium hypochlorite solution, washing the ixora flower buds with sterile water, peeling off the anther, inoculating the anther into an induction culture medium, sealing with a sealing film, and putting into an incubator at 27 + / -1 DEG C for dark culture; the induction culture medium is composed of a basic culture medium and a plant growth regulator, the basic culture medium is formed by adding cane sugar, activated carbon and agar into an MS culture medium, and plant growth agents are 2, 4-D and KT. According to the method, the ixora flower anther is pretreated at the low temperature of 4 DEG C and then is subjected to induction culture in the MS culture medium + 4 mg L<-1> of 2,4-D + 2 mg L<-1> of KT, the induction rate of the ixora flower anther callus is increased, and reference is provided for ixora flower anther culture and breeding.

Description

technical field [0001] The invention relates to the field of dragon boat flower breeding, in particular to a method for inducing dragon boat flower anther callus. Background technique [0002] Ixora chinensis Lam (Ixora chinensis Lam), also known as Bairihong, elixir flower, and Shandan flower, is a plant of Rubiaceae (Rubiaceae) and the genus Ixora Linn. It has bright colors and short plants, and is widely used. Anther culture plays an important role in plant breeding. To study the culture conditions for the anther-induced callus of Dragon Boat Flower, establish the culture system of Dragon Boat Flower anther-induced callus, in order to obtain the basis for the accumulation of Dragon Boat flower haploid materials and double haploid materials. It is conducive to the cultivation of new varieties of dragon boat flowers and the development of corresponding molecular mechanism research. There are about 400 species of Rubiaceae Dracaena plants in the world, most of which are dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005A01H4/008
Inventor 余惠文陆銮眉左梅柯玲俊王玉玲
Owner MINNAN NORMAL UNIV
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