Nano material and preparation method and application thereof

A nanomaterial, peptide polymer technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of inefficient storage of DNA storage time, inefficient storage time, complex DNA, etc., and achieve good biological phase. Capacitance and stability, high anti-counterfeiting ability, huge amount of information

Active Publication Date: 2021-10-01
中科解码北京生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, such methods are often complex, inefficient or do not store DNA for a long time
[0004] In view of the complexity, inefficiency and short storage time of DNA in the existing technology, it is urgent to find a material with good biocompatibility and stability, which can be easily and efficiently co-assembled with DNA to maintain long-term stability

Method used

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  • Nano material and preparation method and application thereof
  • Nano material and preparation method and application thereof
  • Nano material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A polypeptide polymer nanomaterial, the preparation method comprising the following steps: dissolving 1mmol of chitosan in 2mL of deionized water and heating to 50°C, keeping it under stirring for 50min, then cooling to room temperature, adding 6mmol of triethylamine, and slowly Add 4mmol acryloyl chloride, react for 18 hours, dialyze, freeze-dry to obtain the chitosan of acryloyl; finally the chitosan of 0.1mmol acryloyl and molar ratio are 1:1 R1 and R2 (the amount summation of its substance Satisfy 0.3mmol) through the Michael addition method to prepare multifunctional polypeptide polymer nanomaterials. Wherein, R1 is lysine, and R2 is phenylalanine-glycine.

[0065] Wherein, the chitosan structure of acrylylation is shown in the following formula:

[0066]

[0067] NMR image of propionylated chitosan figure 1 shown in figure 1 Among them, the double bond characteristic peak of the NMR peak at ppm=5.8-6.3.

[0068] The structure of the polypeptide polymer nano...

Embodiment 2

[0077] A polypeptide polymer nanomaterial, the preparation method comprising the following steps: dissolving 1mmol chitosan in 2mL deionized water and heating to 50°C, keeping stirring for 30min, then cooling to room temperature, adding 5mmol triethylamine, and slowly Add 3mmol acryloyl chloride, react for 12 hours, dialyze and lyophilize to obtain acryloyl chitosan; finally the 0.1mmol acryloyl chitosan and mol ratio are 10:1 R1 and R2 (the total amount of its substance Satisfy 0.2mmol) through the Michael addition method to prepare multifunctional polypeptide polymer nanomaterials. Wherein R1 is K12, that is, 12 lysines, and R2 is G10, that is, 10 glycines.

[0078] The above-mentioned polypeptide polymer nanomaterial is applied to the step of obtaining nanoparticles in the storage and recognition of DNA, which is the same as that in Example 1.

[0079] The structure of the resulting polypeptide polymer nanomaterial is shown in the following formula:

[0080]

Embodiment 3

[0082] A polypeptide polymer nanomaterial, the preparation method comprising the following steps: dissolving 1mmol of chitosan in 2mL of deionized water and heating to 50°C, keeping it under stirring for 30min, then cooling to room temperature, adding 10mmol of triethylamine, and slowly Add 5mmol acryloyl chloride, react for 24 hours, dialyze, freeze-dry to obtain the chitosan of acryloyl; Finally, the chitosan of 0.1mmol acryloyl and molar ratio are 1:10 R1 and R2 (the amount summation of its substance Satisfy 0.5mmol) through the Michael addition method to prepare multifunctional polypeptide polymer nanomaterials. Wherein R1 is H12, that is, 12 histidines, and R2 is F2G8, that is, 2 phenylalanines and 8 glycines.

[0083] The above-mentioned polypeptide polymer nanomaterials can be applied to the step of obtaining nanoparticles in the storage and recognition of DNA, which is the same as in Example 1.

[0084] The structure of the resulting polypeptide polymer nanomaterial i...

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Abstract

The invention provides a nano material and a preparation method and application thereof, and relates to the field of new materials. The polypeptide polymer nano material is prepared by reacting acryloylated chitosan with a polypeptide sequence under specific conditions, and the material has good biocompatibility and stability, and the material can be co-assembled with DNA so as to keep stable for a long time, is further applied to product anti-counterfeiting, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of new materials, in particular to a nanometer material and its preparation method and application. Background technique [0002] Traditional anti-counterfeiting mainly uses physical methods such as anti-counterfeiting labels, anti-counterfeiting cards, and anti-counterfeiting packaging, relying on subjective methods such as direct observation, touch, and number query by the authenticator. Play the role of anti-counterfeiting. DNA is a kind of biopolymer material that can carry a large amount of information. DNA sequence information can contain a huge amount of information. A DNA chain of length n can theoretically contain a diversity of sequences that can reach 4 n . Natural DNA determines the diversity of species, while synthetic DNA has unlimited possibilities in terms of species. As a key biomolecule, DNA has been widely used in different fields and plays an extremely important role in modern science and techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C08G81/00C12Q1/6806
CPCC08B37/003C08G81/00C12Q1/6806C12Q2527/125
Inventor 段炼
Owner 中科解码北京生物技术有限公司
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