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Method for breeding litopenaeus vannamei seeds with anti-HLV characteristic

A technology of glass seedlings and white prawns, applied in fish farming, application, climate change adaptation, etc., can solve problems such as few can survive healthily, and water environment pollution is aggravated, so as to ensure stable production and supply, improve success rate, well-designed effects

Inactive Publication Date: 2021-10-08
新荣腾种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase of farming scale and high-density farming, the pollution of the water environment has increased, and the diseases in the culture process of Penaeus vannamei have become more and more diverse.
[0003] From the second half of 2019 to the first half of 2020, the "glass seedling" disease broke out from south to north. A large number of shrimps died acutely within 2-3 days, which had a devastating impact on the aquaculture industry. At present, there is no effective control of "glass seedlings". "The effective measures for epidemic diseases, the most effective method is prevention. In actual production, we found that very few of the Penaeus vannamei seedlings have the ability to resist the "glass vaccine" bacteria (HLV), and few of them can be healthy after being infected with HLV. survive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection and temporary raising of seedlings: select 5 million healthy shrimp fry (P5) with a body length of 0.5 cm that are qualified to reach the SPF state through PCR detection, according to 70,000 tails / m 3 The density is evenly divided into four 30m 2 In the cement pool, raise temporarily for 1-2 days. The water must be filtered by Dow ultrafiltration equipment, the water depth is 0.6m, the water temperature is 27-30°C, the salinity is 17-20‰, the dissolved oxygen is above 6mg / L, the pH value is 8.0-8.5, and the total ammonia nitrogen content in the water is 0-0.6 mg / L, light cycle 12h:12h, light intensity 500-1000Lux, feeding 6-8 times a day.

[0026] (2) Toxic attack: After the P5 shrimp seedlings were stabilized for 1-2 days, they were soaked in the HLV bacterial solution to attack the toxin, and the concentration of the toxin attack was 2×10 2 pcs / ml, the preparation method of HLV bacterial liquid is as follows: Yang Feng, a researcher at the Third Instit...

Embodiment 2

[0033] (1) Selection and temporary raising of seedlings: select 5 million healthy shrimp fry (P5) with a body length of 0.5 cm that are qualified to reach the SPF state through PCR detection, according to 70,000 tails / m 3 The density is evenly divided into four 30m 2 In the cement pool, raise temporarily for 1-2 days. The water must be filtered by Dow ultrafiltration equipment. The water depth is 0.6m, the water temperature is 27-30°C, the total ammonia nitrogen content in the water is 0-0.6mg / L, the photoperiod is 12h:12h, the light intensity is 500-1000Lux, and the daily feeding is 6- 8 times.

[0034] (2) Toxic attack: After the P5 shrimp seedlings were stabilized for 1-2 days, they were soaked in the HLV bacterial solution to attack the toxins, and the concentration of the toxins was 4×102 pieces / ml. The preparation method of HLV bacterial liquid is as follows: Yang Feng, a researcher at the Third Institute of Oceanography of the Ministry of Natural Resources, and his sc...

Embodiment 3

[0041] (1) Selection and temporary raising of seedlings: select 5 million healthy shrimp fry (P5) with a body length of 0.5 cm that are qualified to reach the SPF state through PCR detection, according to 70,000 tails / m 3 The density is evenly divided into four 30m 2 In the concrete pool, raise temporarily 1-2 days. The water must be filtered by Dow ultrafiltration equipment, the water depth is 0.6m, the water temperature is 27-30°C, the salinity is 17-20‰, the dissolved oxygen is above 6mg / L, the pH value is 8.0-8.5, and the total ammonia nitrogen content in the water is 0-0.6mg / L, photoperiod 12h: 12h, light intensity 500-1000Lux, feeding 6-8 times a day.

[0042] (2) Toxic attack: After the P5 shrimp seedlings were stabilized for 1-2 days, they were soaked in the HLV bacterial solution to attack the toxins, and the concentration of the toxins was 8×10 2 pieces / ml. The preparation method of HLV bacterial liquid is as follows: Yang Feng, a researcher at the Third Institut...

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Abstract

The invention discloses a method for breeding litopenaeus vannamei seeds with an anti-HLV characteristic. The method comprises the steps of selection of the seeds, challenge, screening, secondary challenge, cultivation of parent prawns, temporary cultivation of the parent prawns and breeding of first filial generation. According to a modern biological principle and a biotechnology method, the first filial generation of prawn seeds bred by parent prawns which are bred through a challenge test and have the anti-HLV characteristic also have very strong anti-HLV epidemic disease performance, stable production and supply of high-quality seeds are guaranteed, and the method also plays an important role in increasing the success rate of commercial prawn culture. The new method for breeding healthy and high-quality seeds is provided, is reasonable in design, has certain operability and is suitable for large-scale productive breeding of the litopenaeus vannamei seeds with the anti-HLV characteristic.

Description

technical field [0001] The invention relates to a method for breeding Penaeus vannamei seedlings with the characteristic of resisting the pathogenic bacteria of glass seedlings, and belongs to the technical field of breeding of Penaeus vannamei disease-resistant traits. Background technique [0002] Penaeus vannamei is an important euryhaline economic shrimp. Because of its fast growth, high yield per unit area and high economic value, it is favored by aquaculture industry, and the scale of farming has grown rapidly. However, with the increase of breeding scale and the aggravation of water environment pollution caused by high-density farming, the diseases in the culture process of Penaeus vannamei are becoming more and more diverse. [0003] From the second half of 2019 to the first half of 2020, the "glass seedling" disease broke out from south to north. A large number of shrimps died acutely within 2-3 days, which had a devastating impact on the aquaculture industry. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59A01K61/13
CPCA01K61/59A01K61/13Y02A40/81
Inventor 谢阳杨丰陈金坤
Owner 新荣腾种业有限公司