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Detection chip and use method thereof

A detection chip and detection layer technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve problems such as the inability to evaluate multiple samples at the same time, and the inability to evaluate the function of a single islet. Achieve the effect of eliminating liquid flow stagnation, increasing shunt entry, and improving detection efficiency

Pending Publication Date: 2021-10-08
BIOISLAND LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection of the islet function evaluation system disclosed in the above-mentioned patents and documents requires a large number of islet samples, and the result reflects the function of a group of mixed islets. It is impossible to evaluate the function of a single islet, and it is also impossible to evaluate multiple samples simultaneously.

Method used

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  • Detection chip and use method thereof
  • Detection chip and use method thereof
  • Detection chip and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] This embodiment provides a detection chip, and the detection chip is composed of a detection layer and a carrier plate.

[0067] Such as figure 1 As shown, the detection layer includes a sequentially connected sampling section 1, a reaction section 2 and a sampling section 3, wherein:

[0068] The sample injection section includes four sample inlets 16 with a diameter of 1.4mm, which are divided into two groups on average. The four first gradual change channels 11 are connected one by one to each of the four sample inlets 16. The width of the first gradual change channels 11 is determined by 0.68mm is gradually reduced to 0.13mm, and the angle between the two first gradient channels 11 connected to each group of sample inlets 16 is 120°. After the first gradual transition channels 11 converge, it becomes a serpentine channel 12 with a diameter of 0.1mm A gas storage cavity 14 is set at the end of the serpentine channel 12, and a plurality of sub-injection channels 15 w...

Embodiment 2

[0077] Example 2: Experimental comparison of whether the four sample outlets of the chip can output liquid and the difference in the amount of liquid output

[0078] Whether the four sample outlets of the chip provided by Comparative Example 1 and the chip provided by Comparative Example 1 (the pipeline has no gradient) can simultaneously output liquid and the difference in the amount of liquid at low flow rates, when the flow rates of the inlets are respectively 800μL / min, 400μL / min, 200μL / min and 40μL / min, theoretically the corresponding flow rate of a single sample outlet among the four sample outlets is 200μL / min, 100μL / min, 50μL / min and 10μL / min respectively.

[0079] The method is as follows: inject deionized water into the injection port at a flow rate of 800 μL / min, 400 μL / min, 200 μL / min, and 40 μL / min, and the injection volume is 400 μL, and check whether the four sample outlets can simultaneously output samples, Sample flow rate and sample volume, the results are sh...

Embodiment 3

[0092] This embodiment provides a method for detecting the secretory function of a single pancreatic islet by using the detection chip of Embodiment 1.

[0093] (1) Manually select a single islet differentiated from stem cells and put it into the reaction pool of the secretory function detection chip in Example 1, and seal the reaction pool;

[0094] (2) first feed KRB solution containing 2mM glucose and incubate for one hour, then continuously feed KRB solution containing 2mM glucose, KRB solution containing 20mM glucose, KRB solution containing 2mM glucose and KRB solution containing 2mM glucose and 30mM potassium chloride KRB solution was used for 30 minutes each, the flow rate of the injection port was 10 μL / min, and samples were collected every 6 minutes.

[0095] (3) The sample is collected through the sample outlet;

[0096] (4) The insulin concentration in the sample was determined by using the human islet detection kit (Ultrasensitive Insulin ELISA Jumbo, brand: Alpc...

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Abstract

The invention provides a detection chip and a use method thereof. The detection chip comprises a detection layer, the detection layer comprises a sample feed section, a reaction section and a sample discharge section which are connected in sequence, and the sample feed section is provided with a serpentine channel. The detection chip can detect secretion activities of cells. The invention further provides a method for detecting a secretion function of a pancreas islet. The method for detecting the secretion function of the pancreas islet can evaluate the secretion function of the single pancreas islet, can realize parallel detection of a plurality of single pancreas islet samples, and can evaluate the number percentage and individual function difference of the effective functional pancreas islets, so that an important reference effect is provided for pancreas islet differentiation research and clinical pancreas islet transplantation.

Description

technical field [0001] The invention belongs to the field of biological detection. Specifically, the present invention relates to a detection chip, its application in detecting the secretory function of cells and a method for detecting the secretory function of pancreatic islets. Background technique [0002] Islet or islet transplantation to replace β cells is the only long-term treatment option for patients with insulin-dependent diabetes. As the evaluation of therapeutic effect before islet transplantation, functional evaluation of islets differentiated from donor or stem cells is generally performed first. [0003] The islet function detection chip is an experimental device used to evaluate the function of isolated islets. Specifically, the islet function detection chip can be used to study the secretory function of different species of islets or islets differentiated from stem cells under different stimulation conditions (such as Insulin and its precursors, glucagon s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34
CPCC12M21/08C12M23/16C12M29/10C12M41/46
Inventor 刘会生尹佳祥林静芳徐涛孟皓
Owner BIOISLAND LAB
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