Unlock instant, AI-driven research and patent intelligence for your innovation.
Synthesis of Multiple Signal Fluorescent Probes and Simultaneous Discrimination of Cys and So 2 , gsh and hcy applications
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A fluorescent probe and multi-signal technology, applied in the field of analytical chemistry, can solve the problem that thiol fluorescent probes cannot be distinguished and detected by four channels at the same time
Active Publication Date: 2022-08-09
HUNAN NORMAL UNIVERSITY
View PDF5 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
In recent years, multi-signal fluorescent probes have attracted extensive attention because they can simultaneously monitor and visualize two or more analytes in living cells, but the reported thiol fluorescent probes have not been able to realize the simultaneous differential detection of Cys using four channels. , SO 2 , GSH and Hcy, four sulfur-containing compounds, develop while distinguishing Cys, SO 2 , GSH and Hcy multi-signal fluorescent probes have great application value in the fields of analysis and detection, environmental science and biochemistry
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0027] Example 1. Synthesis of 4-hydroxy-7-(pyrrolidin-1-yl)-2H-chromen-2-one
[0028] a. Diphenyl malonate (6.28 g, 24.51 mmol) and 3-(pyrrolidin-1-yl)phenol (2 g, 12.25 mmol) were added to dry toluene (20 mL) and the reaction mixture was heated to 110 ℃ for 8 hours,
[0029] b. After the reaction was completed, filter, wash the filter cake with n-hexane 3 times, and dry in vacuo to obtain a pale yellow solid 4-hydroxy-7-(pyrrolidin-1-yl)-2H-chromen-2-one (2.0 g, 68% yield).
Embodiment 2
[0030] Example 2. Synthesis of 4-chloro-2-oxo-7-(pyrrolidin-1-yl)-2H-methylene-3-carbaldehyde
[0031] . Under nitrogen protection, 1.8 mL of dry re-distilled N,N-dimethylformamide (DMF) was slowly added to an equal volume of phosphorus oxychloride (POCl 3 ), stirring at 20-50 °C for 30 minutes to obtain a red solution,
[0032] . 4-Hydroxy-7-(pyrrolidin-1-yl)-2H-chromen-2-one (1.0 g, 4.32 mmol) was dissolved in 5 mL of N,N-dimethylformamide and added dropwise to step In the mixed solution of , the mixture continued to stir and react at 60 °C for 12 hours under nitrogen protection,
[0033] . After the reaction is complete, the steps The reaction solution in the solution was slowly poured into 500 mL of ice water, and the pH was adjusted to 6 with 20% NaOH solution, resulting in a large amount of precipitation, which was filtered, and the filter cake was washed three times with an appropriate amount of deionized water, and the obtained solid was vacuum-dried to obt...
Embodiment 3
[0035] Example 3. Synthesis of 4-(butylthio)-2-oxo-7-(pyrrolidin-1-yl)-2H-methylene-3-carbaldehyde
[0036] . Add 0.20 g (720.19 mmol) 4-chloro-2-oxo-7-(pyrrolidin-1-yl)-2H-methylene-3-carbaldehyde and n-butanethiol 64.95 mg (720.19 mmol) to 20 mL In anhydrousdichloromethane, 70 μL of triethylamine was added dropwise, and the reaction was stirred at room temperature.
[0037] . After the reaction is complete, step 4-(butylthio)-2-oxo-7-(pyrrolidin-1-yl)-2H-methylene-3-carbaldehyde was obtained by column chromatography on the reaction liquid.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for simultaneously distinguishing and detecting cysteine (Cys) and sulfur dioxide (SO2) through different excitation and fluorescence emission signals. 2 ), glutathione (GSH) and a multi-signal fluorescent probe of homocysteine (Hcy), the chemical structural formula of the multi-signal fluorescent probe is as follows: Needle with Cys, NaHSO 3 , GSH and Hcy undergo different chemical reactions to generate different fluorescent substances, so as to distinguish and detect Cys and SO at the same time. 2 , GSH and Hcy, can be used for simultaneous quantification in in vitro environment, simultaneous fluorescence imaging of Hcy, Cys, SO in cells 2 and GSH.
Description
technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to the synthesis of a multi-signal fluorescent probe, and the simultaneous quantitative detection of Cys and SO by the probe in the environment 2 , GSH and Hcy, simultaneously differentiate and image Cys, SO in cells 2 , GSH and Hcy applications. Background technique [0002] Bioactive molecules such as reactive sulfur, reactive nitrogen, and reactive oxygen species play an extremely important role in maintaining the homeostasis of intracellularredox. Among them, as the most abundant active sulfur component (Reactive Sulfur Species: RSS) in intracellular active molecules, it mainly includes biological thiols (cysteine (Cys), homocysteine (Hcy), glutathione ( GSH)), sulfur dioxide (SO 2 ), hydrogensulfide (H 2 S) and active molecules such as N-acetylcysteine (NAC) ( Anal.Chem. 2018, 90 , 533-555.). In mammals, Hcy can be synthesized from methionine with th...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More