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Biological tissue slice sealing liquid

A biological tissue and slicing technology, which is applied in the preparation of test samples, etc., can solve the problems of harmfulness to human health, lack of microscopic clarity of slice preservation, environmental pollution, etc., and achieve high economic and social benefits, preparation and use Simple method, high-definition effect

Pending Publication Date: 2021-10-26
成都骄桐科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional mounting solution is prepared from raw materials such as neutral gum and xylene, which pollutes the environment and is harmful to human health
In the 1980s, some scholars suggested that rosin could be used to prepare mounting solutions, and in recent years, quick-drying mounting solutions are often used, but these two mounting solutions are lacking in terms of section preservation and microscopic clarity.

Method used

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  • Biological tissue slice sealing liquid
  • Biological tissue slice sealing liquid
  • Biological tissue slice sealing liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare biological tissue section mounting solution as follows:

[0026] The solvent isomerized hexadecane and the solute rosin glyceride were uniformly mixed according to the mass ratio of 15.7:7 to obtain the mounting solution A.

Embodiment 2

[0028] The solvent isomerized hexadecane and the solute rosin glyceride were uniformly mixed according to the mass ratio of 17.1:7 to obtain the mounting solution B.

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Abstract

The invention discloses a biological tissue slice sealing liquid, which comprises a solute and a solvent, and the solute comprises rosin glyceride. The biological tissue slice sealing liquid has the advantages that (1) compared with the existing sealing liquid, the biological tissue slice sealing liquid does not pollute the environment, does not harm the health of operation and users, is simple to prepare and use, and has very high economic and social benefits; and (2) compared with the existing quick-drying mounting medium and the mounting medium liquid prepared from rosin, the biological tissue section sealed by the mounting medium liquid has higher tissue cell morphology definition when being observed under a microscope, and the long-term preservation effect of the section is better. Any sheet sealing liquid adopting rosin glyceride as a solute belongs to infringement.

Description

technical field [0001] The invention relates to a technique for preparing biological tissue slices, in particular to a biological tissue slice mounting solution. Background technique [0002] Mounting fluid is also called mounting agent. As the name suggests, it can seal the stained tissue between the glass slide and the cover glass without direct contact with the air, avoid oxidation and fading, and facilitate the long-term preservation and maintenance of slices. reuse. On the other hand, under the enrichment of the mounting medium, the refractive index of the tissue section can be close to that of the glass slide, so as to obtain a clear microscopic examination effect. [0003] When making slices, the biological tissue is usually sliced ​​after processing, and the biological tissue slice is mounted on a glass slide for staining and other treatments, then dripped with mounting fluid, and then covered with a cover glass, which completes the production of biological tissue s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/36
CPCG01N1/28G01N1/36
Inventor 王文洲
Owner 成都骄桐科技有限公司