An antibacterial material based on nano silver and photodynamic therapy and its preparation method and application

A nano-antibacterial material, antibacterial material technology, applied in the direction of antibacterial drugs, wave energy or particle radiation treatment materials, medical preparations of non-active ingredients, etc., can solve the problem of poor water solubility, insufficient bactericidal effect to remove biofilm, etc. problems, to achieve the effect of superior biofilm removal ability, high efficiency removal, and efficiency improvement

Active Publication Date: 2022-08-05
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photosensitizer Ce6 is currently a commonly used photosensitizer, but its water solubility is not good, and the bactericidal effect of a single photodynamic therapy is not enough to remove biofilm

Method used

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  • An antibacterial material based on nano silver and photodynamic therapy and its preparation method and application
  • An antibacterial material based on nano silver and photodynamic therapy and its preparation method and application
  • An antibacterial material based on nano silver and photodynamic therapy and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A preparation method of a novel nanometer antibacterial material, comprising the following steps:

[0038] 2 mg Ce6 was dissolved in 167 μL of dimethyl sulfoxide, followed by 5 mL of ethanol. To ethanol was added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (10 μM), N -Hydroxysuccinimide (10 μM) and triethylamine (10 μM) were stirred at room temperature. After 2 h of reaction, PEI was added to the Ce6 ethanol solution and stirred overnight. The Ce6 without PEI was removed with a dialysis bag, and stored in a refrigerator at 4 °C in the dark.

[0039]Under nitrogen protection, silver nitrate solution (5 mmol) was added to a 50 mL three-neck flask, 3-mercapto-1-propanesulfonate (0.5940 mg) was dissolved in 2 mL of deionized water, and it was dispersed by ultrasonication for more than 10 s Homogeneous, added to the silver nitrate solution with magnetic stirring for 10 min; sodium borohydride (0.1033 g) was dissolved in 6 mL of deionized water and added dr...

Embodiment 2

[0044] A preparation method of a novel nanometer antibacterial material, comprising the following steps:

[0045] 2 mg Ce6 was dissolved in 167 μL of dimethyl sulfoxide, followed by 5 mL of ethanol. To ethanol was added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (10 μM) and N -Hydroxysuccinimide (10 μM) and N , N - Diisopropylethylamine (10 μM) stirred at room temperature. After 2 h of reaction, PEI was added to the Ce6 ethanol solution and stirred overnight. The Ce6 without PEI was removed with a dialysis bag, and stored in a refrigerator at 4 °C in the dark.

[0046] Under nitrogen protection, silver nitrate solution (5 mmol) was added to a 50 mL three-neck flask, sodium citrate (0.004 g) was dissolved in 2 mL of deionized water, sonicated to make it uniformly dispersed, and added to the silver nitrate solution with magnetic stirring 10 min; sodium borohydride (0.1033 g) was dissolved in 6 mL of deionized water and added dropwise to the silver nitrate s...

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Abstract

The invention discloses an antibacterial material based on nano silver and photodynamic therapy, a preparation method and application thereof, and belongs to the field of biological nanometer antibacterial materials. The antibacterial material includes polyethyleneimine PEI, Ce6 and silver nanoparticle AgNPs; Ce6 and the amino group on the polyethyleneimine PEI molecular chain are connected by covalent bonds, and the silver nanoparticle AgNPs is encapsulated in the polyethyleneimine connected with Ce6. amine. The material combines the bactericidal activity of AgNPs with Ce6 photodynamic sterilization, which can effectively improve the aggregation of AgNPs and improve the bactericidal activity of the material. The novel AgNPs‑PEI‑Ce6 nano-antibacterial material can effectively kill planktonic bacteria and remove mature biofilms.

Description

technical field [0001] The invention belongs to the field of biological nanometer antibacterial agents, in particular to an AgNPs-PEI-Ce6 nanometer antibacterial material, a preparation method of the material, and an application of the material. Background technique [0002] Bacterial infections have always been a major health threat to human beings. Before the advent of antibiotics, bacterial infections showed a super lethal rate. The use of antibiotics has greatly reduced the morbidity and mortality of bacterial infections over the past few decades. However, the current drug-resistant bacterial infection caused by the abuse of antibiotics has constituted a new challenge for anti-infective treatment in the new century, and is an important threat to human health today ( Science 2008, 321 , 356). In the natural environment, bacteria usually survive in two different forms: free state (planktonic) and biofilm (Bacterial Biofilm, BF). BF is a common way of bacteria, and more...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K33/38A61K47/59A61P31/04
CPCA61K41/0071A61K33/38A61K47/59A61P31/04A61K2300/00
Inventor 赵灵之彭娟娟周蕴赟孙湃叶玲玥李雨桐
Owner CHINA PHARM UNIV
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