Method for high-throughput identification of interaction between pathogenic bacteria and host molecules

Abstract

The invention relates to the technical field of pathogenic bacteria identification, and discloses a method for high-throughput identification of interaction between pathogenic bacteria and host molecules, which comprises the following steps: identifying genes such as a receptor and some key receptor signals and conduction molecules in a host immune system by using a mode of host cells, knocking out host information molecules to separate out macrophages, infecting macrophages subjected to genome RNA interference transfection by using pathogenic bacteria containing reporter gene plasmids, further knocking out host information molecules by using genes to obtain an infection model, and detecting and analyzing the survival change and function of a host infected with the pathogenic bacteria. By researching an interaction network of pathogenic bacteria protein and host protein and interaction of non-coding RNA and protein, new pathogenic bacteria factors and host factors and a new regulation mechanism are mainly discovered and researched, invasion and survival defect mutant strains are determined, and induced expression of thallus secreted protein in host cells is analyzed in a high-throughput manner.

Description

Technical field [0001] The present invention relates to the field of pathogen identification, in particular a method of identifying the interaction between pathogens and host molecules. Background technique [0002] The pathogenic bacteria produces a pathogenic substance, resulting in a host infection, and the normal flora is in an ecological equilibrium. They do not cause infection of the body, so it belongs to the non-pathogenic fungus. Under certain conditions, because the flora disorder, the host immune function is low or The change in the residential part of the flora has caused an ecological disorder. The normal flora can also cause infection, pathogenic bacteria infection and pathogenic force. The result of the interaction between pathogens - host immune systems, existing pathogens and host molecules The screening efficiency of the method of detecting is low, and the operational error is easily generated. Inventive content [0003] (1) Solving technical issues [0004] In...

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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for high-throughput identification of the interaction between pathogenic bacteria and host molecules, which has the advantages of high-throughput analysis of the induced expression of bacterial secreted proteins in host cells, etc. Solve the problem of low screening efficiency of detection and easy operation error

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