Unlock instant, AI-driven research and patent intelligence for your innovation.

IL-6 in brain-derived small vesicles (NDSEV) as marker for diagnosing and/or treating subarachnoid hemorrhage

A subarachnoid space, IL-6 technology, applied in the field of markers for diagnosis and/or treatment of subarachnoid hemorrhage, can solve problems such as potential ideal biomarkers for the process of pathological changes in patients with SAH that are not found.

Pending Publication Date: 2021-11-02
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Related clinical studies have detected the content of extracellular vesicles in the periphery as biomarkers of cerebral ischemia, including functional proteins and various nucleic acids, but no potential ideal biomarkers that can reflect the pathological changes of SAH patients have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • IL-6 in brain-derived small vesicles (NDSEV) as marker for diagnosing and/or treating subarachnoid hemorrhage
  • IL-6 in brain-derived small vesicles (NDSEV) as marker for diagnosing and/or treating subarachnoid hemorrhage
  • IL-6 in brain-derived small vesicles (NDSEV) as marker for diagnosing and/or treating subarachnoid hemorrhage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, IL-6NDSEV extraction and identification

[0031] 1. Ethics: All patients came from the Neurosurgery Department of the First Affiliated Hospital of Wannan Medical College, and were conducted in accordance with the Declaration of Helsinki. Before inclusion in the study, patients or guardians signed an informed consent. The experiment was approved by the Hospital Ethics Committee.

[0032] 2. Experimental design: In this study, aSAH patients admitted from May 2016 to March 2017 were included. Within 24 hours after the onset of SAH, 117 plasma samples were collected from a total of 224 SAH patients. The mean age standard deviation was 59.91±9.65 years (40-83 years); there were 72 females and 45 males. Exclusion criteria: admission after 72 hours after hemorrhage; non-aneurysmal SAH; patients with liver, kidney, heart or pulmonary insufficiency or infectious diseases; poor prognosis on admission, without any intervention. And age-matched healthy people in fast...

Embodiment 2

[0050] Example 2, IL-6NDSEV as a marker for diagnosis and / or treatment of subarachnoid hemorrhage

[0051] 1. ELISA test, using a specific ELISA kit to detect IL-6 (E-EL-H0102c, Elabscience, Wuhan Yilairite Biotechnology Co., Ltd.) and exosome marker CD81 (CSB-EL004960HU, Cusabio, Wuhan Huamei Bioengineering Co., Ltd.) concentration was quantified. Steps: ① Prepare the standard solution according to the instructions, and then add it to the first two rows of wells, and perform repeated quality control, with two wells for each concentration. Both standard solution and sample solution are 100 μl per well, cover the 96-well plate, and incubate at 37°C for 90 minutes. ② Pour off and dry the liquid in the plate, add 100 μl of biotin-labeled antibody to each well, mix by pipetting, then cover the 96-well plate, and incubate at 37°C for 30 minutes. ③ Shake off the liquid in the plate, add 350 μl of washing solution each, rinse for 2 minutes, pour out and dry the liquid in the plate....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses IL-6 in brain-derived small vesicles (NDSEV) as a marker for diagnosing and / or treating subarachnoid hemorrhage, and IL-6 in NDSEV as a marker for diagnosing and / or treating subarachnoid hemorrhage. The invention also discloses application of the antibody for detecting the IL-6 in the NDSEV in preparation of a kit for diagnosing subarachnoid hemorrhage. The test proves that compared with a normal person, the level of IL-6 in the plasma NDSEV of the aSAH patient is obviously different. The high-level NDSEV IL-6 is in positive correlation with the severity and prognosis of a patient. These results indicate that high levels of neuroinflammatory cascade reactions occur in aSAH patients. IL-6 in NDSEV may be a potential biomarker for predicting disease progression of aSAH patients, and may be used for evaluating prognosis of the disease in the future.

Description

technical field [0001] The present invention relates to IL-6 in NDSEV as a marker for diagnosing and / or treating subarachnoid hemorrhage. Background technique [0002] Aneurysmal subarachnoid hemorrhage (aSAH), usually caused by rupture of aneurysm, is a disease with a high mortality and disability rate, which can occur at any age and accounts for 5-7% of the total incidence of stroke. One of the key prognostic factors is the initial bleeding volume, which can lead to early brain injury (EBI) and early cerebral vasospasm, and is associated with delayed cerebral ischemia (DCI). Recently, more and more researchers have found that early brain injury after SAH may be the main factor affecting the prognosis of SAH patients. Therefore, in the early stage after the occurrence of SAH, it is particularly important to clarify the pathophysiological mechanism of its occurrence, including a series of changes such as microvascular filling defect, ion homeostasis disruption, neuroinflamm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6869G01N2333/5412G01N2800/2871G01N2800/52
Inventor 赖年升姚阳江晓春李真保方兴根吴德刚赵心同夏大勇袁金龙
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE