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Genetically modified clostridium bacteria, preparation and uses of same

A Clostridium bacterium, genetic modification technology, applied in the field of Clostridium bacterium

Pending Publication Date: 2021-11-05
INST FR DU PETROLE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, to date there are no bacterial strains of the genus Clostridium that have been genetically modified to naturally produce isopropanol, in particular to naturally undergo IBE fermentation, in particular genetically modified to be resistant to antibiotics belonging to the amidoalcohol class such as Chloramphenicol or thiamphenicol sensitive, preferably strains capable of optimizing isopropanol production

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  • Genetically modified clostridium bacteria, preparation and uses of same
  • Genetically modified clostridium bacteria, preparation and uses of same
  • Genetically modified clostridium bacteria, preparation and uses of same

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Embodiment Construction

[0018] Despite being used industrially for more than a century, understanding of Clostridium bacteria is still limited by the difficulties encountered in genetically modifying them.

[0019] Different genetic tools have been designed in recent years to optimize strains of this genus, the latest generation being based on the use of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) / CRISPR-Associated Protein (Cas) technology. This method is based on the use of an enzyme called a nuclease (typically a Cas-type nuclease in the case of CRISPR / Cas genetic tools, such as the Cas9 protein from Streptococcus pyogenes), which in the Double-strand cleavage in DNA molecules (target sequences of interest) is guided by RNA molecules. The sequence of the guide RNA (gRNA) determines the cleavage site of the nuclease, endowing it with extremely high specificity ( Figure 17 ).

[0020] Since double-strand cuts in essential DNA molecules are lethal to the organism, the survival...

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Abstract

The present invention concerns the genetic modification of bacteria of the Clostridium genus, typically solventogenic bacteria of the Clostridium genus, in particular bacteria having, in the wild state, a gene coding an amphenicol-O-acetyltransferase. It thus concerns methods, tools and kits allowing such a genetic modification, in particular the elimination or modification of a sequence coding or controlling the transcription of an amphenicol-O-acetyltransferase, the genetically modified bacteria obtained and the uses of same, in particular for producing a solvent, preferably on an industrial scale.

Description

[0001] The present invention relates to the genetic modification of bacteria of the genus Clostridium, generally of the genus Solventogenic Clostridium, in particular bacteria having a gene encoding an amido alcohol-O-acetyltransferase in the wild type. Accordingly, the present invention relates to methods, tools and methods for allowing such genetic modifications, in particular the removal or modification of sequences encoding amidoalcohol-O-acetyltransferases or sequences controlling the transcription of sequences encoding amidoalcohol-O-acetyltransferases. Kits relating to the genetically modified bacteria obtained and to their use, in particular preferably on an industrial scale for the production of solvents. Background technique [0002] The genus Clostridium comprises Gram-positive, strictly anaerobic, spore-forming bacteria belonging to the phylum Firmicutes. Clostridia are an important group to the scientific community for several reasons. The first is that many seri...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12P7/16C12R1/145
CPCC12N15/102C12P7/16C12N9/1033C12Y203/01028C12N15/1137C12N2310/20C12P7/04C12P7/065Y02E50/10C12N15/74C07K14/33C12N15/113C12N9/22C12N2800/80
Inventor 雷米·霍克格拉迪斯·沙尔捷弗朗索瓦·瓦塞尔斯尼古拉斯·洛普斯费雷拉
Owner INST FR DU PETROLE
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