Thrombolytic walnut kernels and preparation method thereof
A technology for walnut kernels and thrombolysis, which is applied in the field of thrombolytic walnut kernels and their preparation, can solve the problems of single product variety of cooked walnut kernels, and achieve the effects of enriching product varieties, improving gastrointestinal function, and improving nutritional value.
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[0026] The preparation method of the bacterial liquid of Bacillus natto is as follows: activate Bacillus subtilis natto in the seed medium, then use a casein plate to screen the colonies with large transparent circles, and pick the screened colonies to expand the cultivation in the seed medium To the bacterial concentration of 2.1 × 10 9 ~2.4×10 9 CFU / mL. The composition of the seed medium is: 5-7g of tryptone, 12-14g of glucose, 2-4g of beef extract, 600mL of distilled water, and the pH is adjusted to 7.0.
[0027] The preparation method of the roasted cooked walnut kernels used in each embodiment is as follows: select raw hickory nuts with plump kernels, dry them in the sun and fry them to remove the astringency, add sand and stones, stir fry them at 200 ° C, and wait for the surface moisture of the walnuts to dry. Evaporate completely, stir-fry with slow fire until the surface of the walnut is ivory white, and crack the shell of the walnut to obtain fried cooked walnut ke...
Embodiment 1
[0029] A preparation method of thrombolytic walnut kernel, comprising the steps of:
[0030] (1) Add 30 mL of distilled water to 20 g of fried cooked walnut kernels, soak at 25°C for 30 minutes, drain the water, and sterilize at 121°C for 30 minutes to prepare a solid-state fermentation substrate for walnut kernels;
[0031] (2) Insert 3mL of Bacillus natto bacteria solution into the solid-state fermentation medium of walnut kernels, and culture it on a shaker at 37°C and 200 r / min for 16 hours;
[0032] (3) pre-freezing the substrate fermented in step (2) at -20°C for 5 hours, and then vacuum freeze-drying at -80°C for 28 hours under a vacuum of 10 Pa;
[0033] (4) Packing the sample in step (3) is the thrombolytic walnut kernel product.
Embodiment 2
[0035] A kind of preparation method of thrombolytic walnut kernel, other is with embodiment 1, just the consumption of the bacterium liquid of bacillus natto is 4mL.
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