Derivative fluorescent probe based on diketopyrrolopyrrole and application thereof
A technology of diketopyrrolopyrrole and fluorescent probe, which is applied in the field of derivatives and its application in the detection of TNP, can solve the problems of long time required for the detection process, high dependence on analytical instruments, complicated preparation work, etc. Achieve the effect of wide detection linear range, sensitive detection effect and good selectivity
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Embodiment 1
[0031]
[0032] Add p-cyanobenzaldehyde (10 g, 76.5 mmol), ethylene glycol (5 mL, 90.5 mmol), p-toluenesulfonic acid (1.25 g, 6.5 mmol) to a round bottom flask containing 25 mL of toluene, and connect The water separator and the condenser tube are reacted at a temperature of 150°C for 2 to 3 hours, and water is removed in time during the reaction. After the reaction was completed, the reaction solution was cooled to room temperature, and 150 mL of saturated aqueous sodium bicarbonate solution was added to the reaction solution for extraction to remove p-toluenesulfonic acid. The organic phase was collected and dried over anhydrous sodium sulfate. Compound A was further refined by silica gel column chromatography, and the eluent was dichloromethane to obtain 11.02 g of compound A with a yield of 82.3%. 1 H-NMR (400MHz, CDCl3) δ: 7.71(d, 2H), 7.65(d, 2H), 5.82(s, 1H), 3.98-4.11(m, 4H). 13 CNMR (100MHz, CDCl3) δ: 142.7, 133.6, 127.3, 118.5, 112.3, 103.6, 65.6.
[0033]
...
Embodiment 2
[0042]
[0043] The synthesis steps of compound C-2 are similar to the synthesis steps of compound C-1 in Example 1, except that the n-bromobutane used in the synthesis of compound C-1 in Example 1 is replaced by bromoethane (2.778mmol ), the synthetic steps of the remaining compounds are the same as in Example 1, and the amount of each compound is also the same as that in Example 1. The NMR data of compound C-2 are as follows: 1 HNMR (400MHz, CDCl 3 )δ: 7.49(d,4H), 7.32(d,4H), 5.80(s,2H), 4.09-3.95(m,8H), 3.75(q,4H), 1.21(t,6H); 13 CNMR (100MHz, CDCl 3 )δ: 163.1, 143.8, 136.7, 135.9, 128.8, 127.5, 106.1, 104.7, 65.7, 38.6, 14.5.
[0044]
[0045] The preparation of compound D-2 was carried out with reference to Example 1, and the NMR data of compound D-2 are as follows: 1 HNMR (400MHz, CDCl 3 )δ: 9.76(s,2H), 7.84(d,4H), 7.58(d,4H), 3.68(q,4H), 1.21(t,6H); 13 CNMR (100MHz, CDCl 3 )δ: 191.4, 166.0, 144.7, 142.4, 135.6, 129.1, 127.6, 106.5, 39.1, 14.5.
[0046] ...
Embodiment 3
[0049]
[0050] The synthesis steps of compound C-3 are similar to the synthesis steps of compound C-1 in Example 1, except that the n-bromobutane used in the synthesis of compound C-1 in Example 1 is replaced by n-bromopropane (2.778mmol ), the synthetic steps of the remaining compounds are the same as in Example 1, and the amount of each compound is also the same as that in Example 1. The NMR data of compound C-3 are as follows: 1 HNMR (400MHz, CDCl 3)δ: 7.38(d,4H), 7.29(d,4H), 5.04(s,2H), 4.21-3.92(m,8H), 3.42(t,4H), 1.50(m,4H), 0.87(t ,6H); 13 CNMR (100MHz, CDCl 3 )δ: 163.1, 140.1, 139.5, 137.4, 129.7, 127.2, 108.1, 104.6, 66.1, 46.1, 21.5, 11.9.
[0051]
[0052] The preparation of compound D-3 was carried out with reference to Example 1, and the NMR data of compound D-3 are as follows: 1 HNMR (400MHz, CDCl 3 )δ: 9.61(s,2H), 7.66(d,4H), 7.41(d,4H), 3.39(t,4H), 1.51(m,4H), 0.77(t,6H); 13 CNMR (100MHz, CDCl 3 )δ: 191.2, 165.5, 144.0, 141.9, 135.7, 129.6, 125.9...
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