Detection method of mint fresh tea fingerprint spectrum
A technology of fingerprints and detection methods, which is applied in the detection field of mint fresh tea fingerprints, can solve the problems of few quality detection methods of mint fresh tea, and achieve the effect of simple method, good reproducibility, and reduced possibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Embodiment 1 A kind of detection method of peppermint refreshing tea fingerprint, comprises the following steps:
[0046] Step 1, the preparation of peppermint fresh tea test solution:
[0047] Take 8 batches of 20g fresh mint tea samples (4g mint, 2g honeysuckle, 2g chrysanthemum, 5g hawthorn, 5g Luo Han Guo, 2g light bamboo leaves) and put them into a 1000mL round bottom bottle after powdering, add methanol solution 400mL, ultrasonically extract for 30min, filter, Take the filtrate and place it in a 500mL volumetric flask, add methanol to constant volume, and pass through a 0.45 μm microporous membrane to obtain the test solution;
[0048] Step 2, preparation of mixed reference solution:
[0049] Take accurately weighed reference substances of chlorogenic acid, luteolin and quercetin, place them in a volumetric flask, dilute to the mark with methanol, shake well, and prepare Grass glycosides, 0.0529mg / ml quercetin mixed reference substance solution.
[0050] Step 3...
Embodiment 2
[0057] Methodology research of embodiment 2 fingerprint spectrum detection
[0058] 1. Methodological investigation
[0059] 1.1 Inspection of precision
[0060] Take mint fresh tea sample 20g (mint 4g, honeysuckle 2g, chrysanthemum 2g, hawthorn 5g, Luo Han Guo 5g, light bamboo leaf 2g), according to the preparation method of the test sample in the above-mentioned embodiment 1, prepare the test solution, and continuously sample for 6 time, each injection volume is 10 μ L, detect according to the chromatographic conditions of the above-mentioned embodiment 1, measure its HPLC chromatogram, investigate 7 common fingerprint peaks in the chromatogram, the results show that the retention time RSD of the common fingerprint peaks is all less than 1.2% , and the common peak area RSDs were all less than 2.4%, indicating that the precision of the instrument was good.
[0061] 1.2 Stability investigation
[0062] Get mint fresh tea sample 20g (mint 4g, honeysuckle 2g, chrysanthemum 2g...
PUM
![No PUM](https://static-eureka.patsnap.com/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka.patsnap.com/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap