Detection method of mint fresh tea fingerprint spectrum

A technology of fingerprints and detection methods, which is applied in the detection field of mint fresh tea fingerprints, can solve the problems of few quality detection methods of mint fresh tea, and achieve the effect of simple method, good reproducibility, and reduced possibility

Active Publication Date: 2021-11-16
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few quality testing methods for fresh mint tea

Method used

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  • Detection method of mint fresh tea fingerprint spectrum
  • Detection method of mint fresh tea fingerprint spectrum
  • Detection method of mint fresh tea fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 A kind of detection method of peppermint refreshing tea fingerprint, comprises the following steps:

[0046] Step 1, the preparation of peppermint fresh tea test solution:

[0047] Take 8 batches of 20g fresh mint tea samples (4g mint, 2g honeysuckle, 2g chrysanthemum, 5g hawthorn, 5g Luo Han Guo, 2g light bamboo leaves) and put them into a 1000mL round bottom bottle after powdering, add methanol solution 400mL, ultrasonically extract for 30min, filter, Take the filtrate and place it in a 500mL volumetric flask, add methanol to constant volume, and pass through a 0.45 μm microporous membrane to obtain the test solution;

[0048] Step 2, preparation of mixed reference solution:

[0049] Take accurately weighed reference substances of chlorogenic acid, luteolin and quercetin, place them in a volumetric flask, dilute to the mark with methanol, shake well, and prepare Grass glycosides, 0.0529mg / ml quercetin mixed reference substance solution.

[0050] Step 3...

Embodiment 2

[0057] Methodology research of embodiment 2 fingerprint spectrum detection

[0058] 1. Methodological investigation

[0059] 1.1 Inspection of precision

[0060] Take mint fresh tea sample 20g (mint 4g, honeysuckle 2g, chrysanthemum 2g, hawthorn 5g, Luo Han Guo 5g, light bamboo leaf 2g), according to the preparation method of the test sample in the above-mentioned embodiment 1, prepare the test solution, and continuously sample for 6 time, each injection volume is 10 μ L, detect according to the chromatographic conditions of the above-mentioned embodiment 1, measure its HPLC chromatogram, investigate 7 common fingerprint peaks in the chromatogram, the results show that the retention time RSD of the common fingerprint peaks is all less than 1.2% , and the common peak area RSDs were all less than 2.4%, indicating that the precision of the instrument was good.

[0061] 1.2 Stability investigation

[0062] Get mint fresh tea sample 20g (mint 4g, honeysuckle 2g, chrysanthemum 2g...

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Abstract

The invention discloses a detection method of a mint fresh tea fingerprint spectrum. The detection method comprises the following steps of: step 1, preparing a mint fresh tea test solution; step 2, preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording chromatograms; step 4, exporting the mint fresh tea fingerprint spectra through an instrument, importing the mint fresh tea fingerprint spectra into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of mint fresh tea as common peaks; generating a contrast fingerprint spectrum of the mint fresh tea by using an average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing the mint fresh tea fingerprint spectra with a mixed standard substance spectrum, and identifying main component peaks. The mint fresh tea fingerprint spectra provided by the method of the invention can comprehensively and objectively characterize the quality of the mint fresh tea. The detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.

Description

technical field [0001] The invention relates to a detection method of a traditional Chinese medicine substitute tea, in particular to a detection method of a mint refreshing tea fingerprint. Background technique [0002] Traditional Chinese medicine substitute tea is a special traditional Chinese medicine formulation for treating diseases, recuperating, improving health and prolonging life, and has played an important role in medical care. Traditional Chinese medicine substitute tea can dialectically formulate prescriptions according to the needs of the disease, add or subtract according to the symptoms, and choose the appropriate method of use according to the performance characteristics of the medicine. The procedure is simple, the deployment is convenient, and the pertinence is strong. The characteristics of flexibility and remarkable curative effect overcome the shortcomings of traditional decoctions such as cumbersome decoction and inconvenient carrying, and adapt to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/02G01N30/06G01N30/14G01N30/34G01N30/74
CPCG01N30/8686G01N30/02G01N30/06G01N30/14G01N30/34G01N30/8634G01N30/8679G01N30/74G01N2030/146Y02A50/30
Inventor 金俊杰李国维刘爱国
Owner NANJING HAICHANG CHINESE MEDICINE GRPCO LTD
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