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SNP marker for identifying apricot peel hair traits, primer and application

A technology for identifying and marking apricots, which is applied in the field of molecular biology, can solve the problems of long cycle of target varieties, achieve low cost, accelerate the breeding process, and shorten the breeding period

Active Publication Date: 2021-11-23
北京市林业果树科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, traditional fruit tree breeding has a certain degree of blindness. On the other hand, it takes a long time to obtain the target variety. It is powerless in the improvement of some complex traits and the joint improvement of multiple traits. It is urgent to use modern molecular biology to promote the breeding process.

Method used

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  • SNP marker for identifying apricot peel hair traits, primer and application
  • SNP marker for identifying apricot peel hair traits, primer and application
  • SNP marker for identifying apricot peel hair traits, primer and application

Examples

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Embodiment 1

[0049] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular Cloning Experiment Manual (Sambrook J & Russell DW, Molecular Cloning: a Laboratory Manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions. Example 1 Using 46 Li Guangxing fruit offspring to verify the hairy / glabrous traits of the fruit epidermis

[0050] Implementation time: 2020;

[0051]Implementation site: Beijing Forestry and Fruit Tree Science Research Institute;

Embodiment approach

[0053] The population of seed progeny of Li Guangxing was selected, which was a half-sib family. The total DNA of 46 leaves of seedlings of Li Guangxing was extracted by CTAB method, and the purity of DNA was identified. Then use primers (SEQ ID NO:2-3), and carry out PCR reaction according to the above-mentioned steps, after gel electrophoresis of the obtained PCR amplification product, use common agarose gel DA recovery reagent to recover the target fragment, and carry out a generation Sequencing was performed to detect the genotype of the polymorphic site corresponding to the SNP marker of the PCR product, and the results are shown in Table 3.

[0054] Table 3 Verification results of hairy / glabrous epidermis of 46 Li Guangxing apricot offspring

[0055]

Embodiment 2

[0056] Example 2 Select 244 apricot germplasm resources to verify the hairy / hairless traits of the fruit epidermis

[0057] Implementation time: 2020;

[0058] Implementation site: Beijing Forestry and Fruit Tree Science Research Institute;

[0059] implementation plan:

[0060] (1) Total DNA was extracted from leaves of 244 apricot germplasm resources by CTAB method, and the purity of DNA was identified.

[0061] (2) Synthesize primers (SEQ ID NO:2-3), and carry out PCR reaction according to the above steps to obtain PCR amplification products. Sequencing, and then detecting the genotype of the polymorphic site corresponding to the SNP marker of the PCR product.

[0062] (3) Detection of the correlation between SNP molecular markers and hairy and hairless traits

[0063] Use the above primers to verify the reliability of 244 pairs of SNP molecular markers. The genotypes GG and GC are hairy in the apricot fruit epidermis, and CC is hairless. The fruit epidermis of all apri...

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Abstract

The invention provides an SNP marker for identifying apricot peel hair traits, a primer and application. According to the SNP marker related to the hairy / hair-free character of the apricot fruit epidermis, the SNP marker related to the hairy / hair-free character of the apricot fruit epidermis is obtained through a whole genome association analysis method, the polymorphic site is located at the 15954845bp position of the No. 7 chromosome of an apricot genome and is a basic group G or C, and a detection primer is designed aiming at the site; according to the method, one pair of primers is utilized, whether apricot fruit epidermis is hairy or not can be identified in the seedling stage, early screening of hairy / hair-free characters is achieved, the breeding age limit is shortened, through verification of 244 parts of apricot resources, the result shows that the identification accuracy of the method on the hairy / hair-free character of the surface skin of the apricot fruit reaches 100%; and the method the hairy / hair-free character of the apricot fruit epidermis has the advantages of simplicity, rapidness, high efficiency, low cost and the like, and has an important application value in fresh apricot breeding.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a SNP marker, a primer and an application for identifying the fur traits of apricot fruit. Background technique [0002] Since the 1970s, my country began to select apricot seedlings and farm varieties, and in the 1980s, purposeful cross breeding began, providing a large number of suitable varieties for production. However, on the one hand, traditional fruit tree breeding has a certain degree of blindness. On the other hand, it takes a long time to obtain target varieties. It is powerless in the improvement of some complex traits and the joint improvement of multiple traits. It is urgent to use modern molecular biology methods to promote the breeding process. [0003] Molecular marker-assisted breeding is a technology developed rapidly in recent years, which provides new ideas and approaches for breeding work. SNP refers to the change of 1 nucleotide in the genome of different v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13Y02P60/87
Inventor 张美玲姜凤超孙浩元杨丽张俊环王玉柱
Owner 北京市林业果树科学研究院
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