Method for detecting target breast milk oligosaccharide in formula food

A technology for breast milk oligosaccharides and formula food, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as increasing the difficulty of separation, and achieve the effects of increasing detection sensitivity, accurate qualitative and quantitative, and removing interfering impurities.
CN113686992AActive Publication Date: 2021-11-23HEILONGJIANG FEIHE DAIRY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
HEILONGJIANG FEIHE DAIRY
Publication Date
2021-11-23

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Abstract

The invention relates to a method for detecting target breast milk oligosaccharide in formula food. The target breast milk oligosaccharide may include or be one or more of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, lactose-difucosyltetraose, lactose-N-neotetraose, and lactose-N-tetraose. The method comprises the following steps: removing fat and protein from a sample, reducing by adopting a reducing agent such as NaBH4, detecting by adopting liquid chromatography-mass spectrometry, and quantifying by adopting an external standard method. According to the method, the target breast milk oligosaccharide (HMO) can be simply, conveniently, quickly, accurately, qualitatively and quantitatively detected.
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Description

technical field

[0001] The invention relates to the field of food. Specifically, the present invention relates to a method for detecting target breast milk oligosaccharides in formula food. Background technique

[0002] Breast milk is the most natural and high-quality food for newborns. Breast milk contains various nutrients, such as protein, fat, carbohydrates, inorganic salts and trace elements. Their reasonable ratio is suitable for infants to digest and absorb. In addition, breast milk also contains biological activity Substances that have short-term anti-infective benefits.

[0003] Human milk oligosaccharides (HMO) are the third largest solid component in human breast milk, second only to lactose and fat. Human milk oligosaccharides are free polysaccharides composed of 3-22 monosaccharide units. According to the presence or absence of sialic acid, HMOs can be divided into two categories: neutral HMOs and acidic HMOs. The reducing ends of HMOs all contain lactose. ...

Claims

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