Detection method of astaxanthin in Haematococcus pluvialis based on snapshot multispectral imaging
A technology of Haematococcus pluvialis and multispectral imaging, which is applied in the field of detection of astaxanthin content of Haematococcus pluvialis, can solve the problems of time-consuming, complicated operation of detection methods, etc., and achieves small volume, fast sampling and data processing speed. Effect
Active Publication Date: 2022-06-21
FUZHOU UNIV
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[0004] The object of the present invention is to provide a method for detecting astaxanthin content in Haematococcus pluvialls based on snapshot multispectral imaging, and establish an astaxanthin content prediction model through snapshot multispectral imaging technology combined with chemometrics methods, and combine the image information, realize monitoring visualization, and solve the problems of relatively cumbersome and time-consuming operation of existing detection methods
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[0031] The technical solutions of the present invention will be described in detail below with reference to the accompanying drawings.
[0032] A method for detecting astaxanthin content of Haematococcus pluvialis based on snapshot multi-spectral imaging of the present invention comprises the following steps:
[0033] (1) Take the samples of Haematococcus pluvialis in the exponential growth phase, prepare algal liquid samples with different concentration gradients, and divide them into two groups, one group is used for the detection of astaxanthin content by high performance liquid chromatography, and the other group is used for the detection of astaxanthin content. Collection of reflection spectra;
[0034] (2) Collect the spectral reflectance value of the algal fluid sample under the snapshot multi-spectral imaging system; measure the astaxanthin content corresponding to the algal fluid sample;
[0035] (3) Select 1000-2000 pixels of the algal fluid sample as the region of ...
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The invention relates to a method for detecting astaxanthin content of Haematococcus pluvialis based on snapshot multispectral imaging. It includes the following steps: 1) Construct a snapshot multispectral reflectance imaging system, which is composed of a snapshot multispectral camera, a wide spectrum light source, a stage, a sample pool, a bracket, a hood, etc.; 2) Make rain at different growth stages and concentrations Haematococcus pluvialis sample set, standardize the biomass and astaxanthin content of the sample set through traditional detection methods; 3) Collect spectral images of Haematococcus pluvialis samples, perform spectral preprocessing and sample set division; 4) Establish a prediction model; 5 ) to build a sample visualization model. The invention can perform rapid and non-destructive detection of Haematococcus pluvialis astaxanthin within the concentration range of 0.3-3.0 g / L in the visible light band 470-640nm, and realize dynamic monitoring of Haematococcus pluvialls astaxanthin in industrial production.
Description
technical field [0001] The invention relates to the technical field of microalgae pigment content detection, in particular to a method for detecting astaxanthin content of Haematococcus pluvialis based on snapshot multispectral imaging. Background technique [0002] Astaxanthin is the highest-level product in carotenoid biosynthesis, with the strongest antioxidant, anti-cancer, enhancement of immunity, prevention of cardiovascular disease and other physiological activities. The content of Haematococcus pluvialis astaxanthin in algae is as high as 5% of the dry weight. Haematococcus pluvialis has a broad application prospect in the production of astaxanthin. The traditional detection methods of Haematococcus pluvialis astaxanthin content are generally through ultrasonic extraction, microwave extraction, CO 2 Extraction by supercritical extraction, etc., and then measurement by spectrophotometry, high performance liquid chromatography and liquid chromatography with mass spect...
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IPC IPC(8): G01N21/25
CPCG01N21/25G01N2021/1765
Inventor 沈英陈海涛黄春红黄峰
Owner FUZHOU UNIV