Method for detecting content of caffeine in tea leaves

A detection method and caffeine technology, which are applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of low detection accuracy and inability to accurately reflect the caffeine content in tea leaves, and achieve high detection accuracy, efficient adsorption, and improved detection accuracy. Effect

Active Publication Date: 2021-12-03
SHAOYANG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very necessary to detect the content of caffeine in tea, but the existing detection methods have the defect of low detection accuracy and cannot accur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] 1. Preparation of Nanoadsorbent Materials

[0031] FeCl 3 ·6H 2 O (1g), PEG-800 (0.6g) and trisodium citrate (1g) were ultrasonically dispersed in 100mL of ethylene glycol, then 4g of anhydrous sodium sulfate was added, the solution was stirred until clear, and then the solution was reacted at 200°C 8h, centrifugation, drying, and soaking the obtained nanomaterial in 200 mL of butyric acid for 15 minutes to obtain a nanoadsorbent material.

[0032] 2. Detection of caffeine content in tea

[0033] (1) 10g of tea leaves were pulverized and added to 500mL of ethanol to reflux for 1h, and the reflux liquid was concentrated and freeze-dried to obtain 2.05g of powdery solids;

[0034] (2) ultrasonically treating the powdery solid obtained in step 1 in an aqueous solution for 5min, filtering, adding 4.10g of nano-adsorbent material to the filtrate, ultrasonically treating for 2min, and collecting nano-adsorbent material after centrifugation;

[0035] (3...

Example Embodiment

[0038] Example 2

[0039] 1. Preparation of Nanoadsorbent Materials

[0040] FeCl 3 ·6H 2 O (1.1g), PEG-800 (0.7g) and trisodium citrate (1.2g) were ultrasonically dispersed in 120mL of ethylene glycol, then 6g of anhydrous sodium sulfate was added, the solution was stirred until clear, and then the solution was heated at 205°C The reaction was carried out for 8.5 hours, centrifuged and dried, and the obtained nanomaterials were soaked and adsorbed in 200 mL of butyric acid for 10 minutes to obtain nanometer adsorption materials.

[0041] 2. Detection of caffeine content in tea

[0042] (1) 10g of tea leaves were pulverized and added to 500mL of ethanol to reflux for 1.5h, and the reflux liquid was concentrated and freeze-dried to obtain 2.04g of powdery solid;

[0043] (2) ultrasonically treating the powdery solid obtained in the step (1) in an aqueous solution for 10 min, filtering, adding 4.04 g of nano-adsorbent material to the filtrate, ultrasonically treating for 3 m...

Example Embodiment

[0047]Example 3

[0048] 1. Preparation of Nanoadsorbent Materials

[0049] FeCl 3 ·6H 2 O (1g), PEG-800 (0.7g) and trisodium citrate (1g) were ultrasonically dispersed in 100mL of ethylene glycol, then 5g of anhydrous sodium sulfate was added, the solution was stirred until clear, and then the solution was reacted at 200°C After 8.3 hours, centrifugation and drying, the obtained nanomaterials were soaked and adsorbed in 200 mL of butyric acid for 12 minutes to obtain nanoadsorbent materials.

[0050] 2. Detection of caffeine content in tea

[0051] (1) 10g of tea leaves were pulverized and added to 500mL of ethanol to reflux for 1h, and the reflux liquid was concentrated and freeze-dried to obtain 2.02g of powdery solids;

[0052] (2) ultrasonically treating the powdery solid obtained in step 1 in an aqueous solution for 8 min, filtering, adding 3 g of nano-adsorbing material to the filtrate, ultrasonically treating for 3 min, and collecting nano-adsorbing material after ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detecting the content of caffeine in tea leaves, and relates to the technical field of food detection. The method specifically comprises the following steps: carrying out ultrasonic extraction on a product obtained by refluxing tea leaves with ethanol, then adsorbing with a nano adsorption material, eluting the adsorbed nano adsorption material, concentrating and filtering eluent, and carrying out liquid chromatography detection. On the basis of the adsorption characteristic of the nano-material, butyric acid is further adsorbed in the pore structure of the nano-material, and high-efficiency adsorption of free caffeine is comprehensively realized by virtue of relatively strong intermolecular force between butyric acid and caffeine; in the subsequent analysis and detection, the mobile phase components and the elution procedure are specifically set, so that the final caffeine detection precision can reach 99.96%.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting caffeine content in tea. Background technique [0002] Caffeine, also known as caffeine, its chemical name is 1,3,7-trimethyl-3,7-dihydro-1H-purine-2,6-dione hydrate, which is a xanthine alkaloid compound belonging to A central nervous system stimulant that can temporarily drive away drowsiness and restore energy. [0003] Tea is the best natural health care product for human beings. Tea contains more than 450 kinds of organic compounds, more than 15 kinds of inorganic minerals and essential nutrients and medicinal ingredients for human body. Tea is effective in the prevention and treatment of arteriosclerosis, hypertension, atherosclerosis, radiation damage, tumors, and decreased immune function. Drinking tea has the functions of health preservation, health care, and cancer prevention, which cannot be replaced by other beverages. [0004] Tea cont...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 傅春燕袁治倩刘永辉曾伟
Owner SHAOYANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap