Method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri and application of method

A technology of Cortex Phellodendron and Coptis chinensis, applied in the field of simultaneous identification of Coptis chinensis and Phellodendron phellodendron, can solve problems such as identification of phellodendron cortex, achieve high accuracy, save time and cost, and achieve uniform quality

Active Publication Date: 2021-12-07
BEIJING KANGRENTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot identify Phellodendron Phellodendron in the prescription, and Phellodendron Phellodendron is one of the important raw materials of Danggui Liuhuang Decoction

Method used

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  • Method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri and application of method
  • Method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri and application of method
  • Method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri and application of method

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preparation example Construction

[0089] (1) Preparation of Danggui Liuhuang Decoction: According to the mass ratio of Danggui, Rehmannia glutinosa, Rehmannia glutinosa, Scutellaria baicalensis, Coptis chinensis, Cortex Phellodendron and Astragalus membranaceus in a mass ratio of 1:1:1:1:1:1:1:2, weigh Danggui, rehmannia glutinosa, rehmannia glutinosa, Rehmannia glutinosa, Scutellaria baicalensis, Coptis chinensis, Cortex Phellodendron, and Astragalus membranaceus, mixed, crushed into coarse powder, added 20 times the amount of water (the amount refers to the number of milliliters of water added to each gram of medicinal materials), and decocted until the volume of the medicinal liquid is half of the volume of water added , filtered to obtain Angelica Liuhuang Decoction.

[0090] (2) Preparation of Danggui Liuhuang Decoction substance reference material: Danggui Liuhuang Decoction was prepared according to the above method, dried to obtain Danggui Liuhuang Decoction substance reference substance powder.

[009...

Embodiment 1

[0097] The present embodiment provides a method for simultaneously identifying components of Coptis Rhizoma and Phellodendron Phellodendri, comprising the following steps:

[0098] (1) Preparation of solution

[0099] Preparation of the test solution: Take 2 g of the reference material powder of Danggui Liuhuang Tang, add 20 ml of methanol, ultrasonically treat for 30 minutes, filter, and the filtrate is used as the test solution.

[0100] Preparation of reference medicinal solution: Take 2 g of Coptidis Rhizoma and Phellodendron Phellodendri as reference medicinal materials, add 20 ml of methanol respectively, ultrasonically treat for 30 minutes, and filter to prepare Coptis Rhizoma and Phellodendron Phellodendron reference medicinal solution respectively.

[0101] Negative control solution preparation: take Coptidis single-negative test powder, Phellodendron single-negative test powder, and Coptidis Phellodendron double-negative test powder 2g, respectively add 20ml of metha...

Embodiment 2

[0108] The present embodiment provides a method for simultaneously identifying components of Coptis Rhizoma and Phellodendron Phellodendri, comprising the following steps:

[0109](1) Preparation of solution

[0110] Preparation of the test solution: take Angelica Liuhuang Decoction, dry it to obtain a powder, take 2 g of the powder and add 20 ml of methanol, sonicate for 20 minutes, filter, and the filtrate is used as the test solution.

[0111] Preparation of the reference medicinal material solution: take 2 g of the reference medicinal materials Coptidis Rhizoma and Phellodendron Phellodendri, and prepare the reference medicinal material solution according to the above-mentioned "preparation of the test sample solution".

[0112] Preparation of negative control solution: Take 1 g of Coptidis single-negative, Phellodendron single-negative test powder, and Coptis Phellodendron double-negative test powder, and prepare a negative control solution according to the above-mentione...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly provides a method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri, which comprises the following steps: (1) preparing a test solution, a reference solution and a reference medicinal material solution; (2) thin layer chromatography detection: carrying out two-time expansion and respective inspection, wherein butyl acetate-methanol-isopropanol-water is adopted as a developing solvent for first-time developing, n-butanol-water-acetic acid is adopted as a developing solvent for second-time developing, a'secondary thin-layer developing method ' is utilized for the first time, the developing solvent with specific composition is adopted, the same thin-layer plate is adopted, and second-time sample application is not needed; the method can be used for simultaneously and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, avoids the increase of resources and cost caused by independently and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, ensures the uniformity, stability and reliability of quality, and has the advantages of high accuracy, time and cost saving and easiness in popularization and application.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicines, and in particular relates to a method for simultaneously identifying components of Coptis Rhizoma and Phellodendron Phellodendri and its application. Background technique [0002] With the enhancement of people's awareness of health care, there are higher requirements for the quality and safety of Chinese patent medicine products. Danggui Liuhuang Tang is a famous recipe created by Li Dongyuan, one of the four masters of the Jin and Yuan Dynasties, and it is contained in the book "The Secret Collection of Lanshi" written by him. It is called "the holy medicine for night sweats", and it is mainly used to treat night sweats caused by yin deficiency and hyperactivity of fire. The Danggui Liuhuang Decoction is composed of 7 herbs: Angelica, Rehmannia, Rehmannia, Coptidis, Scutellaria, Phellodendron, and Astragalus. [0003] At present, there is no standard promulg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95Y02A50/30
Inventor 张志强韩妮娜韩红梅邓海霞梁勇满刘利娟李纳纳付静
Owner BEIJING KANGRENTANG PHARMA
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