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Application of marker in diagnosis of abdominal aortic aneurysm

An abdominal aortic aneurysm, biomarker technology, applied in the field of biomedicine

Active Publication Date: 2021-12-14
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no drug available clinically for the treatment of AAA

Method used

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  • Application of marker in diagnosis of abdominal aortic aneurysm
  • Application of marker in diagnosis of abdominal aortic aneurysm
  • Application of marker in diagnosis of abdominal aortic aneurysm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1. Patient's Abdomen CT (Computed Tomography) Examination

[0052] All subjects underwent abdominal CT examination to determine whether they had abdominal aortic aneurysm. The inspection results are as follows:

[0053] 1. CT imaging changes

[0054] For patients with abdominal aortic aneurysm ( figure 1 A) and control ( figure 1 B) CT examination of the abdomen, the results showed that the abdominal aorta was obviously dilated in a patient with abdominal aortic aneurysm.

Embodiment 2

[0055] Example 2. Blood collection and analysis of patients with abdominal aortic aneurysm

[0056] 1. Experimental purpose

[0057] Plasma samples from patients with abdominal aortic aneurysm were obtained; protein expression in control plasma was compared. Controls were healthy subjects without abdominal aortic aneurysm.

[0058] 2. Materials and Reagents

[0059] (1) Protein chip:

[0060] The GSH-CAA-440 chip of American RayBiotech Company was used to detect 440 protein factors in the subjects' blood.

[0061] 3. Experimental method

[0062] (1) Sample collection process: Blood samples from abdominal aortic aneurysm patients and controls were collected using EDTA anticoagulated blood collection tubes, centrifuged at 2 000 g for 15 min at 4°C, and the supernatant was drawn and stored in a -80°C refrigerator.

[0063] (2) Experimental process: After the antibody chip was blocked at room temperature for 1 hour, 60 μL of three-fold diluted plasma was added to each well, and...

Embodiment 3

[0070] Example 3. Application of Blood Protein Markers in AAA Diagnosis

[0071] In order to further confirm the sensitivity and specificity of the screened protein markers in the diagnosis of AAA, for the proteins in Table 1, using commercially available antibodies, the present invention draws the ROC of the above markers in distinguishing abdominal aortic aneurysm patients and healthy controls curve. The specific steps are as follows: plasma samples of 20 patients with abdominal aortic aneurysm and 10 healthy control subjects were collected, the two groups were matched in gender and age, blinded, and the protein expression level in the samples was tested by ELISA method. According to ROC curve analysis, the AUC value of human pod protein in Table 1 is 0.861, the sensitivity is 68.18%, and the specificity is 90%; the AUC value of receptor tyrosine protein kinase erbB-3 is 0.832, the sensitivity is 54.55%, The specificity was 100%; the AUC value of Delta-like protein 1 was 0....

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Abstract

The invention discloses application of a marker in diagnosis of abdominal aortic aneurysm. The marker comprises one or more of LGMN, ERBB3 and DLL1. The method for diagnosing abdominal aortic aneurysm is simple and convenient, diagnosis can be completed by extracting a small amount of blood of a subject, pain of the subject is reduced, and the detection result is accurate and reliable.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of markers in the diagnosis of abdominal aortic aneurysm. Background technique [0002] Abdominal aortic aneurysm (AAA) is a common life-threatening arterial degenerative disease, which is defined as the continuous expansion of the three-layer structure of the arterial wall in the abdominal aortic segment to 1.5 of the diameter of the abdominal aorta at the renal artery. more than double. AAA is a common disease in the elderly, and the incidence of AAA in men over 65 years old in Western countries is about 5-10%. The main complication of AAA is rupture. Once ruptured, the mortality rate can reach more than 90%, and about 200,000 people die each year worldwide. However, AAAs are usually asymptomatic until they rupture. [0003] Large-sample studies have found that screening for AAA using abdominal ultrasonography in men over 65 years of age can reduce AAA-related ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/573G01N33/68
CPCC12Q1/6886G01N33/57407G01N33/57484G01N33/573G01N33/68C12Q2600/158C12Q2600/136G01N2333/96466G01N2333/912G01N2333/47
Inventor 吴建强王威郑月宏
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI