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Application of salt-tolerant aerobic denitrifying bacteria and coupled activated carbon thereof in strengthening water body pollution treatment

A water pollution, porous activated carbon technology, applied in the field of microorganisms, can solve problems such as unclear effect of strengthening water pollution control, and yet to strengthen water body, pollution control and other problems.

Active Publication Date: 2021-12-17
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the resources of salt-tolerant aerobic denitrifying bacteria that have been isolated and obtained are very few, and the effect of salt-tolerant aerobic denitrifying bacteria coupled with activated carbon to strengthen water pollution control is still unclear.
[0004] Zobellella sp. is a Gram-negative bacterium, and it has been found that this kind of microorganism has the function of salt-tolerant aerobic denitrification, but there is no relevant report about this kind of microorganism coupled with activated carbon to strengthen the treatment of water pollution

Method used

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  • Application of salt-tolerant aerobic denitrifying bacteria and coupled activated carbon thereof in strengthening water body pollution treatment
  • Application of salt-tolerant aerobic denitrifying bacteria and coupled activated carbon thereof in strengthening water body pollution treatment
  • Application of salt-tolerant aerobic denitrifying bacteria and coupled activated carbon thereof in strengthening water body pollution treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and identification of Zobellella sp. Y61

[0033] Add 50 mL of enrichment medium (KNO 3 1.0g, MgSO 4 3.0g, MgCl 2 2.0g, CaCl 2 1.0g, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.1g, NaHCO 30.2g, KCl 0.7g, NaCl 15.0g, sodium succinate 1.0g, deionized water 1L, pH7.5, add 20g / L agar to the solid medium, sterilize and prepare for later use), shake at 30°C at 160rpm / cultured under the condition of min. After culturing for 5 days, transfer to a new enriched medium according to 10% of the inoculum amount, and then put it in a shaker to continue culturing. The same transfer operation is repeated 4 times. Spread the enriched culture on the solid medium by diluting the plate, place the coated plate in an incubator at 30°C and incubate it upside down for 2-3 days, pick the grown single colony and streak, and repeat Repeat streaking until purified to pure bacteria.

[0034] Inoculate the isolated strains into the enrichment medium, culture in a shaker at 3...

Embodiment 2

[0038] Example 2: Denitrification function of Zobellella sp. Y61 under aerobic conditions

[0039] Configure liquid medium (sucrose 2.0g, sodium pyruvate 2.0g, KNO 3 1.0g, KCl 1.0g, MgSO 4 ·7H 2 O1.0g, K 2 HPO 4 0.5g, NaCl 15.0g, deionized water 1L, pH7.5), sterilized and cooled to room temperature, and aseptically packed 200mL medium into 300mL Erlenmeyer flasks, the experiment was divided into two groups: (1) with Bacteria experimental group, culture Zobelella Y61 in advance to the logarithmic phase, take 100 μL and inoculate it into the sterilized liquid medium, mix well; (2) Aseptic control group, do not inoculate Zobelella Sterilized liquid medium of Y61 strain. All Erlenmeyer flasks were cultured on a shaker at 30°C and 60r / min. The experimental group and the control group were set up in 3 parallels, respectively at 0 days, 0.5 days, 1 day, 1.5 days, 2 days, 3 days and 4 days. Samples were taken to measure the concentration changes of TN, TC and TOC, and the aero...

Embodiment 3

[0041] Example 3: Denitrification function of Zobellella sp. Y61 coupling fruit shell activated carbon under aerobic conditions

[0042] Configure liquid medium (sucrose 1.0g, KNO 3 1.0g, KCl 1.0g, MgSO 4 ·7H 2 O 1.0g, K 2 HPO 4 0.5g, NaCl 15.0g, deionized water 1L, pH7.5), fully stirred and dissolved, then distributed 200mL medium into 300mL Erlenmeyer flasks, added 0.5% medium quality shell activated carbon (10-24 mesh) to the culture medium The medium was used after sterilization, and the experiment was divided into two groups under sterile conditions: (1) The experiment group with bacteria, cultured Zopeia spp. Y61 to the logarithmic phase in advance, and inoculated 100 μL into the sterilized liquid medium, mixed evenly; (2) aseptic control group, a liquid medium in which the sterilized treatment of the Zobella Y61 strain was not inoculated and the fruit shell activated carbon was added. All Erlenmeyer flasks were cultured at 0, 60, 120 and 180 r / min on a shaker at 3...

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Abstract

The invention discloses an application of salt-tolerant aerobic denitrifying bacteria and coupled activated carbon thereof in strengthening water body pollution treatment. The invention relates to Zobellella sp.Y61, and the preservation number of the Zobellala sp.Y61 is GDMCC NO:61848. The Zobellella sp.Y61 has a salt-tolerant aerobic denitrification function, the removal rate of total nitrogen and organic matters in a water body can be increased by coupling the Zobellella sp.Y61 with shell activated carbon (10-24 meshes), and a new method is provided for purifying the polluted water body.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of Zobellella sp. Y61 with a salt-tolerant aerobic denitrification function and coupled activated carbon in strengthening water body pollution control. Background technique [0002] Effective purification of polluted water bodies is an important prerequisite for protecting water ecological safety and health. Since the nitrogen cycle is closely coupled with the cycle of various substances, it is also an important factor determining the degree of eutrophication in water bodies, so the removal effect of nitrogen has attracted much attention. The traditional biological denitrification process is to first oxidize ammonia nitrogen to nitrate through nitrification reaction under aerobic conditions, and then reduce nitrate to gaseous nitrogen through denitrification reaction under anoxic conditions to remove it from water. The denitrification efficiency of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/02C02F101/16C02F101/30C02F103/20
CPCC02F3/02C02F3/34C02F2101/163C02F2103/20C02F2305/06C02F2101/30Y02W10/10
Inventor 许玫英郭俊梅承芳
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY