Culture method for improving bacteriostatic activity of lactobacillus plantarum
A technology of Lactobacillus plantarum and bacteriostatic activity is applied in the cultivation field of improving the bacteriostatic activity of Lactobacillus plantarum, which can solve the problems of insufficient utilization of antiseptic and bacteriostatic functions, less exploitation of natural preservatives, human body influence, etc. and utilization, enhancing the production of bacteriostatic active substances and the ability of bacteriostatic activity, and improving the effect of bacteriostatic activity
Inactive Publication Date: 2021-12-21
WUHU INST OF TECH
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Problems solved by technology
[0002] At present, synthetic preservatives are widely used in food, but a large amount of use will affect the human body, and there is less development of natural preservatives
Lactobacillus can produce a variety of antibacterial substances, but its antiseptic and antibacterial functions are underutilized
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[0018] The technical solution of the present invention will be further described in detail below.
[0019] 1. Optimization of culture conditions
[0020] 1. Effects of different culture temperatures on the production of antibacterial active substances
[0021] It can be seen from Table 1 that when the culture temperature is 35 °C, the growth of the bacteria is the best, and the OD 600 The value reached 2.76, but as the temperature increased, the OD 600 The value tends to rise first and then fall. However, the diameter of the maximum inhibition zone was the largest at 30°C, reaching 25.82mm. Analysis of variance showed that there were significant differences among the groups. Considering the antibacterial activity, it was better at 30°C than at 35°C. Considering relevant indicators comprehensively, 30°C was selected as the optimal culture temperature.
[0022] Table 1 Effect of culture temperature on antibacterial activity of strains
[0023]
[0024] 2. Effect of init...
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Abstract
The invention discloses a culture method for improving the bacteriostatic activity of lactobacillus plantarum. The invention further researches and develops the property of a bacterial strain which is identified as lactobacillus plantarum for producing bacteriostatic active substances, and the production of the bacteriostatic active substances has great correlation with a fermentation process, a culture medium formula and the like except for the genetic factors of the bacterial strain. According to the method, a plurality of influence factors such as culture temperature, culture medium initial pH value, culture medium carbon source, nitrogen source, inorganic ions, stimulating factors and the like are optimized, so that the yield of bacteriostatic active substances is increased.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cultivation method for improving the bacteriostatic activity of plant lactobacillus. Background technique [0002] At present, synthetic preservatives are widely used in food, but a large amount of use will affect the human body, and there is less development of natural preservatives. Lactobacillus can produce a variety of antibacterial substances, but its antiseptic and antibacterial functions are underutilized. Based on the relevant research reports on the antibacterial effect and antibacterial experiments on lactic acid bacteria such as Lactobacillus plantarum, it has also begun to attract attention as a new type of natural preservative. The technology of single factor orthogonal experiment on microbial culture conditions is also relatively mature. Contents of the invention [0003] The invention optimizes the culture conditions and fermentation environment of th...
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IPC IPC(8): C12N1/38C12N1/20C12R1/25
CPCC12N1/38C12N1/20
Inventor 洪伟孙梅王慧敏李子健薛正莲张冬冬
Owner WUHU INST OF TECH