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Akkermansia muciniphila and application thereof to preparation of antitumor drug

A mucin and tumor technology, applied in the field of biopharmaceuticals, can solve the problems of long preparation cycle, large side effects and high cost of anti-tumor drugs

Pending Publication Date: 2021-12-31
JIANGXI PRECISION GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a strain of Akkermansia muciniphila (Akkermansia muciniphila SZ) and its preparation, diagnosis, prevention and / or treatment of tumors in order to solve the problems of large side effects, long preparation period and high cost of existing antitumor drugs. applications in products or medicines

Method used

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  • Akkermansia muciniphila and application thereof to preparation of antitumor drug
  • Akkermansia muciniphila and application thereof to preparation of antitumor drug
  • Akkermansia muciniphila and application thereof to preparation of antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Akkermansia muciniphila CCTCC.M.2020950

[0080]It is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC.M.2020950, the preservation date is December 21, 2020, and the preservation address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

Embodiment 2

[0081] Example 2 Cultivation of Akkermansia muciniphila CCTCC.M.2020950

[0082] Liquid medium: BHI (38.5g / L) + mucin (2g / L), of which BHI medium components (g / L) are: tryptone 10g, beef heart extract powder 17.5g, sodium chloride 5g, glucose 2g , Disodium hydrogen phosphate (12H 2 O) 2.5g.

[0083] Culture conditions: strict anaerobic culture at 37°C; inoculum size 1:100, culture for 72-96 hours.

[0084] Growth curve see attached image 3 .

Embodiment 3

[0085] Example 3 Viable bacteria of Akkermansia muciniphila CCTCC.M.2020950

[0086] Get 100 mL of culture obtained in Example 2 (8000 rpm, 20 min) to collect the thalline by high-speed centrifugation, add PBS containing 2.5% glycerol for suspension, and adjust the concentration of viable bacteria to about 5 × 10 8 CFU / mL.

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Abstract

The invention provides Akkermansia muciniphila and an application thereof to preparation of an antitumor drug, and relates to the technical field of biological pharmacy. The Akkermansia muciniphila is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC. M.2020950, the preservation date is December 21, 2020, and the preservation address is No. 299 on eight road, Wuchang District, Wuhan, Hubei Province. The application refers to the application to preparation of the antitumor drug. A new strain Akkermansia muciniphila SZ of the Akkermansia muciniphila is obtained through separation, culture and identification, fermentation supernate and viable bacteria obtained through in-vitro fermentation of the strain have the effect of remarkably inhibiting tumor cell proliferation, the strain can be used for preparing products or drugs for diagnosing, preventing and / or treating tumors, the preparation cost is low, the preparation efficiency is high, the side effect is small, and good industrial application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a strain of Akkermansia muciniphila and its application in the preparation of antitumor drugs. Background technique [0002] Colon cancer is one of the most common malignant tumors of the digestive system, second only to gastric cancer and esophageal cancer in incidence, and seriously affects human life and health. The curative effect of metastatic colon cancer is poor, and the recurrence rate after surgery is high. The 5-year survival rate of patients with unresectable tumors is almost zero. Although drug-targeted therapy has achieved great success, there are still many shortcomings, such as the large molecular weight of the antibody used for targeting, low penetration in tumor tissue, and toxic effects on the liver and bone marrow; strong immunogenicity; The preparation process and period are long, and the price is expensive. Therefore, the research and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C07K14/195A61K35/741A61P35/00A61P1/00A61P1/16A61P1/18C12R1/01
CPCC12N1/20C07K14/195A61K35/741A61P35/00A61P1/00A61P1/16A61P1/18Y02A50/30
Inventor 朱永亮刘丹穆晓静朱蒙蒙马梦楠马泽为徐青青
Owner JIANGXI PRECISION GENE CO LTD
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