Application of carbonic anhydrase 1 and acid sphingomyelinase-like phosphodiesterase 3a as molecular markers in colorectal cancer diagnosis

An enzyme-like phosphodiesterase and carbonic anhydrase technology, applied in the field of medicine, can solve the problems of unspecified colorectal cancer screening and diagnosis methods, and achieve the effect of specific standard method system and accurate marker selection.

Pending Publication Date: 2021-12-31
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In summary, there is still insufficient evidence to determine which biomarker has a clear advantage, and

Method used

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  • Application of carbonic anhydrase 1 and acid sphingomyelinase-like phosphodiesterase 3a as molecular markers in colorectal cancer diagnosis
  • Application of carbonic anhydrase 1 and acid sphingomyelinase-like phosphodiesterase 3a as molecular markers in colorectal cancer diagnosis
  • Application of carbonic anhydrase 1 and acid sphingomyelinase-like phosphodiesterase 3a as molecular markers in colorectal cancer diagnosis

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Screening of Candidate Biomarkers by Colorectal Cancer Tissue and Serum Proteomics Experiment

[0031] 1. Sample collection:

[0032] 1) Colorectal cancer case population: patients diagnosed with colorectal cancer by pathology in the Third Affiliated Hospital of Harbin Medical University in 2016, excluding neuroendocrine cancer, malignant melanoma, non-Hodgkin's lymphoma, gastrointestinal stromal tumor , Lynch syndrome colorectal cancer patients, only newly diagnosed pathologically confirmed cases are reserved.

[0033] Collection of tissue samples: 5 colorectal cancer tissues and paired distal normal mucosal tissue samples were collected during the operation, immediately stored in liquid nitrogen, and transferred to a -80°C refrigerator for storage until detection and analysis.

[0034] Serum sample collection: preoperative fasting venous blood sample 5-10ml, a total of 15 cases. After collection, let it stand for 1-2 hours, centrifuge at 3000 rpm for 10 mi...

Embodiment 2

[0055] Example 2 Population verification of diagnostic protein markers for colorectal cancer

[0056] Two proteins CA1 and SMPDL3A specific to colorectal cancer were validated in low-throughput in patients with colorectal cancer and healthy people, their protein expression in serum of cases and controls was detected, and their effects on colorectal cancer were evaluated. diagnostic value.

[0057] 1. Sample collection

[0058] In this study, 108 patients with colorectal cancer pathologically diagnosed in the Third Affiliated Hospital of Harbin Medical University in 2016 and 120 orthopedic patients in the Second Affiliated Hospital of Harbin Medical University in 2017 were included in the control population without cancer. Female participants accounted for 32.4% and 48.4% in the case-control group, of which 53.7% and 36.7% were older than 60 years old, demographic information Table 2.

[0059] Collect 5-10ml of fasting venous blood samples. After collection, let it stand for...

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Abstract

The invention discloses an application of carbonic anhydrase 1 and acid sphingomyelinase-like phosphodiesterase 3a as molecular markers in colorectal cancer diagnosis. The application is characterized by screening the differential biomarkers in the colorectal cancer tissues and the far-end normal tissues and the differential biomarkers in the serum of colorectal cancer patients and the cancer-free control people through a high-throughput chromatography-mass spectrometry-based proteomics technology; screening the colorectal cancer related candidate biomarkers through the comparative analysis of the proteomics results of the tissue and serum samples; verifying the colorectal cancer distinguishing capability of the biomarker through an external database; and finally, detecting the colorectal cancer biomarker of the serum of a case control group by a low-flux method, and evaluating the diagnostic ability of the marker on the colorectal cancer. Based on the screened biomarkers, a rapid, sensitive and specific standard method system for colorectal cancer diagnosis is attempted to be established, and help is provided for the large-scale population screening and clinical diagnosis.

Description

technical field [0001] The invention relates to a biomarker that can be used for the diagnosis of colorectal cancer in a crowd and its application. In particular, it relates to the application of carbonic anhydrase 1 and acid sphingomyelinase-like phosphodiesterase 3a as molecular markers in the diagnosis of colorectal cancer. The invention belongs to the technical field of medicine. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in the world. In 2018, the incidence of colorectal cancer ranked fourth among all cancers, and the mortality rate of colorectal cancer ranked third among all cancers. Among different gender groups, the incidence of colorectal cancer ranks third and second in men and women, and the mortality rate also ranks third and second in men and women, respectively. In the global cancer statistics report from 2019 to 2021, the incidence and mortality of colorectal cancer ranked third among both men and women. [00...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/573
CPCG01N33/6848G01N33/57419G01N33/57488G01N33/573G01N2333/916G01N2333/988
Inventor 王帆王茂清龙智平谢堃
Owner HARBIN MEDICAL UNIVERSITY
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