Light field super-resolution three-dimensional reconstruction method and system

Abstract

The invention discloses a light field super-resolution three-dimensional reconstruction method and system. The method comprises the following steps: (1) taking a static sample as a target, and obtaining high-resolution fact three-dimensional data of the static sample; (2) carrying out analog sampling to obtain low-resolution light field projection matched with light field imaging system hardware and high-resolution light field projection with target resolution; (3) training the super-resolution network model as training data until the model converges to obtain a light field super-resolution reconstruction model; (4) inputting light field image data obtained by a light field imaging system by taking a static or dynamic sample as a target into the light field super-resolution reconstruction model to obtain a light field image with a target resolution; and (5) performing three-dimensional reconstruction to obtain a high-resolution three-dimensional image of a static or dynamic sample. According to the invention, a light field multi-view image with an ultra-diffraction limit can be obtained at an ultra-high speed, and finally microscopic imaging of a dynamic sample, such as a submicron biological dynamic process, is realized.

Description

technical field [0001] The invention belongs to the field of biophoton microscopic imaging, and more specifically relates to a method and system for super-resolution three-dimensional reconstruction of light field. Background technique [0002] Light field microscopy imaging technology has a very high volume imaging rate. Compared with the conventional high-resolution 3D imaging technology that needs to scan and collect multiple 2D images to obtain 3D information, it only needs to collect a single image to obtain the 3D image. Information, which greatly improves the speed of three-dimensional imaging, and provides the possibility for high-speed dynamic imaging of the living body. Light field microscopy technology adds a microlens array to the detection optical path, so that the acquired two-dimensional image can contain both lateral and angular information. However, because this method needs to obtain both lateral and angular information through a single image, the lateral ...

AI Technical Summary

Problems solved by technology

[0004] In view of the above defects or improvement needs of the prior art, the present invention provides a light field super-resolution three-dimensional reconstruction method and system, the purpose of which is to generate a super-diffraction-limited light field image that cannot be obtained by actual shooting through simulation, which is different from actual shooting The obtained light field images are paired, while maintaining the advantage of fast acquisition of 3D information in the light field, the spatial resolution breaks through the diffraction limit of the light field, thus solving the problem that the existing high-resolution 3D imaging technology cannot display dynamic samples. Technical Issues in Micro Imaging

Images