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Method for measuring concentration of furoate in human plasma through HPLC-MS/MS

A blood plasma and concentration technology, applied in the field of analysis, can solve problems such as low detection limit, achieve stable baseline, eliminate matrix effect, and improve recovery rate

Active Publication Date: 2022-01-14
北京阳光德美医药科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

Furthermore, due to the structural characteristics of mometasone furoate, a neutral steroid, it is not easy to be charged in mass spectrometry, and it is easy to form an adduct ion peak under electrospray ionization (ESI) positive ion scanning conditions, and the signal is due to two chlorides. Diluted by the presence of atoms, challenging to achieve low detection limits

Method used

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  • Method for measuring concentration of furoate in human plasma through HPLC-MS/MS
  • Method for measuring concentration of furoate in human plasma through HPLC-MS/MS
  • Method for measuring concentration of furoate in human plasma through HPLC-MS/MS

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Embodiment

[0041] Establishment of an HPLC-MS / MS method for the determination of mometasone furoate concentration in human plasma:

[0042] Preparation of standard and quality control working solutions: Two analysts weighed about 3 mg of mometasone furoate standard substance, added appropriate amount of methanol solution, vortexed, ultrasonically dissolved, and prepared a standard substance stock with a concentration of 0.500 mg / mL liquid, marked MF-Stock. All stock solutions were stored in a refrigerator at 2-8°C for later use; the prepared stock solutions were diluted step by step with 50% acetonitrile, and the specific dilution concentrations were shown in Table 1 below:

[0043] Table 1-1 Mometasone furoate standard curve working solution preparation table

[0044]

[0045]

[0046] Note: The above preparation operations can be adjusted according to actual needs, and the final concentration remains unchanged.

[0047]Table 1-2 Preparation table of mometasone furoate quality c...

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Abstract

The invention discloses a method for detecting the concentration of mometasone furoate in plasma through liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS / MS). The method comprises the following steps: 1, preparing a standard working solution; 2, a standard curve and quality control preparation process; 3, a plasma sample pretreatment process; and 4, HPLC-MS / MS detecting process. The method disclosed by the invention is high in specificity, high in sensitivity and simple to operate, and can meet the analysis requirements of clinical large-batch samples.

Description

technical field [0001] The invention relates to the technical field of analysis, in particular to a method for detecting the concentration of furoate in human plasma Background technique [0002] The chemical name of mometasone furoate is 9,21-dichloro-11b,17-dihydroxy-16a-methylpregna-1,4-diene-3,20-dione 17-(2-furoate ). The chemical structure is C 27 h 30 Cl 2 o 6 , the molecular weight is 521.44. Mometasone furoate is a topical glucocorticoid that does not cause systemic effects at doses that exert local anti-inflammatory effects. After nasal spray administration, the systemic bioavailability of mometasone furoate in plasma was <1%, and after nasal spray inhalation of 200 μg drug, the peak concentration in plasma (C max ) is about 10.0pg / mL, and it needs to be detected with an analytical method with a lower limit of quantitation to calculate the pharmacokinetic parameters. Furthermore, due to the structural characteristics of mometasone furoate, a neutral ster...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/40G01N30/86
CPCG01N30/02G01N30/06G01N30/40G01N30/8679G01N2030/062
Inventor 余丽英马章儒潘晓利郝光涛
Owner 北京阳光德美医药科技有限公司
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