Actinobacillus pleuropneumoniae subunit vaccine

A technology of subunit vaccines and actinobacillus, which is applied in the direction of vaccines, veterinary vaccines, bacterial peptides, etc., to achieve the effect of low overall cost and easy production

Active Publication Date: 2022-01-28
JIANGSU NANNONG HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Vaccination is an important means to prevent and control PCP. Although there are many commercial vaccines on the market, because there are as many as 18 APP serotypes, no vaccine can provide complete protection for APP.

Method used

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  • Actinobacillus pleuropneumoniae subunit vaccine
  • Actinobacillus pleuropneumoniae subunit vaccine
  • Actinobacillus pleuropneumoniae subunit vaccine

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0030] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0031] 1 Material method

[0032] 1.1 Experimental animals

[0033] Sixty-four 4-week-old specific pathogen-free (SPF grade) ICR mice were purchased from Shanghai Xipuer-Bicai Laboratory Animal Co., Ltd.

[0034]1.2 Main reagents

[0035] IgG1 and IgG2a antibodies were purchased from Nanjing Tianwei Company; reverse transcriptase was purchased from Novozyme; restriction endonucleases Sac I, Xho I, Hind III, and protein molecular mass standards were purchased from Thermo Fisher Scientific (China) Company; Mouse Peripheral Blood Lymphocyte Separation Liquid KIT was purchased from Tianjin Haoyang Biological Products Technology Co., Ltd.; Carbomer 971P and ISA-15A wer...

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PUM

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Abstract

The invention discloses an actinobacillus pleuropneumoniae subunit vaccine. The actinobacillus pleuropneumoniae subunit vaccine comprises a protein 1 to 4 combination with amino acid sequences as shown in SEQ ID NO.1 to 4 respectively. The truncated proteins selected in the antigen protein combination are highly homologous in all serotypes of APP, and cross protection can be provided for actinobacillus pleuropneumoniae of different serotypes by preparing the truncated proteins into a subunit vaccine; the truncated proteins are expressed in a soluble form, and are easy to produce and low in cost.

Description

technical field [0001] The invention relates to the field of pleuropneumoniae, in particular to an Actinobacillus pleuropneumoniae subunit vaccine. Background technique [0002] Porcine contagious pleuropneumonia (PCP) is a respiratory infectious disease of pigs. Actinobacillus pleuropneumoniae (APP), a Gram-negative short rod-shaped bacterium, is an important pathogenic factor of PCP. [0003] Vaccination is an important means of preventing and controlling PCP. Although there are many commercial vaccines on the market, because there are as many as 18 APP serotypes, no vaccine can provide complete protection for APP. [0004] In recent years, the invention of APP subunit vaccine has become a hot topic, and it has been confirmed that hemolytic exotoxin (Apx) has good immunogenicity, and the invention provides a subunit vaccine of Actinobacillus pleuropneumoniae , ApxI, ApxII, and ApxIII were truncated and expressed to obtain soluble expressed proteins, and the omp2 protein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/195A61K39/05A61K39/39A61P31/04
CPCC07K14/195A61K39/05A61K39/39A61P31/04A61K2039/552A61K2039/55511Y02A50/30
Inventor 蔡金双李玉峰董彦鹏肖澄周琳车巧林丁文奚胡静雅
Owner JIANGSU NANNONG HI TECH
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