Alternaria brassicae and application thereof

A technology of Alternaria and Brassica, applied in the field of microorganisms, can solve the problems of reduced total yield, poor quality of crops and medicinal plants, and affecting the quality and yield of planted products, and achieves the effect of broad application prospects

Active Publication Date: 2022-01-28
杨凌未来中科环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]Plant diseases have seriously affected the quality and output of planted products. Every year, the total output of domestic and foreign crops and medicinal plants is reduced by more than 10% d

Method used

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  • Alternaria brassicae and application thereof
  • Alternaria brassicae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The source and identification of embodiment 1 Alternaria brassicae (Alternaria brassicae) XL-0010

[0029] ①The source of Alternaria brassicae XL-0010

[0030] On September 4, 2017, a fungus was isolated from Cassia officinalis harvested from the botanical garden of Shenyang Pharmaceutical University in Liaoning Province, named XL-0010.

[0031] ② Identification of strain XL-0010

[0032] See the sequence listing (SEQ ID NO.1) for the ITS sequencing sequence of XL-0010, which has the highest sequence similarity with Sequence ID: MW008951.1, with a similarity of 99.62%.

[0033] According to the above identification results, XL-0010 belongs to Alternaria brassicae.

Embodiment 2

[0034] The main morphological characteristics of embodiment 2 Alternaria brassicae (Alternaria brassicae) XL-0010

[0035] Inoculate Alternaria brassica XL-0010 on potato glucose medium and culture at a constant temperature of 28°C. The initial colony is white, expanding radially, with dense mycelium, round colony, neat edges, and well-developed aerial hyphae. After 3 days, the color of the colonies gradually became darker and gradually turned grayish brown. Conidiophores dark, solitary, of varying lengths, terminal conidia. Conidia dark, with 6-12 transverse septa, 0-6 longitudinal and oblique septa, spore body 64.0-158.0×19.5-38.0 μm, beak 23.0-93.0×6.0-8.0 μm.

[0036] The PDA medium formula: potato dipping powder 3g / L, glucose 20g / L, agar 14g / L, pH value 5.6±0.2.

[0037] See the single colony morphology of the strain on the PDA plate figure 1 , figure 1 -A for figure 1 -A is the front view of the tablet; figure 1 -B is the back view of the tablet.

Embodiment 3

[0038]Embodiment 3 Alternaria brassicae (Alternaria brassicae) XL-0010 measures the bacteriostatic rate of pathogenic fungi

[0039] Adopt plate confrontation method to measure the bacteriostatic ability of fungi to be screened (Alternaria brassicae XL-0010) to test fungi (more than ten strains of pathogenic fungi), and the specific methods are as follows:

[0040] Prepare a PDA plate, culture the fungus to be screened and the fungus for testing respectively, and then use a sterilized 5mm hole puncher to punch holes in the parts where the growth of the two culture media is the same (distance from the edge of the dish). equidistant). On the PDA empty plate, use sterilized tweezers to pick up the perforated medium block, and inoculate two kinds of fungi respectively on the same straight line from the center of the plate about 3cm away, and then measure the antibacterial effect every 24 hours Circle size (cross method). The antibacterial rate was calculated according to the fol...

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Abstract

The invention relates to alternaria brassicae and application thereof, and belongs to the technical field of microorganisms. According to the invention, the alternaria brassicae is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 15, 2021, and the preservation number of the alternaria brassicae is CGMCC No.23045; the liquid culture of the alternaria brassicae strain has a remarkable inhibition effect on three pathogenic fungi including fusarium graminearum, exserohilum turcicum and brown blotch of citrus grandis, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Alternaria brassicae and its application in antibacterial aspects. Background technique [0002] Alternaria brassicae (Alternaria brassicae) belongs to the kingdom of fungi, phylum Ascomycota, subphylum Ascomycota, class Phytomycetes, order Coelosporum, and family Sporobacteriaceae, and is a common species of Alternaria genus. Alternaria spp. is an important group of fungi in the half-known fungus Pleuromycetes. There are many species of fungi in this genus, and their host range and geographical distribution are wide. More than 95% of the species can be facultatively parasitic on plants and are important plant pathogens. Some species are opportunistic pathogens of humans and animals, causing various diseases. Some species can be used as fungicides, herbicides, etc., and are potential biological resources. [0003] Wheat scab is caused by the fungus Fusarium ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 杨小龙王森林刘文宋沙沙吴彦武红帽
Owner 杨凌未来中科环保科技有限公司
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