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MicroRNA related to type 2 diabetes mellitus, and application thereof

A technology of type 2 diabetes and reagents, applied in the field of microRNA and its application

Pending Publication Date: 2022-01-28
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on using microRNA as a target for early diagnosis of type 2 diabetes

Method used

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  • MicroRNA related to type 2 diabetes mellitus, and application thereof
  • MicroRNA related to type 2 diabetes mellitus, and application thereof
  • MicroRNA related to type 2 diabetes mellitus, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Human sample collection:

[0066] (1) Sample group and source: December 2018, in December 2018, in the Xinjiang Uygur Autonomous Region Kashi, Yili Region, the stone, collect 36 cases of normal individual serum samples, 36 cases of obese individual serum samples, diabetes individual serum 12 cases of samples.

[0067] (2) Incorporation and exclusion of samples: Normal group: 40 years old 2 ; Fasting blood glucose <6.1mmol / L; triglyceride (Tg) <1.7 mmol / L; total cholesterol (Tc) <5.17mmol / l; gender: 1: 1 pairing; obese group: 40 years old <50 years old; BMI ≥28kg / m2; fasting blood glucose <6.1mmol / L; gender: 1: 1 pair; T2DM group: 38 years old <50 years old; fasting blood glucose ≥ 7.0mmol / L; gender: 1: 1 pairing.

[0068] (3) Collection and preservation of blood samples: After the sample was collected, 4000 rpm was centrifuged at room temperature for 5 min, and the upper serum was taken into a 1.5 mL RNASE-Free EP tube, and was placed in the -80 ° C refrigerator...

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Abstract

The invention provides microRNA related to type 2 diabetes mellitus, and application thereof, and belongs to the technical field of in-vitro diagnosis and biological medicine. The nucleotide sequence of the microRNA is as shown in SEQ ID NO. 1. The microRNA disclosed by the invention is remarkably and highly expressed in serum samples of obese and diabetes individuals, and the expression of the microRNA is remarkably and positively correlated with fasting blood-glucose of a tested individual. By detecting the expression quantity of circulating microRNA of a patient, the risk of suffering from the type 2 diabetes mellitus of the patient can be predicted in advance, and the type 2 diabetes mellitus can be diagnosed in an early stage. According to the invention, on the basis of culturing a human liver cancer cell line HepG2 cell, a human liver cell L02 and a mouse preadipocyte 3T3-L1 in vitro, after transfecting microRNA-Xmimic or an inhibitor, the influence on the glucose metabolism capability of the cell is observed.

Description

Technical field [0001] The present invention belongs to in the field of in vitro diagnosis and biomedical technology, which relates to a microorna associated with type 2 diabetes and its applications. Background technique [0002] Type 2 diabetes (Diabetes Mellitus Type 2, T2DM), Noninsulin-dependent Diabetes Mellitus, NiddM or Adult Diabetes, is a chronic metabolic disease, mostly in 35-40 After the age, there were more than 90% of patients with diabetes. Early symptoms of type 2 diabetes include hunger, thirst, urinary frequency, skin itching, metabolic abnormalities and weakness of fatigue. However, the type 2 diabetes is hidden, and the early inaction is generally relatively alleviated, concealed, longer, typical diabetic symptoms (three more elements, etc.). Thus, early diagnosis of type 2 diabetes allows patients to get appropriate treatment as soon as possible, thereby reducing the risk of complications. [0003] MicroRNA is a class of non-coding single-stranded RNA molecu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/113C12N15/11A61K31/7088A61K9/00A61P3/10A61P3/04
CPCC12Q1/6883C12N15/113A61K31/7088A61K9/0019A61P3/10A61P3/04C12N2310/141C12Q2600/158C12Q2600/178
Inventor 张君潘重阁王翠喆
Owner SHIHEZI UNIVERSITY
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