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Gene GmFAD2-1B site related to soybean oleic acid content, InDel molecular marker and application

A soybean oleic acid, molecular marker technology, applied in the direction of recombinant DNA technology, DNA/RNA fragment, microbial determination/inspection, etc., can solve the problems of harmful trans fatty acids, easy oxidation, reduced nutritional value, etc., to speed up genetic improvement process, the effect of improving the efficiency of breeding selection

Active Publication Date: 2022-02-01
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the three unsaturated fatty acids (oleic acid, linoleic acid, and linolenic acid), linoleic acid and linolenic acid are polyunsaturated fatty acids with poor stability and easy oxidation during high-temperature processing, which reduces the nutritional value and affects the quality of the oil , the industrial hydrogenation reaction is also prone to produce harmful trans fatty acids

Method used

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  • Gene GmFAD2-1B site related to soybean oleic acid content, InDel molecular marker and application
  • Gene GmFAD2-1B site related to soybean oleic acid content, InDel molecular marker and application
  • Gene GmFAD2-1B site related to soybean oleic acid content, InDel molecular marker and application

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Embodiment 1

[0033] Preparation of experimental materials

[0034] In the long-term research work of the present invention, it was accidentally found in three soybean materials with high oleic acid content (>40%). At the position of chromosome 35,315,798bp, there is an insertion with a fragment size of 10bp. For this allelic variation, use the Primer-BLAST function of NCBI (https: / / www.ncbi.nlm.nih.gov / ) to design InDel marker primers, Upstream primer F1 (Seq ID NO.4) and downstream primer R (Seq ID NO.5). Utilize 51 soybean materials (Table 1) of known oleic acid content to carry out verification, the result figure 1 shown.

[0035] Table 1 Verification of 51 soybean materials with known oleic acid content

[0036]

[0037]

[0038] Note: The above 51 soybean materials are all known public varieties.

[0039] figure 1 Confirmation of electrophoretic patterns of 51 soybean materials for InDel labeling. Such as figure 1 As shown, among the 51 materials, there are 21 soybean mat...

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Abstract

The invention provides a gene GmFAD2-1B site related to the content of soybean oleic acid, an InDel molecular marker and application, and belongs to the field of plant molecular breeding. According to the invention, a gene GmFAD2-1B site related to soybean oleic acid content is located at the positions of 35315798bp of a soybean 20th chromosome, an allele is 10bp insertion / deletion, the site is related to the soybean oleic acid content, and the statistical result of the oleic acid content of a soybean material with the allele being 10bp insertion is remarkably higher than that of a soybean material with the allele being 10bp deletion; and an InDel molecular marker developed on the basis of the site can be used for soybean oleic acid character molecular marker assisted breeding, and has important theoretical and practical guiding significance for accelerating the genetic improvement process of soybean high oleic acid breeding and improving the breeding selection efficiency.

Description

1. Technical field [0001] The invention relates to a GmFAD2-1B site of a gene related to soybean oil content, an InDel molecular marker and its application, and belongs to the field of crop molecular breeding. The InDel molecular marker developed by the invention can select high-oleic acid soybean strains and serve for cultivating new high-oleic acid soybean strains. 2. Background technology [0002] Soybean (Glycine max L.Merrill) originated in China, is one of the world's important oil crops and economic crops, and is also the main source of human vegetable oil and vegetable protein. With the improvement of living standard and improvement of diet structure, people's demand for high-quality soybean oil is increasing day by day, and cultivating high-quality soybean has become one of the important goals of soybean breeding. Soybean oil accounts for about 40% of the vegetable edible oil consumption in my country. The components and proportions of soybean fatty acids determine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 陈华涛张威许文静张红梅崔晓艳刘晓庆王琼陈新
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES