Blood brain barrier injury assessment device and method based on dual-wavelength photoacoustic microscopic imaging

Abstract

The invention discloses a blood-brain barrier injury assessment device and method based on dual-wavelength photoacoustic microscopic imaging, and the device and mehtod are used for realizing high-resolution three-dimensional real-time assessment of blood-brain barrier injury. The device comprises a laser source assembly, a light beam transmission assembly, a light beam scanning assembly, a reflective imaging port assembly, a signal acquisition assembly and a computer. The method comprises the following steps: establishing a blood-brain barrier injury model; injecting a photoacoustic nanoparticle suspension into the blood of the established model through femoral vein; adopting a dual-wavelength photoacoustic microscopic imaging mode to conduct high-resolution brain imaging on the established model to obtain photoacoustic data, wherein pulse laser of a first wavelength and pulse laser of a second wavelength are used for exciting hemoglobin and nanoparticles respectively; reconstructing the photoacoustic data to obtain a capillary network structure of the brain and a distribution condition of nano particles; and identifying the damage area of the blood brain barrier according to the distribution condition of the nano particles on the outer side of the blood vessel, calculating the concentration of the nano particles outside the blood vessel, and analyzing the damage degree quantitatively.

Description

technical field [0001] The invention belongs to the field of medical equipment, and in particular relates to a blood-brain barrier damage assessment device and method based on dual-wavelength photoacoustic microscopic imaging. Background technique [0002] The blood-brain barrier is a multicellular vascular structure composed of endothelial cells and astrocytes, which can strictly regulate the exchange of substances between the blood circulation and the central nervous system, and plays a key role in maintaining the homeostasis of the brain environment . However, in brain diseases such as shock, craniocerebral injury, ischemic stroke, cerebral hemorrhage, and epilepsy, there is often severe damage to the blood-brain barrier, which causes immune proteins and metabolic waste that could not pass through the blood-brain barrier to damage the nervous system. , and then lead to severe neuroinflammation and even neurological function degeneration. Therefore, assessing the blood-b...

AI Technical Summary

Problems solved by technology

However, traditional medical imaging techniques, such as immunohistochemistry, immunofluorescence, confocal microscopy, spectral imaging, fluorescence imaging, CT, MRI, SPECT, etc., are difficult to detect blood-brain barrier damage in the whole brain. High-resolution 3D real-time evaluation

Among them, immunohistochemistry and immunofluorescence require the preparation of isolated brain tissue slices, which cannot be evaluated in vivo; confocal microscopy imaging equipment is expensive, and the point-to-point scanning mechanism limits its imaging speed, making it difficult to achieve whole-brain imaging. real-time assessment of blood-brain barrier damage; spectral imaging lacks depth information and cannot provide a three-dimensional distribution of blood-brain barrier damage; fluorescence imaging has insufficient resolution and is difficult to assess blood-brain barrier damage in a single capillary; CT, MRI and SPECT Imaging equipment is too bulky and expensive, making it difficult to implement and costly to apply, and there is also the problem of insufficient resolution

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