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Polymeric micellar coating for sequential delivery of immunomodulatory factors on nanorod arrayed surface and preparation method and application

An immunoregulatory factor, polymer micelle technology, applied in coating, medical science, prosthesis and other directions, can solve problems such as insufficient osseointegration performance, and achieve the effect of stable process, simple process and simple composition

Active Publication Date: 2022-04-22
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a polymer micelle coating, a preparation method and an application of a nanorod arrayed surface sequentially delivering immunomodulatory factors, to overcome the biological inertia of the surface of titanium and titanium alloy implants in the prior art. Insufficient osseointegration performance, the process of the present invention is stable and highly controllable; the surface layer of the prepared biomedical material can regulate pro-inflammatory (M1 type) and healing-promoting (M2 type) macrophages through the sequential release of immunomodulatory factors Good balance between subpopulations to promote osseointegration performance of the implant after implantation

Method used

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  • Polymeric micellar coating for sequential delivery of immunomodulatory factors on nanorod arrayed surface and preparation method and application
  • Polymeric micellar coating for sequential delivery of immunomodulatory factors on nanorod arrayed surface and preparation method and application
  • Polymeric micellar coating for sequential delivery of immunomodulatory factors on nanorod arrayed surface and preparation method and application

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preparation example Construction

[0038] A method for preparing a nanorod arrayed surface sequentially delivering a polymer micelle coating of immunomodulatory factors, comprising the following steps:

[0039] Step 1: performing micro-arc oxidation on the pure titanium or titanium alloy substrate in an electrolyte containing phosphorus ions and calcium ions, so that a microporous titanium dioxide coating is formed on the surface of the titanium-based substrate;

[0040] Specifically: the pure titanium or titanium alloy substrate is used as the anode, and the stainless steel electrolytic cell is used as the cathode, and micro-arc oxidation is carried out at a temperature of 295-305K. After cleaning and drying with deionized water, a microporous titanium dioxide coating is formed on the surface of the titanium-based substrate. ; The parameters of micro-arc oxidation are: arc frequency 100-120Hz, positive pressure 350-450V, duty cycle 15-25%; electrolyte includes: sodium hydroxide 0.01-0.02mol / L, calcium acetate 0...

Embodiment 1

[0054] A method for preparing biomedical materials coated with polymer micelles for sequentially delivering immunomodulatory factors on the surface of nanorod arrays, comprising the following steps:

[0055] Step 1, micro-arc oxidation of titanium and its alloys:

[0056] The parameters of the micro-arc oxidation are set as follows: the arc frequency of the micro-arc oxidation is 110 Hz, the power supply is a positive voltage of 385 V, and the duty cycle is 20%. In the micro-arc oxidation process, the titanium alloy is used as the anode, and the stainless steel electrolytic cell is used as the cathode. The composition and concentration of the electrolyte are: sodium hydroxide (NaOH) 0.015mol / L, calcium acetate (Ca(CH 3 COO) 2 ) 0.4mol / L, β-glyceride disodium salt pentahydrate (β-GP) 0.04mol / L. During the preparation process, a cooling system is used to control the temperature of the micro-arc oxidation electrolyte at 300K. After micro-arc oxidation, the sample coated with m...

Embodiment 2

[0073] A method for preparing biomedical materials coated with polymer micelles for sequentially delivering immunomodulatory factors on the surface of nanorod arrays, comprising the following steps:

[0074] Step 1, micro-arc oxidation of titanium and its alloys:

[0075] The parameters of the micro-arc oxidation are set as follows: the arc frequency of the micro-arc oxidation is 100 Hz, the power supply is a positive voltage of 350 V, and the duty cycle is 15%. In the micro-arc oxidation process, pure titanium is used as the anode, and the stainless steel electrolytic cell is used as the cathode. The composition and concentration of the electrolyte are: sodium hydroxide (NaOH) 0.02mol / L, calcium acetate (Ca(CH 3 COO) 2 ) 0.3mol / L, β-glyceride disodium salt pentahydrate (β-GP) 0.05mol / L. During the preparation process, a cooling system is used to control the temperature of the micro-arc oxidation electrolyte at 295K. After micro-arc oxidation, the sample coated with micropo...

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Abstract

The invention discloses a polymer micelle coating for sequential delivery of immunoregulatory factors on the surface of nanorod arrays and its preparation method and application. Two kinds of inflammatory factors, S1P with anti-inflammatory effect and LPS with pro-inflammatory effect, will be loaded. The micelles were successively deposited on the array coating of hydroxyapatite nanorods after micro-arc oxidation and hydrothermal treatment by electrodeposition, and the inner and outer double-layer nanomicelles loaded with S1P (inner layer) and LPS (outer layer) were obtained. Immunomodulation composite coating, the composite coating has the following characteristics: it can first release the pro-inflammatory factor LPS deposited on the outer layer, induce macrophages to polarize to pro-inflammatory, and then release the anti-inflammatory factor S1P deposited on the inner layer, induce Macrophages are polarized to promote healing, and the structured surface layer of hydroxyapatite nanorod arrays constructed by bone-like matrix and components can significantly promote the osteogenic behavior of osteoblast-related cells. Osseointegration after implantation.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a polymer micelle coating, a preparation method and an application of nanorod arrayed surface for sequential delivery of immunoregulatory factors. Background technique [0002] Titanium (Ti) and its alloys have high mechanical strength, chemical stability, corrosion resistance and biocompatibility, and are often used to construct orthopedic implants. However, clinically Ti-based implants still have a high failure rate of 15%-20%. In such early failure cases, the reasons for the failure are not only related to the surgical technique and the patient's own health status, but also one of the important reasons for the failure of the implant to achieve osseointegration with the bone tissue of the body at the material-bone interface. The degree of osseointegration of the body's bone tissue will affect the service life of the prosthesis. In fact, Ti is biologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/06A61L27/30A61L27/32A61L27/34A61L27/54A61L27/56
CPCA61L27/06A61L27/306A61L27/32A61L27/54A61L27/56A61L27/34A61L2300/41A61L2300/608A61L2300/232A61L2430/02A61L2300/216A61L2300/412
Inventor 憨勇陈丽冰赵鑫
Owner XI AN JIAOTONG UNIV
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