Preparation method of water-soluble yeast glucan

A yeast glucan, water-soluble technology, applied in the field of glucan

Active Publication Date: 2022-02-08
戚春建
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems in the prior art, the present invention provides a method for preparing water-soluble yeast glucan, which solves the defects of the existing water-soluble yeast glucan, and uses wet planetary ball milling combined with microwave micro-dissolution reaction to prepare yeast glucan Hydroxyl phosphorylation on the sugar surface to achieve water-soluble modification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing water-soluble yeast glucan, comprising the steps of:

[0019] Step 1, adding commercially available yeast glucan with a purity of 99% to absolute ethanol and stirring to form a slurry, and adding calcium hydrogen phosphate to the slurry for sealed planetary wet ball milling to obtain a pretreated slurry; The mass ratio of the yeast dextran to absolute ethanol is 5:1, the stirring speed is 1000r / min, the addition of the calcium hydrogen phosphate is 3 times of the yeast glucan quality, and the pressure of the wet ball milling is 0.8MPa; the planetary wet ball mill uses 3mm steel balls, the power of the planetary ball mill is 800W, and the temperature of the sealed planetary wet ball mill is 80°C. The yeast glucan treated in step 1 contains 55% soluble glucan;

[0020] Step 2, put the pretreatment slurry into water and stir to form a dilution liquid, then slowly introduce carbon dioxide gas and microwave treatment for 2 hours, and obtain the filtra...

Embodiment 2

[0024] A method for preparing water-soluble yeast glucan, comprising the steps of:

[0025] Step 1, adding commercially available yeast glucan with a purity of 99% to absolute ethanol and stirring to form a slurry, and adding calcium hydrogen phosphate to the slurry for sealed planetary wet ball milling to obtain a pretreated slurry; The mass ratio of the yeast dextran to absolute ethanol is 5:3, the stirring speed is 2000r / min, the addition of the calcium hydrogen phosphate is 9 times the quality of the yeast glucan, and the pressure of the wet ball milling is 1.4MPa; The planetary wet ball mill uses 5mm steel balls, the power of the planetary ball mill is 1000W, and the temperature of the sealed planetary wet ball mill is 80°C. The yeast glucan treated in step 1 contains 65% soluble glucan;

[0026]Step 2, put the pretreatment slurry into water and stir to form a dilution liquid, then slowly introduce carbon dioxide gas and microwave treatment for 5h, and obtain the filtrat...

Embodiment 3

[0030] A method for preparing water-soluble yeast glucan, comprising the steps of:

[0031] Step 1, adding commercially available yeast glucan with a purity of 99% to absolute ethanol and stirring to form a slurry, and adding calcium hydrogen phosphate to the slurry for sealed planetary wet ball milling to obtain a pretreated slurry; The mass ratio of the yeast dextran to absolute ethanol is 5:2, the stirring speed is 1500r / min, the addition of the calcium hydrogen phosphate is 8 times the quality of the yeast dextran, and the pressure of the wet ball milling is 1.1MPa; the planetary wet ball mill uses 4mm steel balls, the power of the planetary ball mill is 900W, and the temperature of the sealed planetary wet ball mill is 80°C. The yeast glucan treated in step 1 contains 60% soluble glucan;

[0032] Step 2, putting the pretreatment slurry into water and stirring to form a dilution liquid, then slowly introducing carbon dioxide gas and microwave treatment for 2-5 hours, and ...

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Abstract

The invention belongs to the technical field of glucan, and particularly relates to a preparation method of water-soluble yeast glucan, which comprises the following steps: carrying out water-soluble modification on water-insoluble yeast glucan by using planetary wet ball milling in combination with microwave slightly soluble phosphorylation reaction. According to the preparation method, the defects of the existing water-soluble yeast glucan are overcome, and the hydroxyl on the surface of the yeast glucan is phosphorylated by utilizing wet-process planetary ball milling in combination with a microwave slightly soluble reaction, so that the effect of water-soluble modification is achieved.

Description

technical field [0001] The invention belongs to the technical field of dextran, and in particular relates to a preparation method of water-soluble yeast glucan. Background technique [0002] At present, yeast β-D-glucan (referred to as yeast β-glucan or yeast glucan) is an immunomodulator (non-specific immune enhancer) with good physiological functions, which has the functions of enhancing immunity, anti-tumor, Antibacterial and anti-inflammatory, anti-oxidation, promotion of wound healing, anti-radiation, lowering blood lipids and other physiological effects, but it is insoluble in water and most other solvents, only slightly soluble in dimethyl sulfoxide, which severely limits the application of yeast dextran . [0003] In response to this problem, scholars at home and abroad have carried out modification and modification research on it, mainly based on the modification method. The current modification method is mainly to increase its solubility by connecting these polar ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0024C08B37/0003Y02P20/54
Inventor 戚春建丁骏邵芳宁永玲何流漾王仕忠
Owner 戚春建
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