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Application of circular RNA cGGNBP2

A circular, purpose-built technology, applied in the application of circular RNA cGGNBP2 in the diagnosis, prognosis of intrahepatic cholangiocarcinoma, and the treatment of intrahepatic cholangiocarcinoma, to achieve the effect of inhibiting the proliferation of ICC cells

Active Publication Date: 2022-02-11
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Circular RNA cGGNBP2 (CircBase ID: hsa_circ_0003930) contains the third to sixth exons of the gene GGNBP2, and its circularized nucleotide sequence has 548 bases. Currently, there is no information about circular RNA cGGNBP2 in diagnosis, Reports on the treatment of intrahepatic cholangiocarcinoma and its application in the prognosis of intrahepatic cholangiocarcinoma

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  • Application of circular RNA cGGNBP2
  • Application of circular RNA cGGNBP2
  • Application of circular RNA cGGNBP2

Examples

Experimental program
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Effect test

Embodiment 1

[0046] In this example, the inventor designed a specific primer pair capable of amplifying cGGNBP2 (CircBase ID: hsa_circ_0003930), and used the primer pair to amplify the circular RNA cGGNBP2, and confirmed the splicing position of the circular RNA by the method of generation sequencing After that, cGGNBP2 was determined to be a circular RNA molecule with a closed circular structure through RNase R digestion experiment, and the stability of cGGNBP2 was proved by actinomycin D experiment.

[0047] The experimental scheme of this embodiment is as follows:

[0048] Total RNA was extracted from RBE (human hepatobiliary carcinoma cell) cells using Trizol reagent (Invitrogen, CA, USA), and the purity and concentration of total RNA was detected by ScanDrop100 ultra-micro nucleic acid analyzer. Prepare the reverse transcription reaction system on ice (take the 20 μL system as an example). After the preparation is completed, add the prepared total cellular RNA for reverse transcriptio...

Embodiment 2

[0061] In this example, the difference in expression of cGGNBP2 in tumor tissues and corresponding non-tumor tissues was detected by fluorescent quantitative PCR, and it was found that the expression of circular RNA cGGNBP2 in tumor tissues was significantly higher than that in corresponding non-tumor tissues, indicating that the detection loop can be used to The expression of cGGNBP2-like RNA was used to diagnose intrahepatic cholangiocarcinoma.

[0062] Approved by the Ethical Review Committee of West China Hospital, written informed consent was provided to each patient according to the committee's policy. After the patients were fully informed and signed the informed consent, tumor tissues and paired non-tumor tissues were obtained from the West China hospital from 2009 to 2018. Archives of surgical specimens from 136 ICC patients without any preoperative treatment at the hospital (Sichuan University, Chengdu, China).

[0063] The experimental scheme of this embodiment is a...

Embodiment 3

[0082] In this example, after using the X-tile software to determine the optimal cut-off value of relative cGGNBP2 expression in ICC tissues, 136 ICC patients were divided into a high cGGNBP2 expression group (High cGGNBP2 expression, n=72) and a low cGGNBP2 expression group (Low cGGNBP2 expression, n=64). Table 1 shows the baseline characteristics of the 136 ICC patients.

[0083] Table 1:

[0084]

[0085]

[0086] As shown in Table 1, the increase of cGGNBP2 expression level was significantly correlated with the increase of CA19-9, the increase of tumor volume, the increase of tumor number, lymph node metastasis, and more advanced tumor stage, indicating that circular RNA cGGNBP2 and ICC related to its development.

[0087] Further, as Figure 5 As shown, ICC patients with higher cGGNBP2 expression levels had shorter overall survival (OS) and recurrence-free survival (RFS) after curative resection by Kaplan-Meier survival analysis.

[0088] Table 2 shows the progn...

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Abstract

The invention discloses application of circular RNA cGGNBP2, and provides a kit for diagnosing intrahepatic cholangiocarcinoma and a pharmaceutical composition for treating the intrahepatic cholangiocarcinoma based on the function and mechanism research of the inventor on the circular RNA cGGNBP2 (circBase ID: hsa_circ_0003930) in growth and metastasis of hepatic cholangiocarcinoma cells. The invention also discloses application of the circular RNA cGGNBP2 as a therapeutic target in prognosis, diagnosis and treatment of intrahepatic cholangiocellular carcinoma.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of circular RNA cGGNBP2 in the diagnosis, treatment and prognosis of intrahepatic cholangiocarcinoma. Background technique [0002] Intrahepatic cholangiocarcinoma (ICC) accounts for about 15% to 20% of primary liver cancer, and its incidence has increased significantly in recent years. High metastasis rate and rapid postoperative recurrence contribute to the poor prognosis of intrahepatic cholangiocarcinoma. Enhanced knowledge of key cancer-promoting events will facilitate the development of novel and effective metastasis-targeted therapies and improve the prognosis of patients with intrahepatic cholangiocarcinoma. [0003] Currently, the relationship between inflammation and cancer has received extensive attention. Interleukin (IL)-6, a pleiotropic cytokine produced in the presence of inflammatory stimuli, plays an important role in tumorigenesis and cance...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/6886C12Q1/686C12N15/113C12Q2600/158C12Q2600/118C12N2310/532C12Q2561/113C12Q2563/107C12Q2545/114Y02A50/30
Inventor 吴泓李慧曾勇袁克非兰天
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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