Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of bacteroides vulgarus probiotic CGMCC NO.17140 in preparation of lipid-lowering medicine

A technology of CGMCCNO.17140, common Bacteroides, applied in the field of microorganisms, can solve problems such as side effects

Active Publication Date: 2022-02-18
ICDC CHINA CDC
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main measure for the treatment of hyperlipidemia is to use statins, but long-term use will have side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bacteroides vulgarus probiotic CGMCC NO.17140 in preparation of lipid-lowering medicine
  • Application of bacteroides vulgarus probiotic CGMCC NO.17140 in preparation of lipid-lowering medicine
  • Application of bacteroides vulgarus probiotic CGMCC NO.17140 in preparation of lipid-lowering medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation, screening and identification of Bacteroides vulgaris.

[0033] 1. Isolation of Bacteroides vulgaris

[0034] 1) Prepare the brain-heart infusion agar medium, after autoclaving at 121°C for 15 minutes, cool to 50°C, add 5% defibrillated blood, mix well and pour into a petri dish;

[0035] 2) Take the serially diluted 10 -4 56ul of feces samples were spread on the culture medium of 1);

[0036] 3) Cultivate for 48 hours at 37°C in an anaerobic environment;

[0037] 4) Pick a single colony that is moist and round and raised for transfer.

[0038] 2. Culture preservation

[0039] In this laboratory, the culture medium containing 25% glycerol is used as the bacteria-preserving solution to carry out the cryopreservation of the strains, and the method is as follows:

[0040] 1) Sterilize a 2mL sterile tube at 121°C for 15 minutes by autoclaving for use;

[0041] 2) After the bacteria are continuously transferred on the solid medium for 3 times, add ...

Embodiment 2

[0062] Example 2. In vivo evaluation of the lipid-lowering function of Bacteroides vulgaris CGMCC NO.17140 in rats.

[0063] Twenty-four female SD rats with a body weight of about 200 grams were randomly divided into three groups, one group was given normal maintenance feed, and the other two groups were given high-fat feed (cholesterol 1%, lard 10%, 0.2 cholate, 10% egg yolk pink). Two groups of high-fat feed groups, after one week of feeding, one group was orally given 10 10 CFU CGMCC NO .17140 Bacteroides vulgaris bacteria liquid, one group was given normal saline every other day, the experiment was terminated for five weeks, and serum lipid levels were detected to evaluate the preventive intervention effect of Bacteroides vulgaris CGMCC NO .17140 lipid metabolism disorder model.

[0064] figure 1 Show the effect of Bacteroides vulgaris CGMCC NO.17140 on the weight gain of high-fat model rats. The average weight gain of the rats in the CGMCCNO.17140 intervention group wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of a bacteroides vulgarus probiotic strain in preparation of a lipid-lowering medicine. The strain is named as bacteroides vulgarus during preservation and is preserved in China General Microbiological Culture Collection Center on January 7, 2019, with an accession number being CGMCC No. 17140. The strain is harmless to animals, and has the blood fat regulating effects of remarkably reducing low-density lipoprotein, total cholesterol and triglyceride and increasing high-density lipoprotein.

Description

technical field [0001] The invention relates to a probiotic and its application, belonging to the field of microorganisms. Background technique [0002] Hyperlipidemia is a lipid metabolism disorder disease caused by abnormal fat metabolism or operation, resulting in high total cholesterol, triglycerides and (or) low-density lipoprotein, and (or) low high-density lipoprotein. It is one of the main pathogenic factors of atherosclerosis. In recent years, with the improvement of people's living standard and the change of diet structure, the incidence of hyperlipidemia is increasing year by year. At present, the main measure for treating hyperlipidemia is to use statins, but long-term use will have side effects. [0003] More and more evidences at home and abroad show that the gut microbiota and its metabolites play an important role in regulating cholesterol metabolism disorders. Bacteroides are small Gram-negative, non-spore-forming, obligate anaerobic bacteria. It normall...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/741A61P3/06A61P29/00
CPCA61K35/741A61P3/06A61P29/00Y02A50/30
Inventor 徐建国刘丽云杨晶
Owner ICDC CHINA CDC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com