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Composite biological inoculant for heavy metal contaminated soil remediation as well as preparation method and application thereof

A technology of complex biological bacteria and polluted soil, which is applied in the restoration, application, and fertilizer mixture of polluted soil. Fix or transform problems, achieve good repair effect, enhance the stability of metabolic function, and improve the effect of physical and chemical properties

Pending Publication Date: 2022-02-22
上海康恒环境修复有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the application of microorganisms in the remediation of heavy metal-contaminated soil has significant advantages, there are still the following problems in the relevant patents in order to achieve the desired effect: ①Most microbial remediation reagents are a single genus of bacteria, which cannot be well adapted to the toxic effects of heavy metals and the soil environment. The heavy metals in polymetallic polluted soil cannot be fixed or transformed; ② affected by the soil environment such as soil nutrients, indigenous microbial antagonism, and heavy metal toxicity, the effect of microbial agents on heavy metal polluted soil is poor. Poor persistence

Method used

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  • Composite biological inoculant for heavy metal contaminated soil remediation as well as preparation method and application thereof
  • Composite biological inoculant for heavy metal contaminated soil remediation as well as preparation method and application thereof
  • Composite biological inoculant for heavy metal contaminated soil remediation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Acquisition of contaminated soil

[0026] Heavy metal-contaminated soil was collected from a plot where a chemical plant is located. The total heavy metal content was 1710mg / kg, including Cd: 192mg / kg, Cr(Ⅵ): 175mg / kg, Pb: 1150mg / kg, Sb: 86mg / kg. The original soil was naturally air-dried to remove obvious debris such as stones and residual roots, mixed thoroughly, passed through a 2mm sieve, and stored at room temperature for later use.

[0027] (2) Preparation of composite biological agent

[0028] Preparation of biochar:

[0029] Air-dry the biomass of Enteromorpha, pulverize, grind, pass through a 2mm sieve to obtain Enteromorpha powder; put the Enteromorpha powder into a crucible, compact and cover it, place it in a muffle furnace, and raise it to 400°C at a rate of 4°C / min , kept for 2 hours, cooled naturally, taken out, crushed and passed through a 60-mesh sieve to obtain Enteromorpha biochar.

[0030] Preparation of arbuscular mycorrhizal fungal agent:

...

Embodiment 2

[0041] The difference from Example 1 is: the total content of heavy metals in polluted soil is 2362mg / kg, wherein Cu: 1052mg / kg, Cr(Ⅵ): 116mg / kg, Ni: 372mg / kg, Pb: 705mg / kg; composite biological The preparation method of the inoculum is to get 70 parts of Enteromorpha biochar, 12 parts of Rhizophagus fungi (Rhizophagus) inoculum, 10 parts of Gigaspora fungus (Gigaspora) inoculum, Cladosporium fungus (Cladosporium ) 8 parts of inoculum, mixed evenly; each experimental group added 3% of soil weight biochar, arbuscular mycorrhizal fungus inoculum, and composite biological inoculum to the soil.

[0042]After comparative analysis, it was found that the content of heavy metals in the contaminated soil of each experimental group decreased after adding the restoration agent, and the content of heavy metals in the soil of the compound biological agent group (BC-AMF) decreased the most, Cu, Cr(Ⅵ), The content of Ni and Pb decreased by 36.6%, 62.9%, 42.2% and 47.5% respectively, so the c...

Embodiment 3

[0046] The difference from Examples 1 and 2 is: the total content of heavy metals in polluted soil is 3562mg / kg, of which Cu: 1240mg / kg, Ni: 285mg / kg, Pb: 822mg / kg, Mn: 1126mg / kg; composite biological bacteria The preparation method of agent is to get 60 parts of Enteromorpha biochar, 15 parts of Rhizophagus fungus (Rhizophagus) fungus, 15 parts of Gigaspora fungus (Gigaspora) fungus, Glomus fungus (Cladosporium) fungus (Cladosporium) 10 parts of bacterial agents were uniformly mixed; each experimental group added 5% of soil weight biochar, arbuscular mycorrhizal fungal agents, and compound biological agents to the soil.

[0047] Table 3 embodiment 3 different treatment groups soil heavy metal content

[0048]

[0049] After comparative analysis, it was found that the content of heavy metals in the contaminated soil of each experimental group decreased after adding the repair agent, and the content of heavy metals in the soil of the compound biological agent group (BC-AMF) ...

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Abstract

The invention discloses a composite biological inoculant for heavy metal contaminated soil remediation as well as a preparation method and application thereof. The invention relates to a composite biological inoculant for efficiently remediating heavy metal contaminated soil as well as a preparation method and application of the composite biological agent. The composite biological inoculant is a biochar-arbuscular mycorrhizal fungi composite biological inoculant which comprises the following components in percentage by weight: 60-80% of biochar and 20-40% of arbuscular mycorrhizal fungi. The composite biological inoculant is prepared by the steps of preparing biochar, preparing a fungal inoculant, mixing and the like, and is used for in-situ remediation of heavy metal contaminated soil. The biochar and the microorganisms can effectively adsorb, fix or convert soil heavy metal, meanwhile, the biochar can provide nutrition and a shelter for the microorganisms, and the survival rate of the microorganisms in the complex microbial inoculant is guaranteed, so that through the synergistic effect of the biochar and the microorganisms, the adaptability of the biochar and the microorganisms to the heavy metal is improved, and then the remediation effect on the heavy metal contaminated soil is enhanced.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a composite biological bacterial agent for remediation of heavy metal polluted soil, a preparation method and application thereof. Background technique [0002] The complexity of remediation of heavy metal-contaminated soil means that there is no truly stable and efficient remediation technology that can meet the needs of actual production. The curing method in physical restoration technology uses curing agents and stabilizers to wrap heavy metal ions to form a stable structure, but the curing method is not suitable for long-term application, because heavy metal substances will be released over time and cause secondary pollution to the soil . The leaching method in the chemical remediation technology extracts and separates heavy metals from the soil by converting them into liquid forms through solvents, but this method is not suitable for soils with poor per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40B09C1/00B09C1/10C05G3/80
CPCC09K17/40B09C1/00B09C1/105C05D9/00C05G3/80B09C2101/00C05F11/08
Inventor 刘明郑阳申英杰刘继东陈俊杰王森陈润生胡佳晨王加华王湘徽
Owner 上海康恒环境修复有限公司