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Application of dexrazoxane in preparation of medicine for preventing or treating adverse reaction caused by platinum chemotherapy medicine in chemotherapy

A technology of dexrazoxane and chemotherapy drugs, applied in the field of application of dexrazoxane in the preparation of drugs for the prevention or treatment of adverse reactions caused by platinum-based chemotherapy drugs in chemotherapy, which can solve the unknown and unresolved effect of preventing and treating cardiotoxicity Adverse reactions of platinum-based chemotherapy drugs and other issues to achieve the effect of reducing risks

Active Publication Date: 2022-02-25
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, there is no mature method to solve the adverse reactions caused by platinum-based chemotherapy drugs in clinical practice.
Dexrazoxane has known cardioprotective effects against anthracyclines, but its protection against cisplatin cardiotoxicity remains unknown

Method used

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  • Application of dexrazoxane in preparation of medicine for preventing or treating adverse reaction caused by platinum chemotherapy medicine in chemotherapy
  • Application of dexrazoxane in preparation of medicine for preventing or treating adverse reaction caused by platinum chemotherapy medicine in chemotherapy
  • Application of dexrazoxane in preparation of medicine for preventing or treating adverse reaction caused by platinum chemotherapy medicine in chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Dexrazoxane improves the survival rate of zebrafish embryos on the Tg[myl7:GFP] zebrafish line, the experimental results are shown in figure 1 .

[0031] Selected 24 hpf (hours post-fertilization) zebrafish embryos with normal development were placed in a 24-well plate, 20 eggs were added to each well, and 1 mL of a drug solution prepared to a corresponding concentration was added to the well plate into each well. Place 24-well plates in a constant temperature incubator at 28.5°C for culture. At 96 hpf, use a research-type inverted microscope to count the survival of the embryos, and the embryonic heart stops beating as the criterion for judging death. Finally, according to the survival rate of each drug administered for 72 h, calculate the LC of the embryo at 96 hpf 50 value, the result is as figure 1 shown. This result indicated that the embryo survival rate decreased with the increase of cisplatin concentration, presenting a clear concentration dependence. The me...

Embodiment 2

[0033] Dexrazoxane reduces the adverse reactions of cisplatin on the Tg[myl7:GFP] zebrafish line, see the experimental results figure 2 and image 3 .

[0034]Place the healthy zebrafish embryos in a 24-well plate, add 1 mL of the drug solution prepared to the corresponding concentration into the well plate, and when the embryo is at 72 hpf (48 h after administration), use a mass fraction of 4% The methylcellulose was used to fix the zebrafish sideways on the glass slide, with the body flat and overlapping eyes, placed under a research-type upright microscope, and the embryos were photographed. The EGFP channel was used to shoot the heart of zebrafish, and the number of heartbeats was recorded for 15 s with a video system to obtain the final number of heartbeats per minute. The video video obtained through the video recording system was played frame by frame using Adobe Premiere Pro CC 2018 Adobe Photoshop CS6 software, and the frame at the end diastolic systole of one hear...

Embodiment 3

[0036] Dexrazoxane treats (reduces) cisplatin-induced cytotoxicity on the H9C2 cell line, see the experimental results Figure 4 .

[0037] H9C2 cells were seeded into 96-well plates at a cell density of 6000, 100 μL of medium was added to each well, and the well plates were placed in an incubator for 48 h. The culture medium was discarded, and 100 μL of the drug solution prepared as the experimental concentration was added to each well, and the culture was continued for 24 h. The OD value at 450 nm was measured by the CCK-8 method with a microplate reader, so as to obtain the toxicity curve of the drug (such as Figure 4 A). According to the cytotoxicity curve, determine the drug concentration at which the cell viability value is about 50%, adopt the pretreatment administration method, first give Dex treatment for 24 h, and finally measure the OD value, so as to obtain the viability curve after administration.

[0038] It can be seen that cisplatin decreased the viability o...

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Abstract

The invention provides application of dexrazoxane in preparation of drugs for preventing or treating adverse reactions caused by platinum chemotherapy drugs in chemotherapy, and experimental results show that dexrazoxane can reduce toxicity, including cardiotoxicity, liver and metabolic toxicity, development delay and neurotoxicity, caused by the platinum chemotherapy drugs in chemotherapy. Therefore, when the platinum chemotherapeutic drug is clinically used for chemotherapy, the dexrazoxane and the platinum chemotherapeutic drug can be combined to reduce the risk of related toxicity.

Description

technical field [0001] The invention relates to the application of dexrazoxane in the preparation of drugs for preventing or treating adverse reactions caused by platinum chemotherapy drugs in chemotherapy. Background technique [0002] So far, the technical means of cancer treatment have been developed rapidly, and cancer mortality in most parts of the world has shown a downward trend. However, many anti-tumor drugs can also cause damage to normal cells while treating tumors, and cause a variety of adverse reactions to chemotherapy. Therefore, the prognosis of tumor treatment has become a major concern. There are many kinds of toxicities caused by chemotherapy drugs that have been reported so far. Oncological heart disease is a long-term or short-term complication caused by certain chemotherapy drugs or other biological agents, resulting in clinically irreversible cardiac dysfunction. Oncological heart disease has become a factor affecting the prognosis of cancer and is an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/496A61K33/243A61K31/555A61P9/00A61P1/16A61P25/00A61P39/02A61P3/00
CPCA61K31/496A61K33/243A61K31/555A61P9/00A61P1/16A61P25/00A61P39/02A61P3/00A61K2300/00
Inventor 伦静雯赵海山张艺瀚卢宇靖
Owner GUANGDONG UNIV OF TECH
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