Novel coronavirus rapid PCR detection primer group, detection device and application thereof

Abstract

The invention relates to the technical field of virus detection, and discloses a novel coronavirus rapid PCR detection primer group, a detection device and application thereof, the novel coronavirus rapid PCR detection primers include five primers, namely F3, B3, FIP, BIP and LoopB. the novel coronavirus rapid PCR detection kit comprises a sampling swab, a PCR reagent tube, a lysis solution test tube, an LAMP reaction solution test tube, a primer mixture test tube, a colorless crystal violet solution test tube and a packaging box, the novel coronavirus rapid PCR detection primers are placed in the primer mixture test tube, and six storage grooves are formed in the packaging box. The virus PCR rapid detection device comprises a packaging box, an upper cover plate, a PCR reagent tube base, a PCR reagent tube hole groove, an upper cover heating module and a base heating module, wherein the PCR reagent tube base is mounted in the packaging box. According to the present invention, the rapid cracking of the virus can be achieved, the rapid nucleic acid amplification reaction can be conveniently performed, the detection environment requirement is reduced, and the detection accuracy is improved.

Description

technical field [0001] The present invention relates to the technical field of virus detection, in particular to a novel coronavirus rapid PCR detection primer set, detection device and application thereof. Background technique [0002] The rapid and efficient detection of viruses is of great significance for early detection and prevention of virus infection. PCR nucleic acid molecular diagnosis technology is an important method in virus detection. The detection process can be divided into nucleic acid extraction, PCR amplification reaction, qualitative amplification product, result judgment and issuance of completion report. For respiratory tract infections, the samples that patients are more likely to collect themselves are throat swabs, nasal swabs, saliva and sputum, etc. However, it is necessary to extract and purify the nucleic acid from the collected samples in the central laboratory before performing RT-PCR detection. However, this process is relatively tedious and...

AI Technical Summary

Problems solved by technology

However, this process is relatively cumbersome and time-consuming, and it needs to be operated in a corresponding level of laboratory

[0003] In addition, certain temperature conditions and detection time need to be met in PCR nucleic acid molecular diagnostic testing. In recent years, although some PCR automated nucleic acid molecular diagnostic systems have appeared on the market, such as GeneXpert and FilmArray, the automatic movement components of these systems and used for PCR The amplified precise temperature control modules are relatively complicated, and generally have the problems of bulky, complicated operation and high price

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